ID   BTUB_VIBC1              Reviewed;         617 AA.
AC   A7MXY8;
DT   05-FEB-2008, integrated into UniProtKB/Swiss-Prot.
DT   02-OCT-2007, sequence version 1.
DT   03-AUG-2022, entry version 93.
DE   RecName: Full=Vitamin B12 transporter BtuB {ECO:0000255|HAMAP-Rule:MF_01531};
DE   AltName: Full=Cobalamin receptor {ECO:0000255|HAMAP-Rule:MF_01531};
DE   AltName: Full=Outer membrane cobalamin translocator {ECO:0000255|HAMAP-Rule:MF_01531};
DE   Flags: Precursor;
GN   Name=btuB {ECO:0000255|HAMAP-Rule:MF_01531};
GN   OrderedLocusNames=VIBHAR_00261;
OS   Vibrio campbellii (strain ATCC BAA-1116).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Vibrionales; Vibrionaceae;
OC   Vibrio.
OX   NCBI_TaxID=2902295;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC BAA-1116 / BB120;
RG   The Vibrio harveyi Genome Sequencing Project;
RA   Bassler B., Clifton S.W., Fulton L., Delehaunty K., Fronick C.,
RA   Harrison M., Markivic C., Fulton R., Tin-Wollam A.-M., Shah N., Pepin K.,
RA   Nash W., Thiruvilangam P., Bhonagiri V., Waters C., Tu K.C., Irgon J.,
RA   Wilson R.K.;
RL   Submitted (AUG-2007) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: Involved in the active translocation of vitamin B12
CC       (cyanocobalamin) across the outer membrane to the periplasmic space. It
CC       derives its energy for transport by interacting with the trans-
CC       periplasmic membrane protein TonB. {ECO:0000255|HAMAP-Rule:MF_01531}.
CC   -!- SUBCELLULAR LOCATION: Cell outer membrane {ECO:0000255|HAMAP-
CC       Rule:MF_01531}; Multi-pass membrane protein {ECO:0000255|HAMAP-
CC       Rule:MF_01531}.
CC   -!- SIMILARITY: Belongs to the TonB-dependent receptor family. BtuB (TC
CC       1.B.14.3.1) subfamily. {ECO:0000255|HAMAP-Rule:MF_01531}.
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DR   EMBL; CP000789; ABU69289.1; -; Genomic_DNA.
DR   RefSeq; WP_012126562.1; NC_022269.1.
DR   AlphaFoldDB; A7MXY8; -.
DR   SMR; A7MXY8; -.
DR   EnsemblBacteria; ABU69289; ABU69289; VIBHAR_00261.
DR   KEGG; vha:VIBHAR_00261; -.
DR   PATRIC; fig|338187.25.peg.2308; -.
DR   OMA; SYELQWR; -.
DR   OrthoDB; 71288at2; -.
DR   Proteomes; UP000008152; Chromosome I.
DR   GO; GO:0009279; C:cell outer membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0046930; C:pore complex; IEA:UniProtKB-KW.
DR   GO; GO:0015420; F:ABC-type vitamin B12 transporter activity; IEA:InterPro.
DR   GO; GO:0015288; F:porin activity; IEA:UniProtKB-KW.
DR   GO; GO:0006811; P:ion transport; IEA:UniProtKB-KW.
DR   Gene3D; 2.170.130.10; -; 1.
DR   Gene3D; 2.40.170.20; -; 1.
DR   HAMAP; MF_01531; BtuB; 1.
DR   InterPro; IPR010101; B12_transptr_BtuB.
DR   InterPro; IPR039426; BtuB-like.
DR   InterPro; IPR012910; Plug_dom.
DR   InterPro; IPR037066; Plug_dom_sf.
DR   InterPro; IPR000531; TonB-dep_rcpt_b-brl.
DR   InterPro; IPR010916; TonB_box_CS.
DR   InterPro; IPR036942; TonB_rcpt_b-brl_sf.
DR   PANTHER; PTHR30069; PTHR30069; 1.
DR   Pfam; PF07715; Plug; 1.
DR   Pfam; PF00593; TonB_dep_Rec; 1.
DR   PROSITE; PS00430; TONB_DEPENDENT_REC_1; 1.
PE   3: Inferred from homology;
KW   Cell outer membrane; Ion transport; Membrane; Porin; Signal; TonB box;
KW   Transmembrane; Transmembrane beta strand; Transport.
FT   SIGNAL          1..22
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01531"
FT   CHAIN           23..617
FT                   /note="Vitamin B12 transporter BtuB"
FT                   /id="PRO_1000068688"
FT   MOTIF           29..36
FT                   /note="TonB box"
FT   MOTIF           600..617
FT                   /note="TonB C-terminal box"
SQ   SEQUENCE   617 AA;  68800 MW;  6D56F4D64A0E6102 CRC64;
     MRKTFLAITC ASLLSPTFYS QAQEVSADET VVVTANRFKQ IDGAVLAQTV TVTKEDIKRL
     QANSLFDVFR TLPSVEVAQY GGRGQSASIY VRGGSSSQVL VLVDGVRMPR AIMGGIDFNQ
     FPINSVERID YIRGARASIY GSEAISGVIN IITRAPINDD SGRVSAGYGS NNHKKGTFVV
     SKPVGEGKHF KGVLGYEKTD GFNVKPQPGL NDGDEHGFET LNLKLGYQQN FSDNLSGYVG
     VSAYNNEYDY DNSSIWSGHE KKTGEVEYVG TDLSLEYNKD VYSSELKLAY GQQDNYDHKS
     GQNKSTGDHV AIEQFNAIWL NSYSVNEELS IGGGLDYRTE KLAKGYIAPS DRGPAKDYDP
     EKNPRTNFGV SAIAQYAYDV WTLEASVRND DNNQFGNNTT WQTAAGWSFY EGYELTLSHG
     TAFRAPSFVD LYYPGYEMPN LKPEESENTE LSLSGAVSIV DWTVTGYYNE IENMLIWEGA
     GMQNVGEAEI KGVELEVKLD TDIVSHEFYL DYKDPVDKSG AEDTQLAYRS KRGAKWNAYA
     TFDQWTLGSQ YLYQGERFNG STRLPSYSLW NFTASYAVNQ NWDINAKLSN AFDKDYEMYV
     GYVTPGRQYF VSADYRF