TOX3_RAT
ID TOX3_RAT Reviewed; 577 AA.
AC B7SBD2;
DT 31-MAY-2011, integrated into UniProtKB/Swiss-Prot.
DT 10-FEB-2009, sequence version 1.
DT 25-MAY-2022, entry version 62.
DE RecName: Full=TOX high mobility group box family member 3;
DE AltName: Full=Trinucleotide repeat-containing gene 9 protein;
GN Name=Tox3; Synonyms=Tnrc9;
OS Rattus norvegicus (Rat).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Rattus.
OX NCBI_TaxID=10116;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, INTERACTION WITH CREB1 AND CREBBP,
RP TISSUE SPECIFICITY, AND DEVELOPMENTAL STAGE.
RC TISSUE=Brain;
RX PubMed=19196971; DOI=10.1073/pnas.0805555106;
RA Yuan S.H., Qiu Z., Ghosh A.;
RT "TOX3 regulates calcium-dependent transcription in neurons.";
RL Proc. Natl. Acad. Sci. U.S.A. 106:2909-2914(2009).
CC -!- FUNCTION: Transcriptional coactivator of the p300/CBP-mediated
CC transcription complex. Activates transactivation through cAMP response
CC element (CRE) sites. Protects against cell death by inducing
CC antiapoptotic and repressing pro-apoptotic transcripts. Stimulates
CC transcription from the estrogen-responsive or BCL-2 promoters. Required
CC for depolarization-induced transcription activation of the C-FOS
CC promoter in neurons. Associates with chromatin to the estrogen-
CC responsive C3 promoter region. {ECO:0000269|PubMed:19196971}.
CC -!- SUBUNIT: Homodimer. Interacts (via HGM box) with CITED1 (via C-
CC terminus); the interaction increases estrogen-response element (ERE)-
CC dependent transcription and protection against cell death. Interacts
CC with CREB1 (phosphorylated form) (By similarity). Interacts with CREB1;
CC the interaction is not depolarization dependent. Interacts with CREBBP
CC (via C-terminus). {ECO:0000250, ECO:0000269|PubMed:19196971}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000305}.
CC -!- TISSUE SPECIFICITY: Expressed in the cortex and cerebellum.
CC {ECO:0000269|PubMed:19196971}.
CC -!- DEVELOPMENTAL STAGE: Expressed in the cortex and cerebellum at 18 dpc.
CC {ECO:0000269|PubMed:19196971}.
CC -!- DOMAIN: the C-terminus is required for calcium responsiveness but not
CC for transactivation activity.
CC -!- DOMAIN: The N-terminus is absolutely necessary for transactivation
CC activity.
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DR EMBL; EU194254; ABX76291.1; -; mRNA.
DR AlphaFoldDB; B7SBD2; -.
DR SMR; B7SBD2; -.
DR STRING; 10116.ENSRNOP00000034957; -.
DR PaxDb; B7SBD2; -.
DR PeptideAtlas; B7SBD2; -.
DR RGD; 1311529; Tox3.
DR eggNOG; KOG0381; Eukaryota.
DR InParanoid; B7SBD2; -.
DR PhylomeDB; B7SBD2; -.
DR PRO; PR:B7SBD2; -.
DR Proteomes; UP000002494; Unplaced.
DR GO; GO:0005634; C:nucleus; IBA:GO_Central.
DR GO; GO:0003682; F:chromatin binding; ISS:UniProtKB.
DR GO; GO:0031490; F:chromatin DNA binding; IBA:GO_Central.
DR GO; GO:0051219; F:phosphoprotein binding; ISO:RGD.
DR GO; GO:0042803; F:protein homodimerization activity; ISS:UniProtKB.
DR GO; GO:0003713; F:transcription coactivator activity; ISO:RGD.
DR GO; GO:0006915; P:apoptotic process; IEA:UniProtKB-KW.
DR GO; GO:0019722; P:calcium-mediated signaling; IDA:UniProtKB.
DR GO; GO:0043524; P:negative regulation of neuron apoptotic process; ISS:UniProtKB.
DR GO; GO:0045944; P:positive regulation of transcription by RNA polymerase II; ISO:RGD.
DR GO; GO:0045893; P:positive regulation of transcription, DNA-templated; IDA:UniProtKB.
DR GO; GO:0042981; P:regulation of apoptotic process; ISS:UniProtKB.
DR GO; GO:0006357; P:regulation of transcription by RNA polymerase II; IBA:GO_Central.
DR Gene3D; 1.10.30.10; -; 1.
DR InterPro; IPR009071; HMG_box_dom.
DR InterPro; IPR036910; HMG_box_dom_sf.
DR Pfam; PF00505; HMG_box; 1.
DR SMART; SM00398; HMG; 1.
DR SUPFAM; SSF47095; SSF47095; 1.
DR PROSITE; PS50118; HMG_BOX_2; 1.
PE 1: Evidence at protein level;
KW Activator; Apoptosis; DNA-binding; Nucleus; Reference proteome;
KW Transcription; Transcription regulation.
FT CHAIN 1..577
FT /note="TOX high mobility group box family member 3"
FT /id="PRO_0000409384"
FT DNA_BIND 254..322
FT /note="HMG box"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00267"
FT REGION 188..258
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 470..490
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 518..577
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 188..217
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 218..239
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 577 AA; 63503 MW; 1D9FC288446695DE CRC64;
MDVRFYPAAA GDPAGLDFAQ CLGYYGYSKL GNNNYMNMAE ANNAFFAASE QTFHTPSLGD
EEFEIPPITP PPESDPTLGM PDVLLPFQTL SDPLPSQGNE FTPQFPPQSL DLPSITISRN
LVEQDGVLHS NGLHMDQSHT QVSQYRQDPS LVMRSIVHMT DAARSGIMPP AQLTTINQSQ
LSAQLGLNLG GASVPHTSPS PPASKSATPS PSSSINEEDA DETNRAVGEK RTAPDSGKKP
KTPKKKKKKD PNEPQKPVSA YALFFRDTQA AIKGQNPNAT FGEVSKIVAS MWDSLGEEQK
QVYKRKTEAA KKEYLKALAA YRASLVSKAA AESAEAQTIR SVQQTLASTN LTSSLLLNTS
LSQHGTVPAS PQTLPQSLPR SIAPKPLTMR LPMSQIVTSV TIAANMPSNI GAPLISSMGT
TMVGSVSSTQ VSPSVQTQQH QLQLQQQQQQ QQQQMQQMQH QQLQQHQMHQ QIQQQMQQQH
FQHHMQQHLQ QQQQQHLQQQ ISQQQLQQQL QQHLQLQQQL QHMQHQSQPS PRQHSPVTSQ
ITSPIPAIGS PQPASQQHQP QIQSQTQTQV LPQVSIF
