ID   TPAMO_AMOLO             Reviewed;          16 AA.
AC   P0DTU0;
DT   17-JUN-2020, integrated into UniProtKB/Swiss-Prot.
DT   17-JUN-2020, sequence version 1.
DT   25-MAY-2022, entry version 3.
DE   RecName: Full=Temporin {ECO:0000305};
DE   AltName: Full=Amolopin {ECO:0000303|PubMed:25294221};
OS   Amolops loloensis (Lolokou Sucker Frog) (Staurois loloensis).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Amphibia;
OC   Batrachia; Anura; Neobatrachia; Ranoidea; Ranidae; Amolops.
OX   NCBI_TaxID=318551;
RN   [1]
RP   PROTEIN SEQUENCE, SUBCELLULAR LOCATION, MASS SPECTROMETRY, AMIDATION AT
RP   LEU-16, AND PHARMACEUTICAL.
RC   TISSUE=Skin secretion;
RX   PubMed=25294221; DOI=10.1007/s13659-014-0037-z;
RA   Mo G.X., Bai X.W., Li Z.J., Yan X.W., He X.Q., Rong M.Q.;
RT   "A novel insulinotropic peptide from the skin secretions of Amolops
RT   loloensis frog.";
RL   Nat. Prod. Bioprospect. 4:309-313(2014).
CC   -!- FUNCTION: Probable antimicrobial peptide that can stimulate insulin
CC       release in INS-1 cells in a dose-dependent manner and without
CC       increasing the influx of calcium. {ECO:0000269|PubMed:25294221}.
CC   -!- SUBCELLULAR LOCATION: Secreted {ECO:0000269|PubMed:25294221}.
CC   -!- TISSUE SPECIFICITY: Expressed by the skin glands.
CC       {ECO:0000305|PubMed:25294221}.
CC   -!- MASS SPECTROMETRY: Mass=1615.5; Method=FAB;
CC       Evidence={ECO:0000269|PubMed:25294221};
CC   -!- PHARMACEUTICAL: promising candidate to treat diabetes.
CC       {ECO:0000305|PubMed:25294221}.
CC   -!- SIMILARITY: Belongs to the frog skin active peptide (FSAP) family.
CC       Temporin subfamily. {ECO:0000305}.
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DR   AlphaFoldDB; P0DTU0; -.
DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR   GO; GO:0045087; P:innate immune response; IEA:UniProtKB-KW.
PE   1: Evidence at protein level;
KW   Amidation; Amphibian defense peptide; Antimicrobial;
KW   Direct protein sequencing; Immunity; Innate immunity; Pharmaceutical;
KW   Secreted.
FT   PEPTIDE         1..16
FT                   /note="Temporin"
FT                   /evidence="ECO:0000269|PubMed:25294221"
FT                   /id="PRO_0000450000"
FT   MOD_RES         16
FT                   /note="Leucine amide"
FT                   /evidence="ECO:0000269|PubMed:25294221"
SQ   SEQUENCE   16 AA;  1616 MW;  EA84C8BC63402B9D CRC64;
     FLPIVGKSLS GLSGKL