TPCD_ASPFU
ID TPCD_ASPFU Reviewed; 437 AA.
AC Q4WQZ4;
DT 07-SEP-2016, integrated into UniProtKB/Swiss-Prot.
DT 05-JUL-2005, sequence version 1.
DT 03-AUG-2022, entry version 66.
DE RecName: Full=Trypacidin cluster transcriptional coactivator tpcD {ECO:0000303|PubMed:26278536};
DE AltName: Full=Trypacidin synthesis protein D {ECO:0000303|PubMed:26242966};
GN Name=tpcD {ECO:0000303|PubMed:26242966};
GN Synonyms=tynD {ECO:0000303|PubMed:26278536}; ORFNames=AFUA_4G14550;
OS Neosartorya fumigata (strain ATCC MYA-4609 / Af293 / CBS 101355 / FGSC
OS A1100) (Aspergillus fumigatus).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Pezizomycotina; Eurotiomycetes;
OC Eurotiomycetidae; Eurotiales; Aspergillaceae; Aspergillus;
OC Aspergillus subgen. Fumigati.
OX NCBI_TaxID=330879;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC MYA-4609 / Af293 / CBS 101355 / FGSC A1100;
RX PubMed=16372009; DOI=10.1038/nature04332;
RA Nierman W.C., Pain A., Anderson M.J., Wortman J.R., Kim H.S., Arroyo J.,
RA Berriman M., Abe K., Archer D.B., Bermejo C., Bennett J.W., Bowyer P.,
RA Chen D., Collins M., Coulsen R., Davies R., Dyer P.S., Farman M.L.,
RA Fedorova N., Fedorova N.D., Feldblyum T.V., Fischer R., Fosker N.,
RA Fraser A., Garcia J.L., Garcia M.J., Goble A., Goldman G.H., Gomi K.,
RA Griffith-Jones S., Gwilliam R., Haas B.J., Haas H., Harris D.E.,
RA Horiuchi H., Huang J., Humphray S., Jimenez J., Keller N., Khouri H.,
RA Kitamoto K., Kobayashi T., Konzack S., Kulkarni R., Kumagai T., Lafton A.,
RA Latge J.-P., Li W., Lord A., Lu C., Majoros W.H., May G.S., Miller B.L.,
RA Mohamoud Y., Molina M., Monod M., Mouyna I., Mulligan S., Murphy L.D.,
RA O'Neil S., Paulsen I., Penalva M.A., Pertea M., Price C., Pritchard B.L.,
RA Quail M.A., Rabbinowitsch E., Rawlins N., Rajandream M.A., Reichard U.,
RA Renauld H., Robson G.D., Rodriguez de Cordoba S., Rodriguez-Pena J.M.,
RA Ronning C.M., Rutter S., Salzberg S.L., Sanchez M., Sanchez-Ferrero J.C.,
RA Saunders D., Seeger K., Squares R., Squares S., Takeuchi M., Tekaia F.,
RA Turner G., Vazquez de Aldana C.R., Weidman J., White O., Woodward J.R.,
RA Yu J.-H., Fraser C.M., Galagan J.E., Asai K., Machida M., Hall N.,
RA Barrell B.G., Denning D.W.;
RT "Genomic sequence of the pathogenic and allergenic filamentous fungus
RT Aspergillus fumigatus.";
RL Nature 438:1151-1156(2005).
RN [2]
RP FUNCTION, AND TISSUE SPECIFICITY.
RX PubMed=22319557; DOI=10.1371/journal.pone.0029906;
RA Gauthier T., Wang X., Sifuentes Dos Santos J., Fysikopoulos A., Tadrist S.,
RA Canlet C., Artigot M.P., Loiseau N., Oswald I.P., Puel O.;
RT "Trypacidin, a spore-borne toxin from Aspergillus fumigatus, is cytotoxic
RT to lung cells.";
RL PLoS ONE 7:E29906-E29906(2012).
RN [3]
RP FUNCTION.
RX PubMed=26278536; DOI=10.1007/s00253-015-6898-1;
RA Mattern D.J., Schoeler H., Weber J., Novohradska S., Kraibooj K.,
RA Dahse H.M., Hillmann F., Valiante V., Figge M.T., Brakhage A.A.;
RT "Identification of the antiphagocytic trypacidin gene cluster in the human-
RT pathogenic fungus Aspergillus fumigatus.";
RL Appl. Microbiol. Biotechnol. 99:10151-10161(2015).
RN [4]
RP FUNCTION, DISRUPTION PHENOTYPE, AND INDUCTION.
RX PubMed=26242966; DOI=10.1111/1462-2920.13007;
RA Throckmorton K., Lim F.Y., Kontoyiannis D.P., Zheng W., Keller N.P.;
RT "Redundant synthesis of a conidial polyketide by two distinct secondary
RT metabolite clusters in Aspergillus fumigatus.";
RL Environ. Microbiol. 18:246-259(2016).
CC -!- FUNCTION: Transcriptional coactivator; part of the gene cluster that
CC mediates the biosynthesis of trypacidin, a mycotoxin with antiprotozoal
CC activity and that plays a role in the infection process
CC (PubMed:26278536, PubMed:26242966). With tpcE, coregulates the
CC production of trypacidin (PubMed:26278536, PubMed:26242966).
CC {ECO:0000269|PubMed:26242966, ECO:0000269|PubMed:26278536}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000305}.
CC -!- TISSUE SPECIFICITY: Specifically expressed in conidia
CC (PubMed:22319557). {ECO:0000305|PubMed:22319557}.
CC -!- INDUCTION: Expression is positively regulated by the transcription
CC factors brlA and laeA (PubMed:26242966). {ECO:0000269|PubMed:26242966}.
CC -!- DISRUPTION PHENOTYPE: Resulted in loss of trypacidin production
CC (PubMed:26278536, PubMed:26242966). {ECO:0000269|PubMed:26242966,
CC ECO:0000269|PubMed:26278536}.
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DR EMBL; AAHF01000005; EAL89340.1; -; Genomic_DNA.
DR RefSeq; XP_751378.1; XM_746285.1.
DR AlphaFoldDB; Q4WQZ4; -.
DR SMR; Q4WQZ4; -.
DR STRING; 746128.CADAFUBP00006996; -.
DR EnsemblFungi; EAL89340; EAL89340; AFUA_4G14550.
DR GeneID; 3509596; -.
DR KEGG; afm:AFUA_4G14550; -.
DR VEuPathDB; FungiDB:Afu4g14550; -.
DR eggNOG; ENOG502SM28; Eukaryota.
DR HOGENOM; CLU_005533_11_0_1; -.
DR InParanoid; Q4WQZ4; -.
DR OMA; LLACIQW; -.
DR OrthoDB; 817726at2759; -.
DR Proteomes; UP000002530; Chromosome 4.
DR GO; GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR GO; GO:0044550; P:secondary metabolite biosynthetic process; IMP:AspGD.
DR Gene3D; 1.10.10.10; -; 1.
DR Gene3D; 3.40.50.150; -; 1.
DR InterPro; IPR029063; SAM-dependent_MTases_sf.
DR InterPro; IPR036388; WH-like_DNA-bd_sf.
DR InterPro; IPR036390; WH_DNA-bd_sf.
DR SUPFAM; SSF46785; SSF46785; 1.
PE 2: Evidence at transcript level;
KW DNA-binding; Nucleus; Reference proteome; Transcription;
KW Transcription regulation.
FT CHAIN 1..437
FT /note="Trypacidin cluster transcriptional coactivator tpcD"
FT /id="PRO_0000437061"
FT DOMAIN 75..144
FT /note="HTH iclR-type"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00393"
FT DNA_BIND 105..124
FT /note="H-T-H motif"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00393"
SQ SEQUENCE 437 AA; 47865 MW; 1E2CF1B5FC18F174 CRC64;
MDSITQLEAR ANEVAAAARR LAEYCRNARV DGACLPAVVP SEAPRAIREI QRLLLSNVDH
LQILLTEPAD LVQRLAVQSQ LLSCLQWLGE FQVLACLPLT DTVSIADLAR LSGVPEAQLA
RIIRFTITIG FLQEPQRGHV AHSPLSSLFV SRPSLRDAVM FLADSAAPTA LQMASATGRF
GDTVASETDT AYNIAFDHRD PFYFACEQRP KLQRRWSAYL QHTGGDARDL TQQVLSRVDW
FNLNNACVVE VLGPQSQSVT MMLDQLHPML HFIVQEARIP NGSTHAALHQ DVRSVQIREL
GGPQRVRDAA IYILNLGPLP HAILSTSVLT ELRAHFSVLA ANSCAMLILT AGLLLPKPGA
VDARVETSVR LHDLSLLQLA NDRLMEEDEL VEMVHGVKDS VGRLAVVNRL HLPCTTTVAL
GVRYQAFGHG DSSAKSL
