TP1_ODOHA
ID TP1_ODOHA Reviewed; 62 AA.
AC E7EKD0;
DT 18-SEP-2013, integrated into UniProtKB/Swiss-Prot.
DT 08-MAR-2011, sequence version 1.
DT 25-MAY-2022, entry version 19.
DE RecName: Full=Temporin-HN1 {ECO:0000312|EMBL:ADV36130.1};
DE Flags: Precursor;
OS Odorrana hainanensis (Odor frog) (Rana hainanensis).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Amphibia;
OC Batrachia; Anura; Neobatrachia; Ranoidea; Ranidae; Odorrana.
OX NCBI_TaxID=431935;
RN [1] {ECO:0000305, ECO:0000312|EMBL:ADV36130.1}
RP NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 47-60, SYNTHESIS OF 47-60,
RP FUNCTION, SUBCELLULAR LOCATION, MASS SPECTROMETRY, AND AMIDATION AT LEU-60.
RC TISSUE=Skin {ECO:0000269|PubMed:22450466}, and
RC Skin secretion {ECO:0000269|PubMed:22450466};
RX PubMed=22450466; DOI=10.1016/j.peptides.2012.03.007;
RA Wang H., Yu Z., Hu Y., Li F., Liu L., Zheng H., Meng H., Yang S., Yang X.,
RA Liu J.;
RT "Novel antimicrobial peptides isolated from the skin secretions of Hainan
RT odorous frog, Odorrana hainanensis.";
RL Peptides 35:285-290(2012).
CC -!- FUNCTION: Has antimicrobial activity against some Gram-positive
CC bacteria and fungi but has no activity against a range of Gram-negative
CC bacteria except P.faecalis. Active against the Gram-positive bacteria
CC S.aureus ATCC 25923 (MIC=37.5 uM), S.carnosus KHS (MIC=37.5 uM),
CC B.licheniformis X39 (MIC=19 uM), R.rhodochrous X15 (MIC=4.8 uM), is
CC virtually inactive against E.faecalis 981 (MIC=150 uM) and inactive
CC against E.faecium 091299. Has some antimicrobial activity against the
CC Gram-negative bacterium P.faecalis X29 (MIC=75 uM) and is inactive
CC against E.coli, P.aeruginosa and S.typhi. Has antifungal activity
CC against C.albicans ATCC 2002 (MIC=19 uM) and lower activity against the
CC slime mold 090223 (MIC=75 uM). Has low hemolytic activity against human
CC erythrocytes (LC(50)=75 uM). {ECO:0000269|PubMed:22450466}.
CC -!- SUBCELLULAR LOCATION: Secreted {ECO:0000269|PubMed:22450466}.
CC -!- TISSUE SPECIFICITY: Expressed by the skin glands.
CC {ECO:0000305|PubMed:22450466}.
CC -!- MASS SPECTROMETRY: Mass=1617.00; Method=Electrospray;
CC Evidence={ECO:0000269|PubMed:22450466};
CC -!- SIMILARITY: Belongs to the frog skin active peptide (FSAP) family.
CC Temporin subfamily. {ECO:0000255}.
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DR EMBL; HQ735107; ADV36130.1; -; mRNA.
DR AlphaFoldDB; E7EKD0; -.
DR GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR GO; GO:0042742; P:defense response to bacterium; IEA:UniProtKB-KW.
DR GO; GO:0050832; P:defense response to fungus; IEA:UniProtKB-KW.
DR GO; GO:0044179; P:hemolysis in another organism; IEA:UniProtKB-KW.
DR InterPro; IPR004275; Frog_antimicrobial_propeptide.
DR Pfam; PF03032; FSAP_sig_propep; 1.
PE 1: Evidence at protein level;
KW Amidation; Amphibian defense peptide; Antibiotic; Antimicrobial;
KW Cleavage on pair of basic residues; Cytolysis; Direct protein sequencing;
KW Fungicide; Hemolysis; Secreted; Signal.
FT SIGNAL 1..22
FT /evidence="ECO:0000255"
FT PROPEP 23..44
FT /evidence="ECO:0000255, ECO:0000269|PubMed:22450466"
FT /id="PRO_0000423525"
FT PEPTIDE 47..60
FT /note="Temporin-HN1"
FT /evidence="ECO:0000269|PubMed:22450466"
FT /id="PRO_0000423526"
FT MOD_RES 60
FT /note="Leucine amide"
FT /evidence="ECO:0000269|PubMed:22450466"
SQ SEQUENCE 62 AA; 7223 MW; D9B4C6357BD7EDF3 CRC64;
MFTLKKSLLL LLFLGTINLS LSEQERNAEE ERRDDPEEMD AEVEKRAILT TLANWARKFL
GK