TOZ_ARATH
ID TOZ_ARATH Reviewed; 876 AA.
AC Q9LFE2; Q42339; Q8L830; Q93ZT0;
DT 25-MAY-2022, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-2000, sequence version 1.
DT 03-AUG-2022, entry version 156.
DE RecName: Full=Protein TORMOZ EMBRYO DEFECTIVE {ECO:0000303|PubMed:17616738};
GN Name=TOZ {ECO:0000303|PubMed:17616738};
GN OrderedLocusNames=At5g16750 {ECO:0000312|Araport:AT5G16750};
GN ORFNames=F5E19.90 {ECO:0000312|EMBL:AED92333.1};
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=11130714; DOI=10.1038/35048507;
RA Tabata S., Kaneko T., Nakamura Y., Kotani H., Kato T., Asamizu E.,
RA Miyajima N., Sasamoto S., Kimura T., Hosouchi T., Kawashima K., Kohara M.,
RA Matsumoto M., Matsuno A., Muraki A., Nakayama S., Nakazaki N., Naruo K.,
RA Okumura S., Shinpo S., Takeuchi C., Wada T., Watanabe A., Yamada M.,
RA Yasuda M., Sato S., de la Bastide M., Huang E., Spiegel L., Gnoj L.,
RA O'Shaughnessy A., Preston R., Habermann K., Murray J., Johnson D.,
RA Rohlfing T., Nelson J., Stoneking T., Pepin K., Spieth J., Sekhon M.,
RA Armstrong J., Becker M., Belter E., Cordum H., Cordes M., Courtney L.,
RA Courtney W., Dante M., Du H., Edwards J., Fryman J., Haakensen B.,
RA Lamar E., Latreille P., Leonard S., Meyer R., Mulvaney E., Ozersky P.,
RA Riley A., Strowmatt C., Wagner-McPherson C., Wollam A., Yoakum M., Bell M.,
RA Dedhia N., Parnell L., Shah R., Rodriguez M., Hoon See L., Vil D.,
RA Baker J., Kirchoff K., Toth K., King L., Bahret A., Miller B., Marra M.A.,
RA Martienssen R., McCombie W.R., Wilson R.K., Murphy G., Bancroft I.,
RA Volckaert G., Wambutt R., Duesterhoeft A., Stiekema W., Pohl T.,
RA Entian K.-D., Terryn N., Hartley N., Bent E., Johnson S., Langham S.-A.,
RA McCullagh B., Robben J., Grymonprez B., Zimmermann W., Ramsperger U.,
RA Wedler H., Balke K., Wedler E., Peters S., van Staveren M., Dirkse W.,
RA Mooijman P., Klein Lankhorst R., Weitzenegger T., Bothe G., Rose M.,
RA Hauf J., Berneiser S., Hempel S., Feldpausch M., Lamberth S.,
RA Villarroel R., Gielen J., Ardiles W., Bents O., Lemcke K., Kolesov G.,
RA Mayer K.F.X., Rudd S., Schoof H., Schueller C., Zaccaria P., Mewes H.-W.,
RA Bevan M., Fransz P.F.;
RT "Sequence and analysis of chromosome 5 of the plant Arabidopsis thaliana.";
RL Nature 408:823-826(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RX PubMed=14593172; DOI=10.1126/science.1088305;
RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA Ecker J.R.;
RT "Empirical analysis of transcriptional activity in the Arabidopsis
RT genome.";
RL Science 302:842-846(2003).
RN [4]
RP NUCLEOTIDE SEQUENCE [MRNA] OF 183-276.
RC STRAIN=cv. Columbia; TISSUE=Protoplast;
RA Cooke R., Laudie M., Raynal M., Delseny M.;
RT "The Arabidopsis thaliana transcribed genome: the GDR cDNA program.";
RL Submitted (MAR-1996) to the EMBL/GenBank/DDBJ databases.
RN [5]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX PubMed=17272265; DOI=10.1074/mcp.m600408-mcp200;
RA Maor R., Jones A., Nuehse T.S., Studholme D.J., Peck S.C., Shirasu K.;
RT "Multidimensional protein identification technology (MudPIT) analysis of
RT ubiquitinated proteins in plants.";
RL Mol. Cell. Proteomics 6:601-610(2007).
RN [6]
RP FUNCTION, DISRUPTION PHENOTYPE, SUBCELLULAR LOCATION, TISSUE SPECIFICITY,
RP AND DEVELOPMENTAL STAGE.
RC STRAIN=cv. Landsberg erecta;
RX PubMed=17616738; DOI=10.1105/tpc.106.042697;
RA Griffith M.E., Mayer U., Capron A., Ngo Q.A., Surendrarao A., McClinton R.,
RA Juergens G., Sundaresan V.;
RT "The TORMOZ gene encodes a nucleolar protein required for regulated
RT division planes and embryo development in Arabidopsis.";
RL Plant Cell 19:2246-2263(2007).
RN [7]
RP REVIEW ON EMBRYO-DEFECTIVE MUTANTS.
RX PubMed=31334862; DOI=10.1111/nph.16071;
RA Meinke D.W.;
RT "Genome-wide identification of EMBRYO-DEFECTIVE (EMB) genes required for
RT growth and development in Arabidopsis.";
RL New Phytol. 226:306-325(2020).
CC -!- FUNCTION: Essential protein involved in the regulation of cell division
CC planes during embryogenesis which defines cell patterning, especially
CC longitudinal division planes of the proembryo, probably via the
CC regulation of embryo patterning genes expression patterns.
CC {ECO:0000269|PubMed:17616738}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000255|PROSITE-ProRule:PRU00768,
CC ECO:0000269|PubMed:17616738}. Nucleus, nucleolus
CC {ECO:0000269|PubMed:17616738}.
CC -!- TISSUE SPECIFICITY: Preferentially expressed in dividing cells in a
CC variety of tissues and meristematic regions.
CC {ECO:0000269|PubMed:17616738}.
CC -!- DEVELOPMENTAL STAGE: Expressed in young proliferating tissues, such as
CC root tips, meristems, young leaves and floral buds (PubMed:17616738).
CC Also observed in the mature embryo sac of the female gametophyte
CC (PubMed:17616738). In embryos, first observed at low levels very early
CC in the proembryo, accumulates strongly in embryos with more than eight
CC cells and remains at high levels until cell division ceased prior to
CC dormancy (PubMed:17616738). In seedlings, concentrated at the
CC developing apex in all root tips and later in the developing flowers of
CC the inflorescence (PubMed:17616738). {ECO:0000269|PubMed:17616738}.
CC -!- DISRUPTION PHENOTYPE: Defective embryo arrested at preglobular stage
CC characterized by aberrant cell division planes leading to an abnormal
CC cell patterning; longitudinal division planes of the proembryo are
CC frequently replaced by transverse divisions and less frequently by
CC oblique divisions (PubMed:17616738). Altered embryo patterning genes
CC expression patterns (PubMed:17616738). {ECO:0000269|PubMed:17616738}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAL07130.1; Type=Erroneous initiation; Note=Truncated N-terminus.; Evidence={ECO:0000305};
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DR EMBL; AL391147; CAC01839.1; -; Genomic_DNA.
DR EMBL; CP002688; AED92333.1; -; Genomic_DNA.
DR EMBL; AY056281; AAL07130.1; ALT_INIT; mRNA.
DR EMBL; AY117219; AAM51294.1; -; mRNA.
DR EMBL; AY120781; AAM53339.1; -; mRNA.
DR EMBL; F19990; CAA23372.1; -; mRNA.
DR PIR; T51507; T51507.
DR RefSeq; NP_568338.2; NM_121681.4.
DR AlphaFoldDB; Q9LFE2; -.
DR SMR; Q9LFE2; -.
DR STRING; 3702.AT5G16750.1; -.
DR PaxDb; Q9LFE2; -.
DR PRIDE; Q9LFE2; -.
DR ProteomicsDB; 181103; -.
DR EnsemblPlants; AT5G16750.1; AT5G16750.1; AT5G16750.
DR GeneID; 831538; -.
DR Gramene; AT5G16750.1; AT5G16750.1; AT5G16750.
DR KEGG; ath:AT5G16750; -.
DR Araport; AT5G16750; -.
DR TAIR; locus:2148965; AT5G16750.
DR eggNOG; KOG0319; Eukaryota.
DR HOGENOM; CLU_009276_0_0_1; -.
DR InParanoid; Q9LFE2; -.
DR OMA; PYVQRHF; -.
DR OrthoDB; 1112002at2759; -.
DR PhylomeDB; Q9LFE2; -.
DR Proteomes; UP000006548; Chromosome 5.
DR ExpressionAtlas; Q9LFE2; baseline and differential.
DR GO; GO:0030686; C:90S preribosome; IBA:GO_Central.
DR GO; GO:0005730; C:nucleolus; IDA:TAIR.
DR GO; GO:0032040; C:small-subunit processome; IEA:InterPro.
DR GO; GO:0034511; F:U3 snoRNA binding; IBA:GO_Central.
DR GO; GO:0051301; P:cell division; IMP:TAIR.
DR GO; GO:0009793; P:embryo development ending in seed dormancy; IMP:TAIR.
DR GO; GO:0009880; P:embryonic pattern specification; IMP:TAIR.
DR GO; GO:0000480; P:endonucleolytic cleavage in 5'-ETS of tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA); IBA:GO_Central.
DR GO; GO:0000472; P:endonucleolytic cleavage to generate mature 5'-end of SSU-rRNA from (SSU-rRNA, 5.8S rRNA, LSU-rRNA); IBA:GO_Central.
DR Gene3D; 2.130.10.10; -; 4.
DR InterPro; IPR020472; G-protein_beta_WD-40_rep.
DR InterPro; IPR011047; Quinoprotein_ADH-like_supfam.
DR InterPro; IPR013934; SSU_processome_Utp13.
DR InterPro; IPR015943; WD40/YVTN_repeat-like_dom_sf.
DR InterPro; IPR001680; WD40_repeat.
DR InterPro; IPR019775; WD40_repeat_CS.
DR InterPro; IPR036322; WD40_repeat_dom_sf.
DR Pfam; PF08625; Utp13; 1.
DR Pfam; PF00400; WD40; 10.
DR PRINTS; PR00320; GPROTEINBRPT.
DR SMART; SM00320; WD40; 11.
DR SUPFAM; SSF50978; SSF50978; 1.
DR SUPFAM; SSF50998; SSF50998; 1.
DR PROSITE; PS00678; WD_REPEATS_1; 4.
DR PROSITE; PS50082; WD_REPEATS_2; 10.
PE 1: Evidence at protein level;
KW Nucleus; Reference proteome; Repeat; WD repeat.
FT CHAIN 1..876
FT /note="Protein TORMOZ EMBRYO DEFECTIVE"
FT /id="PRO_0000455301"
FT REPEAT 58..97
FT /note="WD 1"
FT /evidence="ECO:0000255"
FT REPEAT 100..139
FT /note="WD 2"
FT /evidence="ECO:0000255"
FT REPEAT 142..183
FT /note="WD 3"
FT /evidence="ECO:0000255"
FT REPEAT 190..229
FT /note="WD 4"
FT /evidence="ECO:0000255"
FT REPEAT 255..294
FT /note="WD 5"
FT /evidence="ECO:0000255"
FT REPEAT 308..347
FT /note="WD 6"
FT /evidence="ECO:0000255"
FT REPEAT 356..396
FT /note="WD 7"
FT /evidence="ECO:0000255"
FT REPEAT 399..441
FT /note="WD 8"
FT /evidence="ECO:0000255"
FT REPEAT 444..484
FT /note="WD 9"
FT /evidence="ECO:0000255"
FT REPEAT 497..536
FT /note="WD 10"
FT /evidence="ECO:0000255"
FT REPEAT 539..580
FT /note="WD 11"
FT /evidence="ECO:0000255"
FT REPEAT 581..620
FT /note="WD 12"
FT /evidence="ECO:0000255"
FT REPEAT 623..662
FT /note="WD 13"
FT /evidence="ECO:0000255"
FT REGION 816..876
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOTIF 848..855
FT /note="Nuclear localization signal"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00768"
FT COMPBIAS 816..851
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 876 AA; 96698 MW; AFC123DAEB48F4E8 CRC64;
MAPHSLKKNY RCSRSLKQFY GGGPFIVSSD GSFIACACGD VINIVDSTDS SVKSTIEGES
DTLTALALSP DDKLLFSAGH SRQIRVWDLE TLKCIRSWKG HEGPVMGMAC HASGGLLATA
GADRKVLVWD VDGGFCTHYF RGHKGVVSSI LFHPDSNKNI LISGSDDATV RVWDLNAKNT
EKKCLAIMEK HFSAVTSIAL SEDGLTLFSA GRDKVVNLWD LHDYSCKATV ATYEVLEAVT
TVSSGTPFAS FVASLDQKKS KKKESDSQAT YFITVGERGV VRIWKSEGSI CLYEQKSSDI
TVSSDDEESK RGFTAAAMLP SDHGLLCVTA DQQFFFYSVV ENVEETELVL SKRLVGYNEE
IADMKFLGDE EQFLAVATNL EEVRVYDVAT MSCSYVLAGH KEVVLSLDTC VSSSGNVLIV
TGSKDKTVRL WNATSKSCIG VGTGHNGDIL AVAFAKKSFS FFVSGSGDRT LKVWSLDGIS
EDSEEPINLK TRSVVAAHDK DINSVAVARN DSLVCTGSED RTASIWRLPD LVHVVTLKGH
KRRIFSVEFS TVDQCVMTAS GDKTVKIWAI SDGSCLKTFE GHTSSVLRAS FITDGTQFVS
CGADGLLKLW NVNTSECIAT YDQHEDKVWA LAVGKKTEMI ATGGGDAVIN LWHDSTASDK
EDDFRKEEEA ILRGQELENA VLDAEYTKAI RLAFELCRPH KVFELFSGLC RKRDSDEQIV
KALQGLEKEE FRLLFEYVRE WNTKPKLCHI AQFVLYKTFN ILPPTEIVQV KGIGELLEGL
IPYSQRHFSR IDRFVRSSFL LDYTLGEMSV IDPETVETEY PKDEKKKEKD VIAAMEQDTD
ELKQETPSRK RKSQKSKGKS NKKRLIAEAQ GSVIAV
