TP326_TREPA
ID TP326_TREPA Reviewed; 837 AA.
AC O83346;
DT 09-DEC-2015, integrated into UniProtKB/Swiss-Prot.
DT 09-DEC-2015, sequence version 2.
DT 25-MAY-2022, entry version 114.
DE RecName: Full=Putative outer membrane protein assembly factor TP_0326 {ECO:0000305|PubMed:21488980};
DE AltName: Full=TP_92 {ECO:0000303|PubMed:12904373};
DE Flags: Precursor;
GN Name=tp92; OrderedLocusNames=TP_0326; ORFNames=TPANIC_0326;
OS Treponema pallidum (strain Nichols).
OC Bacteria; Spirochaetes; Spirochaetales; Treponemataceae; Treponema.
OX NCBI_TaxID=243276;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Nichols;
RX PubMed=9665876; DOI=10.1126/science.281.5375.375;
RA Fraser C.M., Norris S.J., Weinstock G.M., White O., Sutton G.G.,
RA Dodson R.J., Gwinn M.L., Hickey E.K., Clayton R.A., Ketchum K.A.,
RA Sodergren E., Hardham J.M., McLeod M.P., Salzberg S.L., Peterson J.D.,
RA Khalak H.G., Richardson D.L., Howell J.K., Chidambaram M., Utterback T.R.,
RA McDonald L.A., Artiach P., Bowman C., Cotton M.D., Fujii C., Garland S.A.,
RA Hatch B., Horst K., Roberts K.M., Sandusky M., Weidman J.F., Smith H.O.,
RA Venter J.C.;
RT "Complete genome sequence of Treponema pallidum, the syphilis spirochete.";
RL Science 281:375-388(1998).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Nichols;
RX PubMed=24058545; DOI=10.1371/journal.pone.0074319;
RA Petrosova H., Pospisilova P., Strouhal M., Cejkova D., Zobanikova M.,
RA Mikalova L., Sodergren E., Weinstock G.M., Smajs D.;
RT "Resequencing of Treponema pallidum ssp. pallidum strains Nichols and SS14:
RT correction of sequencing errors resulted in increased separation of
RT syphilis treponeme subclusters.";
RL PLoS ONE 8:E74319-E74319(2013).
RN [3]
RP BIOTECHNOLOGY.
RX PubMed=12904373; DOI=10.1128/jcm.41.8.3668-3674.2003;
RA Van Voorhis W.C., Barrett L.K., Lukehart S.A., Schmidt B., Schriefer M.,
RA Cameron C.E.;
RT "Serodiagnosis of syphilis: antibodies to recombinant Tp0453, Tp92, and Gpd
RT proteins are sensitive and specific indicators of infection by Treponema
RT pallidum.";
RL J. Clin. Microbiol. 41:3668-3674(2003).
RN [4]
RP FUNCTION IN HOST IMMUNE SYSTEM RESPONSE, SUBUNIT, SUBCELLULAR LOCATION,
RP INDUCTION, DOMAIN, AND TOPOLOGY.
RC STRAIN=Nichols-Farmington;
RX PubMed=21488980; DOI=10.1111/j.1365-2958.2011.07662.x;
RA Desrosiers D.C., Anand A., Luthra A., Dunham-Ems S.M., LeDoyt M.,
RA Cummings M.A., Eshghi A., Cameron C.E., Cruz A.R., Salazar J.C.,
RA Caimano M.J., Radolf J.D.;
RT "TP0326, a Treponema pallidum beta-barrel assembly machinery A (BamA)
RT orthologue and rare outer membrane protein.";
RL Mol. Microbiol. 80:1496-1515(2011).
RN [5]
RP BIOTECHNOLOGY.
RC STRAIN=Nichols;
RX PubMed=23100335; DOI=10.1128/jcm.01390-12;
RA Smith B.C., Simpson Y., Morshed M.G., Cowen L.L., Hof R., Wetherell C.,
RA Cameron C.E.;
RT "New proteins for a new perspective on syphilis diagnosis.";
RL J. Clin. Microbiol. 51:105-111(2013).
RN [6]
RP FUNCTION IN HOST IMMUNE SYSTEM RESPONSE, SUBCELLULAR LOCATION, STRUCTURE
RP MODEL, TOPOLOGY, AND EXPRESSION IN E.COLI.
RC STRAIN=Nichols-Farmington;
RX PubMed=25825429; DOI=10.1128/jb.00086-15;
RA Luthra A., Anand A., Hawley K.L., LeDoyt M., La Vake C.J., Caimano M.J.,
RA Cruz A.R., Salazar J.C., Radolf J.D.;
RT "A homology model reveals novel structural features and an immunodominant
RT surface loop/opsonic target in the Treponema pallidum BamA ortholog
RT TP_0326.";
RL J. Bacteriol. 197:1906-1920(2015).
CC -!- FUNCTION: Might be part of the outer membrane protein assembly complex,
CC which is involved in assembly and insertion of beta-barrel proteins
CC into the outer membrane. {ECO:0000250|UniProtKB:P0A940}.
CC -!- FUNCTION: Both rabbit immune serum and rabbit antiserum specific for
CC extracytoplasmic loop L4 promote bacteria internalization by rabbit
CC peritoneal macrophages. Pools of human syphilitic sera from the USA and
CC Columbia recognize both the N-terminal POTRA-containing and C-terminal
CC beta-barrel domains as well as loop L4, showing this protein stimulates
CC the immune system in both humans and rabbits (PubMed:25825429).
CC {ECO:0000269|PubMed:21488980, ECO:0000269|PubMed:25825429}.
CC -!- SUBUNIT: Part of 2 complexes of about 239 and 164 kDa.
CC {ECO:0000269|PubMed:21488980}.
CC -!- SUBCELLULAR LOCATION: Cell outer membrane {ECO:0000269|PubMed:21488980,
CC ECO:0000269|PubMed:25825429}. Note=The C-terminal beta-barrel domain is
CC exposed on the outer surface of the outer membrane (PubMed:21488980).
CC Antibody labeling by antisera to either the periplasmic or membrane
CC domain occurs in a punctate fashion over the length of the spirochete;
CC detection by periplasmic POTRA-domain-specific antibodies only occurs
CC after mild detergent solubilization (PubMed:25825429).
CC {ECO:0000269|PubMed:21488980, ECO:0000269|PubMed:25825429}.
CC -!- INDUCTION: Transcribed at low levels, estimated to be present at about
CC 100 molecules per cell (at protein level).
CC {ECO:0000269|PubMed:21488980}.
CC -!- DOMAIN: The N-terminus (residues 22-369) partitions into an aqueous
CC phase and is predicted to be periplasmic, while the C-terminus
CC (residues 426-837) partitions into Triton X-114 and has the hallmarks
CC of a beta-stranded protein, suggesting it is membraneous.
CC {ECO:0000269|PubMed:21488980}.
CC -!- BIOTECHNOLOGY: Recognized by sera from 42/43 syphilis patients, it
CC shows promise as a diagnostic antigen (PubMed:12904373). In further
CC diagnostic tests for syphilis this protein exhibits a sensitivity of
CC 86% and a specificity of 99%. A fusion with TP0453 (residues 32-287 of
CC TP0453 fused N-terminally to residues 38-450 of TP0326) exhibits a
CC sensitivity of 98% and a specificity of 99%, and exhibits superior
CC accuracy in classifying analytical false-positive samples than a
CC commericially available test, making it a better candiate for diagnosis
CC (PubMed:23100335). {ECO:0000269|PubMed:12904373,
CC ECO:0000269|PubMed:23100335}.
CC -!- MISCELLANEOUS: The outer membrane of T.pallidum has no
CC lipopolysaccharides, few proteins and is a fluid and very fragile
CC bilayer. Its lack of surface antigenicity is thought to contribute to
CC the ability of the pathogen to evade the human immune system.
CC {ECO:0000305}.
CC -!- SIMILARITY: Belongs to the BamA family. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAC65313.1; Type=Erroneous initiation; Note=Extended N-terminus.; Evidence={ECO:0000305};
CC Sequence=AGN75525.1; Type=Erroneous initiation; Note=Extended N-terminus.; Evidence={ECO:0000305};
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DR EMBL; AE000520; AAC65313.1; ALT_INIT; Genomic_DNA.
DR EMBL; CP004010; AGN75525.1; ALT_INIT; Genomic_DNA.
DR PIR; A71339; A71339.
DR RefSeq; WP_014342788.1; NC_021490.2.
DR AlphaFoldDB; O83346; -.
DR SMR; O83346; -.
DR STRING; 243276.TPANIC_0326; -.
DR EnsemblBacteria; AAC65313; AAC65313; TP_0326.
DR EnsemblBacteria; AGN75525; AGN75525; TPANIC_0326.
DR KEGG; tpa:TP_0326; -.
DR KEGG; tpw:TPANIC_0326; -.
DR PATRIC; fig|243276.9.peg.324; -.
DR eggNOG; COG4775; Bacteria.
DR HOGENOM; CLU_007664_1_1_12; -.
DR OrthoDB; 258122at2; -.
DR Proteomes; UP000000811; Chromosome.
DR GO; GO:0009279; C:cell outer membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0071709; P:membrane assembly; IEA:InterPro.
DR InterPro; IPR000184; Bac_surfAg_D15.
DR InterPro; IPR010827; BamA/TamA_POTRA.
DR InterPro; IPR039910; D15-like.
DR InterPro; IPR023707; OM_assembly_BamA.
DR InterPro; IPR034746; POTRA.
DR PANTHER; PTHR12815; PTHR12815; 1.
DR PANTHER; PTHR12815:SF23; PTHR12815:SF23; 1.
DR Pfam; PF01103; Omp85; 1.
DR Pfam; PF07244; POTRA; 4.
DR PIRSF; PIRSF006076; OM_assembly_OMP85; 1.
DR TIGRFAMs; TIGR03303; OM_YaeT; 1.
DR PROSITE; PS51779; POTRA; 5.
PE 1: Evidence at protein level;
KW Cell outer membrane; Membrane; Reference proteome; Repeat; Signal;
KW Transmembrane; Transmembrane beta strand.
FT SIGNAL 1..21
FT /evidence="ECO:0000255"
FT CHAIN 22..837
FT /note="Putative outer membrane protein assembly factor
FT TP_0326"
FT /id="PRO_5004161024"
FT TOPO_DOM 22..433
FT /note="Periplasmic"
FT /evidence="ECO:0000305"
FT TRANSMEM 434..442
FT /note="Beta stranded; Name=1"
FT /evidence="ECO:0000303|PubMed:25825429"
FT TOPO_DOM 443..450
FT /note="Extracellular; loop L1"
FT /evidence="ECO:0000305"
FT TRANSMEM 451..461
FT /note="Beta stranded; Name=2"
FT /evidence="ECO:0000303|PubMed:25825429"
FT TOPO_DOM 462..468
FT /note="Periplasmic"
FT /evidence="ECO:0000305"
FT TRANSMEM 469..476
FT /note="Beta stranded; Name=3"
FT /evidence="ECO:0000303|PubMed:25825429"
FT TOPO_DOM 477..478
FT /note="Extracellular; loop L2"
FT /evidence="ECO:0000305"
FT TRANSMEM 479..489
FT /note="Beta stranded; Name=4"
FT /evidence="ECO:0000303|PubMed:25825429"
FT TOPO_DOM 490..499
FT /note="Periplasmic"
FT /evidence="ECO:0000305"
FT TRANSMEM 500..520
FT /note="Beta stranded; Name=5"
FT /evidence="ECO:0000303|PubMed:25825429"
FT TOPO_DOM 521..530
FT /note="Extracellular; loop L3"
FT /evidence="ECO:0000305"
FT TRANSMEM 531..543
FT /note="Beta stranded; Name=6"
FT /evidence="ECO:0000303|PubMed:25825429"
FT TOPO_DOM 544..554
FT /note="Periplasmic"
FT /evidence="ECO:0000305"
FT TRANSMEM 555..568
FT /note="Beta stranded; Name=7"
FT /evidence="ECO:0000303|PubMed:25825429"
FT TOPO_DOM 569..601
FT /note="Extracellular; loop L4"
FT /evidence="ECO:0000305|PubMed:25825429"
FT TRANSMEM 602..615
FT /note="Beta stranded; Name=8"
FT /evidence="ECO:0000303|PubMed:25825429"
FT TOPO_DOM 616..623
FT /note="Periplasmic"
FT /evidence="ECO:0000305"
FT TRANSMEM 624..636
FT /note="Beta stranded; Name=9"
FT /evidence="ECO:0000303|PubMed:25825429"
FT TOPO_DOM 637..644
FT /note="Extracellular; loop L5"
FT /evidence="ECO:0000305"
FT TRANSMEM 645..658
FT /note="Beta stranded; Name=10"
FT /evidence="ECO:0000303|PubMed:25825429"
FT TOPO_DOM 659..667
FT /note="Periplasmic"
FT /evidence="ECO:0000305"
FT TRANSMEM 668..682
FT /note="Beta stranded; Name=11"
FT /evidence="ECO:0000303|PubMed:25825429"
FT TOPO_DOM 683..724
FT /note="Extracellular; loop L6"
FT /evidence="ECO:0000305"
FT TRANSMEM 725..736
FT /note="Beta stranded; Name=12"
FT /evidence="ECO:0000303|PubMed:25825429"
FT TOPO_DOM 737..741
FT /note="Periplasmic"
FT /evidence="ECO:0000305"
FT TRANSMEM 742..756
FT /note="Beta stranded; Name=13"
FT /evidence="ECO:0000303|PubMed:25825429"
FT TOPO_DOM 757..786
FT /note="Extracellular; loop L7"
FT /evidence="ECO:0000305"
FT TRANSMEM 787..799
FT /note="Beta stranded; Name=14"
FT /evidence="ECO:0000303|PubMed:25825429"
FT TOPO_DOM 800..802
FT /note="Periplasmic"
FT /evidence="ECO:0000305"
FT TRANSMEM 803..814
FT /note="Beta stranded; Name=15"
FT /evidence="ECO:0000303|PubMed:25825429"
FT TOPO_DOM 815..824
FT /note="Extracellular; loop L8"
FT /evidence="ECO:0000305"
FT TRANSMEM 825..829
FT /note="Beta stranded; Name=16"
FT /evidence="ECO:0000303|PubMed:25825429"
FT TOPO_DOM 830..837
FT /note="Periplasmic"
FT /evidence="ECO:0000305"
FT DOMAIN 31..105
FT /note="POTRA 1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01115,
FT ECO:0000305|PubMed:21488980"
FT DOMAIN 106..182
FT /note="POTRA 2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01115"
FT DOMAIN 185..273
FT /note="POTRA 3"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01115"
FT DOMAIN 276..354
FT /note="POTRA 4"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01115"
FT DOMAIN 357..430
FT /note="POTRA 5"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01115"
FT REGION 761..785
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 837 AA; 94267 MW; 10DA39487F828FBC CRC64;
MLKKASAFLI ASCCVMSLAW AQANDNWYEG KPISAISFEG LEYIARGQLD TIFSQYKGQK
WTYELYLEIL QKVYDLEYFS EVSPKAVPTD PEYQYVMLQF TVKERPSVKG IKMVGNSQIR
SGDLLSKILL KKGDIYNEVK MKVDQESLRR HYLDQGYAAV KISCEAKTEA GGVVVQFTIQ
EGKQTVVSRI QFKGNKAFTE SVLKKVLSTQ EARFLTSGVF KENALEADKA AVHSYYAERG
YIDARVEGVA KTVDKKTDAS RNLVTLTYTV VEGEQYRYGG VTIVGNQIFS TEELQAKIRL
KRGAIMNMVA FEQGFQALAD AYFENGYTSN YLNKEEHRDT AEKTLSFKIT VVERERSHVE
HIIIKGTKNT KDEVILREML LKPGDVFSKS KFTDSLRNLF NLRYFSSLVP DVRPGSEQDL
VDIILNVEEQ STANVQFGVT FSGVGEAGTF PLSLFCQWEE KNFLGKGNEI SVNATLGSEA
QSLKLGYVER WFLGSPLTVG FDFELTHKNL FVYRAGSYGN GLPHPYTSRE QWASSPGLAE
SFRLKYSRFE SAIGAHTGYQ WYPRYAVIRV NGGVDFRVVK NFYDKDNNQP FDLTVKEQLN
WTSINSFWTS VSFDGRDFAY DPSSGWFLGQ RCTFNGLVPF LEKEHSFRSD TKAEFYVTLL
NYPVSAVWNL KFVLAFYTGV SVQTYYGRRK SENGKGNGVR SGALVIDGVL VGRGWSEDAK
KNTGDLLLHH WIEFRWPLAH GIVSFDFFFD AAMVYNIESQ SPNGSSSASS SSSSSSSSSR
TTSSEGLYKM SYGPGLRFTL PQFPLKLAFA NTFTSPGGIP KTKKNWNFVL SFTVNNL
