ID   TOXK_CYBSA              Reviewed;         124 AA.
AC   Q00948;
DT   27-MAY-2002, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1996, sequence version 1.
DT   25-MAY-2022, entry version 68.
DE   RecName: Full=Killer toxin;
DE   Flags: Precursor;
GN   Name=HSK;
OS   Cyberlindnera saturnus (Yeast) (Williopsis saturnus).
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC   Saccharomycetales; Phaffomycetaceae; Cyberlindnera.
OX   NCBI_TaxID=907340;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=NBRC 0117 / IAM 12217 / JCM 3623 / NCYC 23;
RX   PubMed=8299957; DOI=10.1016/0378-1119(93)90018-x;
RA   Kimura T., Kitamoto N., Matsuoka K., Nakamura K., Iimura Y., Kito Y.;
RT   "Isolation and nucleotide sequences of the genes encoding killer toxins
RT   from Hansenula mrakii and H. saturnus.";
RL   Gene 137:265-270(1993).
CC   -!- FUNCTION: This toxin kills sensitive strains of yeast.
CC   -!- SUBUNIT: Monomer. {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Secreted {ECO:0000250}.
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DR   EMBL; D13446; BAA02705.1; -; Genomic_DNA.
DR   AlphaFoldDB; Q00948; -.
DR   SMR; Q00948; -.
DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR   GO; GO:0090729; F:toxin activity; IEA:UniProtKB-KW.
DR   GO; GO:0008219; P:cell death; IEA:InterPro.
DR   Gene3D; 2.60.20.20; -; 1.
DR   InterPro; IPR011024; G_crystallin-like.
DR   InterPro; IPR015290; Yeast-kill-tox.
DR   InterPro; IPR038651; Yeast_kill_tox_sf.
DR   Pfam; PF09207; Yeast-kill-tox; 1.
DR   SUPFAM; SSF49695; SSF49695; 1.
PE   3: Inferred from homology;
KW   Cleavage on pair of basic residues; Disulfide bond; Secreted; Signal;
KW   Toxin.
FT   SIGNAL          1..19
FT                   /evidence="ECO:0000255"
FT   PROPEP          20..37
FT                   /evidence="ECO:0000250"
FT                   /id="PRO_0000022570"
FT   CHAIN           38..124
FT                   /note="Killer toxin"
FT                   /id="PRO_0000022571"
FT   DISULFID        45..55
FT                   /evidence="ECO:0000250"
FT   DISULFID        48..108
FT                   /evidence="ECO:0000250"
FT   DISULFID        64..94
FT                   /evidence="ECO:0000250"
FT   DISULFID        85..102
FT                   /evidence="ECO:0000250"
FT   DISULFID        103..109
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   124 AA;  13347 MW;  DD7C394D64FE7E86 CRC64;
     MKVSLVYGLT CFLDATSSAL PSAMAAANSE SSALEKRNDG YLVMCKNCDP NSGSCDWKQN
     WNTCVGIGSN VHWMVTGESN GQQGCAIIWE GSGCTGRSTT MCCPGDTCCN INTGFYIRSY
     RRVE