TOXR_VIBCH
ID TOXR_VIBCH Reviewed; 294 AA.
AC P15795; Q9KTB9;
DT 01-APR-1990, integrated into UniProtKB/Swiss-Prot.
DT 01-DEC-2000, sequence version 2.
DT 03-AUG-2022, entry version 133.
DE RecName: Full=Cholera toxin transcriptional activator;
GN Name=toxR; OrderedLocusNames=VC_0984;
OS Vibrio cholerae serotype O1 (strain ATCC 39315 / El Tor Inaba N16961).
OC Bacteria; Proteobacteria; Gammaproteobacteria; Vibrionales; Vibrionaceae;
OC Vibrio.
OX NCBI_TaxID=243277;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=ATCC 25870 / Classical Inaba 569B / Serotype O1;
RX PubMed=3802195; DOI=10.1016/0092-8674(87)90430-2;
RA Miller V.L., Taylor R.K., Mekalanos J.J.;
RT "Cholera toxin transcriptional activator toxR is a transmembrane DNA
RT binding protein.";
RL Cell 48:271-279(1987).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 39315 / El Tor Inaba N16961;
RX PubMed=10952301; DOI=10.1038/35020000;
RA Heidelberg J.F., Eisen J.A., Nelson W.C., Clayton R.A., Gwinn M.L.,
RA Dodson R.J., Haft D.H., Hickey E.K., Peterson J.D., Umayam L.A., Gill S.R.,
RA Nelson K.E., Read T.D., Tettelin H., Richardson D.L., Ermolaeva M.D.,
RA Vamathevan J.J., Bass S., Qin H., Dragoi I., Sellers P., McDonald L.A.,
RA Utterback T.R., Fleischmann R.D., Nierman W.C., White O., Salzberg S.L.,
RA Smith H.O., Colwell R.R., Mekalanos J.J., Venter J.C., Fraser C.M.;
RT "DNA sequence of both chromosomes of the cholera pathogen Vibrio
RT cholerae.";
RL Nature 406:477-483(2000).
RN [3]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-11.
RC STRAIN=ATCC 55056 / El Tor Ogawa E7946;
RX PubMed=2124707; DOI=10.1073/pnas.87.24.9898;
RA Parsot C., Mekalanos J.J.;
RT "Expression of ToxR, the transcriptional activator of the virulence factors
RT in Vibrio cholerae, is modulated by the heat shock response.";
RL Proc. Natl. Acad. Sci. U.S.A. 87:9898-9902(1990).
RN [4]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 263-294.
RX PubMed=1898871; DOI=10.1016/0092-8674(91)90206-e;
RA DiRita V.J., Mekalanos J.J.;
RT "Periplasmic interaction between two membrane regulatory proteins, ToxR and
RT ToxS, results in signal transduction and transcriptional activation.";
RL Cell 64:29-37(1991).
RN [5]
RP MUTAGENESIS.
RX PubMed=1400230; DOI=10.1128/jb.174.21.6807-6814.1992;
RA Ottemann K.M., Dirita V.J., Mekalanos J.J.;
RT "ToxR proteins with substitutions in residues conserved with OmpR fail to
RT activate transcription from the cholera toxin promoter.";
RL J. Bacteriol. 174:6807-6814(1992).
CC -!- FUNCTION: This transcription activator controls cholera toxin, pilus
CC colonization factor and outer membrane protein expression in
CC V.cholerae. It binds to the 5'-TTTTGAT-3' tandemly repeated DNA
CC sequence in the cholera toxin promoter region. ToxS interacts with the
CC C-terminal periplasmic domain of ToxR, stimulating its activity. It
CC activates transcription at the promoters for tcpI and tcpA and this is
CC presumably via ToxT.
CC -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000305}; Single-pass membrane
CC protein {ECO:0000305}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; M21249; AAA27549.1; -; Genomic_DNA.
DR EMBL; AE003852; AAF94145.1; -; Genomic_DNA.
DR EMBL; M58033; AAA27575.1; -; Genomic_DNA.
DR EMBL; M62761; AAA63558.1; -; Genomic_DNA.
DR PIR; A25970; A25970.
DR PIR; E82257; E82257.
DR RefSeq; NP_230630.1; NC_002505.1.
DR AlphaFoldDB; P15795; -.
DR SMR; P15795; -.
DR STRING; 243277.VC_0984; -.
DR PRIDE; P15795; -.
DR DNASU; 2614237; -.
DR EnsemblBacteria; AAF94145; AAF94145; VC_0984.
DR KEGG; vch:VC_0984; -.
DR PATRIC; fig|243277.26.peg.937; -.
DR eggNOG; COG3710; Bacteria.
DR HOGENOM; CLU_085388_0_0_6; -.
DR OMA; HEFVWRE; -.
DR BioCyc; VCHO:VC0984-MON; -.
DR PHI-base; PHI:6217; -.
DR PHI-base; PHI:701; -.
DR PHI-base; PHI:7777; -.
DR Proteomes; UP000000584; Chromosome 1.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR GO; GO:0000156; F:phosphorelay response regulator activity; IDA:CACAO.
DR GO; GO:0006355; P:regulation of transcription, DNA-templated; IMP:CACAO.
DR CDD; cd00383; trans_reg_C; 1.
DR Gene3D; 1.10.10.10; -; 1.
DR InterPro; IPR001867; OmpR/PhoB-type_DNA-bd.
DR InterPro; IPR016032; Sig_transdc_resp-reg_C-effctor.
DR InterPro; IPR036388; WH-like_DNA-bd_sf.
DR Pfam; PF00486; Trans_reg_C; 1.
DR SMART; SM00862; Trans_reg_C; 1.
DR SUPFAM; SSF46894; SSF46894; 1.
DR PROSITE; PS51755; OMPR_PHOB; 1.
PE 4: Predicted;
KW Activator; Cell membrane; DNA-binding; Membrane; Reference proteome;
KW Transcription; Transcription regulation; Transmembrane;
KW Transmembrane helix; Two-component regulatory system.
FT CHAIN 1..294
FT /note="Cholera toxin transcriptional activator"
FT /id="PRO_0000081346"
FT TOPO_DOM 1..182
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 183..198
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 199..294
FT /note="Periplasmic"
FT /evidence="ECO:0000255"
FT DNA_BIND 18..121
FT /note="OmpR/PhoB-type"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01091"
FT CONFLICT 134
FT /note="S -> N (in Ref. 1; AAA27549)"
FT /evidence="ECO:0000305"
FT CONFLICT 172
FT /note="T -> A (in Ref. 1; AAA27549)"
FT /evidence="ECO:0000305"
FT CONFLICT 184
FT /note="L -> F (in Ref. 1; AAA27549)"
FT /evidence="ECO:0000305"
FT CONFLICT 204
FT /note="T -> S (in Ref. 1; AAA27549)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 294 AA; 32506 MW; 15EF85EA653C373E CRC64;
MFGLGHNSKE ISMSHIGTKF ILAEKFTFDP LSNTLIDKED SEEIIRLGSN ESRILWLLAQ
RPNEVISRND LHDFVWREQG FEVDDSSLTQ AISTLRKMLK DSTKSPQYVK TVPKRGYQLI
ARVETVEEEM ARESEAAHDI SQPESVNEYA ESSSVPSSAT VVNTPQPANV VTNKSAPNLG
NRLLILIAVL LPLAVLLLTN PSQTSFKPLT VVDGVAVNMP NNHPDLSNWL PSIELCVKKY
NEKHTGGLKP IEVIATGGQN NQLTLNYIHS PEVSGENITL RIVANPNDAI KVCE
