ID   BTUB_VIBCH              Reviewed;         611 AA.
AC   Q9KVI9;
DT   15-MAR-2005, integrated into UniProtKB/Swiss-Prot.
DT   01-OCT-2000, sequence version 1.
DT   03-AUG-2022, entry version 115.
DE   RecName: Full=Vitamin B12 transporter BtuB {ECO:0000255|HAMAP-Rule:MF_01531};
DE   AltName: Full=Cobalamin receptor {ECO:0000255|HAMAP-Rule:MF_01531};
DE   AltName: Full=Outer membrane cobalamin translocator {ECO:0000255|HAMAP-Rule:MF_01531};
DE   Flags: Precursor;
GN   Name=btuB {ECO:0000255|HAMAP-Rule:MF_01531}; OrderedLocusNames=VC_0156;
OS   Vibrio cholerae serotype O1 (strain ATCC 39315 / El Tor Inaba N16961).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Vibrionales; Vibrionaceae;
OC   Vibrio.
OX   NCBI_TaxID=243277;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 39315 / El Tor Inaba N16961;
RX   PubMed=10952301; DOI=10.1038/35020000;
RA   Heidelberg J.F., Eisen J.A., Nelson W.C., Clayton R.A., Gwinn M.L.,
RA   Dodson R.J., Haft D.H., Hickey E.K., Peterson J.D., Umayam L.A., Gill S.R.,
RA   Nelson K.E., Read T.D., Tettelin H., Richardson D.L., Ermolaeva M.D.,
RA   Vamathevan J.J., Bass S., Qin H., Dragoi I., Sellers P., McDonald L.A.,
RA   Utterback T.R., Fleischmann R.D., Nierman W.C., White O., Salzberg S.L.,
RA   Smith H.O., Colwell R.R., Mekalanos J.J., Venter J.C., Fraser C.M.;
RT   "DNA sequence of both chromosomes of the cholera pathogen Vibrio
RT   cholerae.";
RL   Nature 406:477-483(2000).
CC   -!- FUNCTION: Involved in the active translocation of vitamin B12
CC       (cyanocobalamin) across the outer membrane to the periplasmic space. It
CC       derives its energy for transport by interacting with the trans-
CC       periplasmic membrane protein TonB. {ECO:0000255|HAMAP-Rule:MF_01531}.
CC   -!- SUBCELLULAR LOCATION: Cell outer membrane {ECO:0000255|HAMAP-
CC       Rule:MF_01531}; Multi-pass membrane protein {ECO:0000255|HAMAP-
CC       Rule:MF_01531}.
CC   -!- SIMILARITY: Belongs to the TonB-dependent receptor family. BtuB (TC
CC       1.B.14.3.1) subfamily. {ECO:0000255|HAMAP-Rule:MF_01531}.
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DR   EMBL; AE003852; AAF93332.1; -; Genomic_DNA.
DR   PIR; A82358; A82358.
DR   RefSeq; NP_229813.1; NC_002505.1.
DR   RefSeq; WP_001172310.1; NZ_LT906614.1.
DR   AlphaFoldDB; Q9KVI9; -.
DR   SMR; Q9KVI9; -.
DR   STRING; 243277.VC_0156; -.
DR   DNASU; 2612956; -.
DR   EnsemblBacteria; AAF93332; AAF93332; VC_0156.
DR   GeneID; 57741351; -.
DR   KEGG; vch:VC_0156; -.
DR   PATRIC; fig|243277.26.peg.143; -.
DR   eggNOG; COG4206; Bacteria.
DR   HOGENOM; CLU_008287_18_5_6; -.
DR   OMA; SYELQWR; -.
DR   BioCyc; VCHO:VC0156-MON; -.
DR   Proteomes; UP000000584; Chromosome 1.
DR   GO; GO:0031230; C:intrinsic component of cell outer membrane; IBA:GO_Central.
DR   GO; GO:0046930; C:pore complex; IEA:UniProtKB-KW.
DR   GO; GO:0015420; F:ABC-type vitamin B12 transporter activity; IEA:InterPro.
DR   GO; GO:0015288; F:porin activity; IEA:UniProtKB-KW.
DR   GO; GO:0015889; P:cobalamin transport; IBA:GO_Central.
DR   GO; GO:0006811; P:ion transport; IEA:UniProtKB-KW.
DR   Gene3D; 2.170.130.10; -; 1.
DR   Gene3D; 2.40.170.20; -; 1.
DR   HAMAP; MF_01531; BtuB; 1.
DR   InterPro; IPR010101; B12_transptr_BtuB.
DR   InterPro; IPR039426; BtuB-like.
DR   InterPro; IPR012910; Plug_dom.
DR   InterPro; IPR037066; Plug_dom_sf.
DR   InterPro; IPR000531; TonB-dep_rcpt_b-brl.
DR   InterPro; IPR010916; TonB_box_CS.
DR   InterPro; IPR036942; TonB_rcpt_b-brl_sf.
DR   InterPro; IPR010917; TonB_rcpt_CS.
DR   PANTHER; PTHR30069; PTHR30069; 1.
DR   Pfam; PF07715; Plug; 1.
DR   Pfam; PF00593; TonB_dep_Rec; 1.
DR   TIGRFAMs; TIGR01779; TonB-B12; 1.
DR   PROSITE; PS00430; TONB_DEPENDENT_REC_1; 1.
DR   PROSITE; PS01156; TONB_DEPENDENT_REC_2; 1.
PE   3: Inferred from homology;
KW   Cell outer membrane; Ion transport; Membrane; Porin; Reference proteome;
KW   Signal; TonB box; Transmembrane; Transmembrane beta strand; Transport.
FT   SIGNAL          1..22
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01531"
FT   CHAIN           23..611
FT                   /note="Vitamin B12 transporter BtuB"
FT                   /id="PRO_0000003491"
FT   MOTIF           29..36
FT                   /note="TonB box"
FT   MOTIF           594..611
FT                   /note="TonB C-terminal box"
SQ   SEQUENCE   611 AA;  68610 MW;  0C6D9A9A6A2063BE CRC64;
     MQKSALAIAL ASLLTPISYL HANEAQPQET VVVTANRFEQ KASSTLADVE IITRQDIEQT
     QAKTLPELLR RLTGVQITQN GGRGQLASLF VRGTSSDQVL VLVDGIRFAR AAKGAVDFNQ
     IPLTYVDRIE YVRGARASLY GSEAIGGVIN IITKARSQQQ GTTVSAGLGS LDYQELSIAS
     GVAIGEKGQM NVALGTESDK GYNVRPVPGV NDGDRHGFRS DNALLGYVHQ FDESWSLFAN
     ARAYENIYQY DNSYGTRDYK EAEKDDLSFT IGTQYQSERW VSELQLTTQK QKSWDYTQSK
     GKYSDTSDNL EQRNIQWINR YLVNDVWTFA GGVDWRDESY IDKTADKEFD RSNTAAFAVV
     AAEWQQWLLE ASLRFDDNQE YGSQTTHNIA LGYQFIPEFG VKASYGSAFK APNLYQQYDP
     SYGNVNLQPE DADSAELSFY GLFSGIKWSI TGYDYKINNL IDYNSTTKNY QNVIGESNIK
     GVEFTAEFAT GIVQHQLSVD LKDADDSKGK TLQRRAEHMY KWNALVAFEQ VDWSIGYQYV
     GKRPDLDYNT YPTQNITLDA YSLVDTSVSY YVTDSTTISA RIDNLLDKEY ETANGYPAAE
     RAYYLNIGYQ F