TPC6A_MOUSE
ID TPC6A_MOUSE Reviewed; 159 AA.
AC Q78XR0; Q3KQP0; Q8C2C8; Q9CQ27; Q9D8H6;
DT 19-JUL-2005, integrated into UniProtKB/Swiss-Prot.
DT 05-JUL-2004, sequence version 1.
DT 03-AUG-2022, entry version 118.
DE RecName: Full=Trafficking protein particle complex subunit 6A {ECO:0000250|UniProtKB:O75865};
DE Short=TRAPP complex subunit 6A;
GN Name=Trappc6a {ECO:0000303|PubMed:16697553, ECO:0000312|MGI:MGI:1914341};
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RC STRAIN=C57BL/6J, and NOD; TISSUE=Pancreas, Small intestine, and Thymus;
RX PubMed=16141072; DOI=10.1126/science.1112014;
RA Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N.,
RA Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K.,
RA Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.,
RA Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R.,
RA Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T.,
RA Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A.,
RA Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B.,
RA Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M.,
RA Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S.,
RA Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E.,
RA Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D.,
RA Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M.,
RA Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H.,
RA Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V.,
RA Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S.,
RA Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H.,
RA Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N.,
RA Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F.,
RA Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G.,
RA Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z.,
RA Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C.,
RA Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y.,
RA Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S.,
RA Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K.,
RA Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R.,
RA van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H.,
RA Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M.,
RA Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C.,
RA Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S.,
RA Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K.,
RA Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M.,
RA Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C.,
RA Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A.,
RA Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.;
RT "The transcriptional landscape of the mammalian genome.";
RL Science 309:1559-1563(2005).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
RC STRAIN=FVB/N; TISSUE=Mammary tumor, and Salivary gland;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [3]
RP FUNCTION, AND TISSUE SPECIFICITY.
RX PubMed=16697553; DOI=10.1016/j.ygeno.2006.04.002;
RA Gwynn B., Smith R.S., Rowe L.B., Taylor B.A., Peters L.L.;
RT "A mouse TRAPP-related protein is involved in pigmentation.";
RL Genomics 88:196-203(2006).
RN [4]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-33, AND IDENTIFICATION BY
RP MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Kidney;
RX PubMed=21183079; DOI=10.1016/j.cell.2010.12.001;
RA Huttlin E.L., Jedrychowski M.P., Elias J.E., Goswami T., Rad R.,
RA Beausoleil S.A., Villen J., Haas W., Sowa M.E., Gygi S.P.;
RT "A tissue-specific atlas of mouse protein phosphorylation and expression.";
RL Cell 143:1174-1189(2010).
CC -!- FUNCTION: May play a role in vesicular transport during the biogenesis
CC of melanosomes. {ECO:0000269|PubMed:16697553}.
CC -!- SUBUNIT: Part of the multisubunit transport protein particle (TRAPP)
CC complex. Heterodimer with TRAPPC3 (By similarity). The heterodimer
CC TRAPPC3-TRAPPC6A interacts with TRAPPC2L. Interacts with TRAPPC2L (By
CC similarity). {ECO:0000250|UniProtKB:O75865}.
CC -!- SUBCELLULAR LOCATION: Golgi apparatus, cis-Golgi network {ECO:0000250}.
CC Endoplasmic reticulum {ECO:0000250}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1;
CC IsoId=Q78XR0-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q78XR0-2; Sequence=VSP_019586;
CC -!- TISSUE SPECIFICITY: Ubiquitous, with lowest expression in skeletal
CC muscle and brain and highest in kidney, liver and testis, as well as in
CC cultured melanocytes. {ECO:0000269|PubMed:16697553}.
CC -!- SIMILARITY: Belongs to the TRAPP small subunits family. BET3 subfamily.
CC {ECO:0000305}.
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DR EMBL; AK007965; BAB25376.1; -; mRNA.
DR EMBL; AK008022; BAB25415.1; -; mRNA.
DR EMBL; AK008462; BAB25681.2; -; mRNA.
DR EMBL; AK088832; BAC40600.1; -; mRNA.
DR EMBL; BC037154; AAH37154.1; -; mRNA.
DR EMBL; BC106114; AAI06115.1; -; mRNA.
DR CCDS; CCDS20905.1; -. [Q78XR0-1]
DR CCDS; CCDS90174.1; -. [Q78XR0-2]
DR RefSeq; NP_001318110.1; NM_001331181.1. [Q78XR0-2]
DR RefSeq; NP_080236.2; NM_025960.4. [Q78XR0-1]
DR AlphaFoldDB; Q78XR0; -.
DR SMR; Q78XR0; -.
DR ComplexPortal; CPX-4766; TRAPP III complex.
DR STRING; 10090.ENSMUSP00000002112; -.
DR iPTMnet; Q78XR0; -.
DR PhosphoSitePlus; Q78XR0; -.
DR EPD; Q78XR0; -.
DR MaxQB; Q78XR0; -.
DR PaxDb; Q78XR0; -.
DR PRIDE; Q78XR0; -.
DR ProteomicsDB; 259500; -. [Q78XR0-1]
DR ProteomicsDB; 259501; -. [Q78XR0-2]
DR Antibodypedia; 49152; 138 antibodies from 26 providers.
DR Ensembl; ENSMUST00000002112; ENSMUSP00000002112; ENSMUSG00000002043. [Q78XR0-1]
DR Ensembl; ENSMUST00000108455; ENSMUSP00000104095; ENSMUSG00000002043. [Q78XR0-2]
DR GeneID; 67091; -.
DR KEGG; mmu:67091; -.
DR UCSC; uc009fmb.1; mouse. [Q78XR0-2]
DR UCSC; uc009fmc.1; mouse. [Q78XR0-1]
DR CTD; 79090; -.
DR MGI; MGI:1914341; Trappc6a.
DR VEuPathDB; HostDB:ENSMUSG00000002043; -.
DR eggNOG; KOG3316; Eukaryota.
DR GeneTree; ENSGT00390000012948; -.
DR HOGENOM; CLU_076409_3_1_1; -.
DR InParanoid; Q78XR0; -.
DR OMA; QVVIQKA; -.
DR OrthoDB; 1566836at2759; -.
DR PhylomeDB; Q78XR0; -.
DR TreeFam; TF313010; -.
DR Reactome; R-MMU-204005; COPII-mediated vesicle transport.
DR Reactome; R-MMU-8876198; RAB GEFs exchange GTP for GDP on RABs.
DR BioGRID-ORCS; 67091; 6 hits in 73 CRISPR screens.
DR ChiTaRS; Trappc6a; mouse.
DR PRO; PR:Q78XR0; -.
DR Proteomes; UP000000589; Chromosome 7.
DR RNAct; Q78XR0; protein.
DR Bgee; ENSMUSG00000002043; Expressed in lip and 202 other tissues.
DR ExpressionAtlas; Q78XR0; baseline and differential.
DR Genevisible; Q78XR0; MM.
DR GO; GO:0005801; C:cis-Golgi network; IBA:GO_Central.
DR GO; GO:0005737; C:cytoplasm; IC:ComplexPortal.
DR GO; GO:0005783; C:endoplasmic reticulum; IEA:UniProtKB-SubCell.
DR GO; GO:0005802; C:trans-Golgi network; IBA:GO_Central.
DR GO; GO:0030008; C:TRAPP complex; IBA:GO_Central.
DR GO; GO:1990072; C:TRAPPIII protein complex; IC:ComplexPortal.
DR GO; GO:0048208; P:COPII vesicle coating; IC:ComplexPortal.
DR GO; GO:0006888; P:endoplasmic reticulum to Golgi vesicle-mediated transport; IBA:GO_Central.
DR GO; GO:1903232; P:melanosome assembly; IBA:GO_Central.
DR GO; GO:0043087; P:regulation of GTPase activity; IEA:InterPro.
DR GO; GO:0099022; P:vesicle tethering; IC:ComplexPortal.
DR CDD; cd14944; TRAPPC6A_Trs33; 1.
DR InterPro; IPR024096; NO_sig/Golgi_transp_ligand-bd.
DR InterPro; IPR007194; TRAPP_component.
DR InterPro; IPR037992; TRAPPC6/Trs33.
DR PANTHER; PTHR12817; PTHR12817; 1.
DR Pfam; PF04051; TRAPP; 1.
DR SUPFAM; SSF111126; SSF111126; 1.
PE 1: Evidence at protein level;
KW Alternative splicing; Endoplasmic reticulum; ER-Golgi transport;
KW Golgi apparatus; Phosphoprotein; Reference proteome; Transport.
FT CHAIN 1..159
FT /note="Trafficking protein particle complex subunit 6A"
FT /id="PRO_0000211586"
FT MOD_RES 33
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:21183079"
FT VAR_SEQ 151..159
FT /note="KFQVVIQKS -> RLEAGAHRLWGGHMCFRQGNERAGRVPGGGTIP (in
FT isoform 2)"
FT /evidence="ECO:0000303|PubMed:15489334"
FT /id="VSP_019586"
FT CONFLICT 4
FT /note="A -> V (in Ref. 1; BAB25415)"
FT /evidence="ECO:0000305"
FT CONFLICT 54
FT /note="L -> R (in Ref. 1; BAC40600)"
FT /evidence="ECO:0000305"
FT CONFLICT 70
FT /note="C -> S (in Ref. 1; BAB25415)"
FT /evidence="ECO:0000305"
FT CONFLICT 114
FT /note="E -> K (in Ref. 1; BAB25681)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 159 AA; 17430 MW; D3A80F6C88ABD152 CRC64;
MADAVLFEFL HTEMVAELWA PDPDPGSGGK RRSLSVLEGL GFRVGQALGE RLPLETPAFR
EELDALKFLC RDLWAAMFQK HMDGLRTNHQ GTYVLQDNSF PLLVTMGSGP QYLEEAPKFL
AFTCGLLCGA LHTLGFQSLV TASVASLPAC KFQVVIQKS
