TOX5_BORPE
ID TOX5_BORPE Reviewed; 133 AA.
AC P04981;
DT 13-AUG-1987, integrated into UniProtKB/Swiss-Prot.
DT 13-AUG-1987, sequence version 1.
DT 25-MAY-2022, entry version 127.
DE RecName: Full=Pertussis toxin subunit 5;
DE Short=PTX S5;
DE AltName: Full=Islet-activating protein S5;
DE Short=IAP S5;
DE Flags: Precursor;
GN Name=ptxE; OrderedLocusNames=BP3786;
OS Bordetella pertussis (strain Tohama I / ATCC BAA-589 / NCTC 13251).
OC Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
OC Alcaligenaceae; Bordetella.
OX NCBI_TaxID=257313;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=BP165;
RX PubMed=2873570; DOI=10.1073/pnas.83.13.4631;
RA Nicosia A., Perugini M., Franzini C., Casagli M.C., Borri M.G., Antoni G.,
RA Almoni M., Neri P., Ratti G., Rappuoli R.;
RT "Cloning and sequencing of the pertussis toxin genes: operon structure and
RT gene duplication.";
RL Proc. Natl. Acad. Sci. U.S.A. 83:4631-4635(1986).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX PubMed=3704651; DOI=10.1126/science.3704651;
RA Locht C., Keith J.M.;
RT "Pertussis toxin gene: nucleotide sequence and genetic organization.";
RL Science 232:1258-1264(1986).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Tohama I / ATCC BAA-589 / NCTC 13251;
RX PubMed=12910271; DOI=10.1038/ng1227;
RA Parkhill J., Sebaihia M., Preston A., Murphy L.D., Thomson N.R.,
RA Harris D.E., Holden M.T.G., Churcher C.M., Bentley S.D., Mungall K.L.,
RA Cerdeno-Tarraga A.-M., Temple L., James K.D., Harris B., Quail M.A.,
RA Achtman M., Atkin R., Baker S., Basham D., Bason N., Cherevach I.,
RA Chillingworth T., Collins M., Cronin A., Davis P., Doggett J., Feltwell T.,
RA Goble A., Hamlin N., Hauser H., Holroyd S., Jagels K., Leather S.,
RA Moule S., Norberczak H., O'Neil S., Ormond D., Price C., Rabbinowitsch E.,
RA Rutter S., Sanders M., Saunders D., Seeger K., Sharp S., Simmonds M.,
RA Skelton J., Squares R., Squares S., Stevens K., Unwin L., Whitehead S.,
RA Barrell B.G., Maskell D.J.;
RT "Comparative analysis of the genome sequences of Bordetella pertussis,
RT Bordetella parapertussis and Bordetella bronchiseptica.";
RL Nat. Genet. 35:32-40(2003).
RN [4]
RP X-RAY CRYSTALLOGRAPHY (2.9 ANGSTROMS).
RC STRAIN=10536;
RX PubMed=8075982; DOI=10.1016/s0969-2126(00)00007-1;
RA Stein P.E., Boodhoo A., Armstrong G.D., Cockle S.A., Klein M.H., Read R.J.;
RT "The crystal structure of pertussis toxin.";
RL Structure 2:45-57(1994).
RN [5]
RP X-RAY CRYSTALLOGRAPHY (2.7 ANGSTROMS).
RX PubMed=8637000; DOI=10.1006/jmbi.1996.0277;
RA Hazes B., Boodhoo A., Cockle S.A., Read R.J.;
RT "Crystal structure of the pertussis toxin-ATP complex: a molecular
RT sensor.";
RL J. Mol. Biol. 258:661-671(1996).
CC -!- FUNCTION: PTX oligomer B binds to receptors on the eukaryotic cell
CC surface and facilitates the translocation of the toxic subunit across
CC the cell membrane.
CC -!- SUBUNIT: Pertussis toxin contains five different chains, S1-S5. They
CC are organized into 2 functional subunits: A, composed of S1 (which is
CC toxic) and B, containing S2, S3, S5, and two copies of S4 (B binds to
CC the membrane receptors). Dimers of S2-S4 and S3-S4 are held together by
CC S5.
CC -!- SUBCELLULAR LOCATION: Secreted. Host cell membrane {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAA22984.1; Type=Erroneous initiation; Evidence={ECO:0000305};
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DR EMBL; M14378; AAA83983.1; -; Genomic_DNA.
DR EMBL; M13223; AAA22984.1; ALT_INIT; Genomic_DNA.
DR EMBL; BX640422; CAE44041.1; -; Genomic_DNA.
DR PIR; E24394; WEBR51.
DR RefSeq; NP_882285.1; NC_002929.2.
DR PDB; 1BCP; X-ray; 2.70 A; F/L=35-133.
DR PDB; 1PRT; X-ray; 2.90 A; F/L=36-133.
DR PDB; 1PTO; X-ray; 3.50 A; F/L=36-133.
DR PDB; 6RO0; X-ray; 2.13 A; F/L=1-133.
DR PDBsum; 1BCP; -.
DR PDBsum; 1PRT; -.
DR PDBsum; 1PTO; -.
DR PDBsum; 6RO0; -.
DR AlphaFoldDB; P04981; -.
DR SMR; P04981; -.
DR STRING; 257313.BP3786; -.
DR TCDB; 1.C.72.1.1; the pertussis toxin (ptx) family.
DR KEGG; bpe:BP3786; -.
DR PATRIC; fig|257313.5.peg.4090; -.
DR HOGENOM; CLU_2045172_0_0_4; -.
DR OMA; TAFMPGR; -.
DR EvolutionaryTrace; P04981; -.
DR Proteomes; UP000002676; Chromosome.
DR GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR GO; GO:0020002; C:host cell plasma membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0016020; C:membrane; IEA:UniProtKB-KW.
DR GO; GO:0090729; F:toxin activity; IEA:UniProtKB-KW.
DR InterPro; IPR008992; Enterotoxin.
DR InterPro; IPR015356; Pertussis_toxin_subS5.
DR Pfam; PF09276; Pertus-S5-tox; 1.
DR SUPFAM; SSF50203; SSF50203; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Disulfide bond; Host cell membrane; Host membrane; Membrane;
KW Reference proteome; Secreted; Signal; Toxin; Virulence; Whooping cough.
FT SIGNAL 1..34
FT CHAIN 35..133
FT /note="Pertussis toxin subunit 5"
FT /id="PRO_0000019364"
FT DISULFID 61..75
FT DISULFID 126..132
FT STRAND 38..54
FT /evidence="ECO:0007829|PDB:6RO0"
FT STRAND 57..65
FT /evidence="ECO:0007829|PDB:6RO0"
FT STRAND 67..69
FT /evidence="ECO:0007829|PDB:1PTO"
FT STRAND 72..77
FT /evidence="ECO:0007829|PDB:6RO0"
FT HELIX 86..99
FT /evidence="ECO:0007829|PDB:6RO0"
FT STRAND 103..109
FT /evidence="ECO:0007829|PDB:6RO0"
FT STRAND 112..116
FT /evidence="ECO:0007829|PDB:1PRT"
FT STRAND 118..125
FT /evidence="ECO:0007829|PDB:6RO0"
SQ SEQUENCE 133 AA; 14500 MW; 4DDC39CEC7D9D28D CRC64;
MQRQAGLPLK ANPMHTIASI LLSVLGIYSP ADVAGLPTHL YKNFTVQELA LKLKGKNQEF
CLTAFMSGRS LVRACLSDAG HEHDTWFDTM LGFAISAYAL KSRIALTVED SPYPGTPGDL
LELQICPLNG YCE
