ID   TOXK_MILFA              Reviewed;         222 AA.
AC   P19972;
DT   01-FEB-1991, integrated into UniProtKB/Swiss-Prot.
DT   29-AUG-2001, sequence version 4.
DT   25-MAY-2022, entry version 91.
DE   RecName: Full=Salt-mediated killer protoxin 1;
DE   Contains:
DE     RecName: Full=Salt-mediated killer toxin 1 alpha chain;
DE              Short=SMK toxin alpha chain;
DE   Contains:
DE     RecName: Full=Salt-mediated killer toxin 1 gamma chain;
DE              Short=SMK toxin gamma chain;
DE   Contains:
DE     RecName: Full=Salt-mediated killer toxin 1 beta chain;
DE              Short=SMK toxin beta chain;
DE   Flags: Precursor;
GN   Name=SMK1;
OS   Millerozyma farinosa (Yeast) (Pichia farinosa).
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC   Saccharomycetales; Debaryomycetaceae; Millerozyma.
OX   NCBI_TaxID=4920;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND PARTIAL PROTEIN SEQUENCE.
RC   STRAIN=KK1;
RX   PubMed=8300637; DOI=10.1016/s0021-9258(17)42044-8;
RA   Suzuki C., Nikkuni S.;
RT   "The primary and subunit structure of a novel type killer toxin produced by
RT   a halotolerant yeast, Pichia farinosa.";
RL   J. Biol. Chem. 269:3041-3046(1994).
RN   [2]
RP   SEQUENCE REVISION TO 87; 137; 162; 167 AND 206.
RA   Suzuki C., Nikkuni S.;
RL   Submitted (FEB-2000) to the EMBL/GenBank/DDBJ databases.
RN   [3]
RP   PRELIMINARY PROTEIN SEQUENCE OF 146-167.
RC   STRAIN=KK1;
RA   Suzuki C., Nikkuni S.;
RT   "Purification and properties of the killer toxin produced by a halotolerant
RT   yeast, Pichia farinosa.";
RL   Agric. Biol. Chem. 53:2599-2604(1989).
RN   [4]
RP   X-RAY CRYSTALLOGRAPHY (1.8 ANGSTROMS).
RC   STRAIN=KK1;
RX   PubMed=9016714; DOI=10.1016/s0969-2126(97)00168-8;
RA   Kashiwagi T., Kunishima N., Suzuki C., Tsuchiya F., Nikkuni S., Arata Y.,
RA   Morikawa K.;
RT   "The novel acidophilic structure of the killer toxin from halotolerant
RT   yeast demonstrates remarkable folding similarity with a fungal killer
RT   toxin.";
RL   Structure 5:81-94(1997).
CC   -!- FUNCTION: This toxin kills sensitive strains of yeast.
CC   -!- SUBUNIT: Heterodimer of an alpha and a beta chain. The fate of the
CC       gamma chain is unknown.
CC   -!- MISCELLANEOUS: The full activity of this toxin depends on NaCl or KCL.
CC       Its maximum activity is found at 2M salt solution.
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DR   EMBL; D14538; BAA03405.2; -; Genomic_DNA.
DR   PIR; A49995; A49995.
DR   PIR; PQ0007; PQ0007.
DR   PDB; 1KVD; X-ray; 1.80 A; A/C=19-81, B/D=146-222.
DR   PDB; 1KVE; X-ray; 1.80 A; A/C=19-81, B/D=146-222.
DR   PDBsum; 1KVD; -.
DR   PDBsum; 1KVE; -.
DR   AlphaFoldDB; P19972; -.
DR   SMR; P19972; -.
DR   EvolutionaryTrace; P19972; -.
DR   GO; GO:0005576; C:extracellular region; IDA:CAFA.
DR   GO; GO:0032991; C:protein-containing complex; IDA:CAFA.
DR   GO; GO:0090729; F:toxin activity; IDA:CAFA.
DR   GO; GO:0031640; P:killing of cells of another organism; IDA:CAFA.
DR   CDD; cd12839; Killer_toxin_beta; 1.
DR   DisProt; DP00180; -.
DR   Gene3D; 3.30.44.10; -; 1.
DR   Gene3D; 4.10.420.10; -; 1.
DR   InterPro; IPR011329; Killer_tox_Kp4/SMK.
DR   InterPro; IPR011327; Killer_tox_SMK_b.
DR   InterPro; IPR038431; Killer_tox_SMK_b_sf.
DR   InterPro; IPR011328; SMK_a.
DR   SUPFAM; SSF55221; SSF55221; 2.
PE   1: Evidence at protein level;
KW   3D-structure; Cleavage on pair of basic residues;
KW   Direct protein sequencing; Disulfide bond; Glycoprotein; Signal; Toxin.
FT   SIGNAL          1..18
FT   CHAIN           19..81
FT                   /note="Salt-mediated killer toxin 1 alpha chain"
FT                   /id="PRO_0000022565"
FT   CHAIN           82..145
FT                   /note="Salt-mediated killer toxin 1 gamma chain"
FT                   /id="PRO_0000022566"
FT   CHAIN           146..222
FT                   /note="Salt-mediated killer toxin 1 beta chain"
FT                   /id="PRO_0000022567"
FT   CARBOHYD        127
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
FT   DISULFID        37..53
FT   DISULFID        190..219
FT   STRAND          20..28
FT                   /evidence="ECO:0007829|PDB:1KVD"
FT   HELIX           43..45
FT                   /evidence="ECO:0007829|PDB:1KVE"
FT   HELIX           49..71
FT                   /evidence="ECO:0007829|PDB:1KVD"
FT   STRAND          75..80
FT                   /evidence="ECO:0007829|PDB:1KVD"
FT   STRAND          150..159
FT                   /evidence="ECO:0007829|PDB:1KVD"
FT   HELIX           167..180
FT                   /evidence="ECO:0007829|PDB:1KVD"
FT   TURN            181..184
FT                   /evidence="ECO:0007829|PDB:1KVD"
FT   STRAND          187..195
FT                   /evidence="ECO:0007829|PDB:1KVD"
FT   STRAND          198..207
FT                   /evidence="ECO:0007829|PDB:1KVD"
SQ   SEQUENCE   222 AA;  23000 MW;  B1954761060FE24B CRC64;
     MRKETLIGLA FITANVIAWS LRWRMQKSTT IAAIAGCSGA ATFGGLAGGI VGCIAAGILA
     ILQGFEVNWH NGGGGDRSNP VKRSSDSFSI VNHNGEKVDS YAHLVPGKVG KVIIDNIELS
     AIRYANNHTS LGYHFTSDGS GPAARGEATT IWGVGADEAI DKGTPSKNDL QNMSADLAKN
     GFKGHQGVAC STVKDGNKDV YMIKFSLAGG SNDPGGSPCS DD