TPCE_ASPFU
ID TPCE_ASPFU Reviewed; 396 AA.
AC Q4WQZ3;
DT 07-SEP-2016, integrated into UniProtKB/Swiss-Prot.
DT 17-APR-2007, sequence version 2.
DT 25-MAY-2022, entry version 91.
DE RecName: Full=Trypacidin cluster transcription factor {ECO:0000303|PubMed:26242966};
DE AltName: Full=Trypacidin synthesis protein E {ECO:0000303|PubMed:26242966};
GN Name=tpcE {ECO:0000303|PubMed:26242966};
GN Synonyms=tynE {ECO:0000303|PubMed:26278536}; ORFNames=AFUA_4G14540;
OS Neosartorya fumigata (strain ATCC MYA-4609 / Af293 / CBS 101355 / FGSC
OS A1100) (Aspergillus fumigatus).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Pezizomycotina; Eurotiomycetes;
OC Eurotiomycetidae; Eurotiales; Aspergillaceae; Aspergillus;
OC Aspergillus subgen. Fumigati.
OX NCBI_TaxID=330879;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC MYA-4609 / Af293 / CBS 101355 / FGSC A1100;
RX PubMed=16372009; DOI=10.1038/nature04332;
RA Nierman W.C., Pain A., Anderson M.J., Wortman J.R., Kim H.S., Arroyo J.,
RA Berriman M., Abe K., Archer D.B., Bermejo C., Bennett J.W., Bowyer P.,
RA Chen D., Collins M., Coulsen R., Davies R., Dyer P.S., Farman M.L.,
RA Fedorova N., Fedorova N.D., Feldblyum T.V., Fischer R., Fosker N.,
RA Fraser A., Garcia J.L., Garcia M.J., Goble A., Goldman G.H., Gomi K.,
RA Griffith-Jones S., Gwilliam R., Haas B.J., Haas H., Harris D.E.,
RA Horiuchi H., Huang J., Humphray S., Jimenez J., Keller N., Khouri H.,
RA Kitamoto K., Kobayashi T., Konzack S., Kulkarni R., Kumagai T., Lafton A.,
RA Latge J.-P., Li W., Lord A., Lu C., Majoros W.H., May G.S., Miller B.L.,
RA Mohamoud Y., Molina M., Monod M., Mouyna I., Mulligan S., Murphy L.D.,
RA O'Neil S., Paulsen I., Penalva M.A., Pertea M., Price C., Pritchard B.L.,
RA Quail M.A., Rabbinowitsch E., Rawlins N., Rajandream M.A., Reichard U.,
RA Renauld H., Robson G.D., Rodriguez de Cordoba S., Rodriguez-Pena J.M.,
RA Ronning C.M., Rutter S., Salzberg S.L., Sanchez M., Sanchez-Ferrero J.C.,
RA Saunders D., Seeger K., Squares R., Squares S., Takeuchi M., Tekaia F.,
RA Turner G., Vazquez de Aldana C.R., Weidman J., White O., Woodward J.R.,
RA Yu J.-H., Fraser C.M., Galagan J.E., Asai K., Machida M., Hall N.,
RA Barrell B.G., Denning D.W.;
RT "Genomic sequence of the pathogenic and allergenic filamentous fungus
RT Aspergillus fumigatus.";
RL Nature 438:1151-1156(2005).
RN [2]
RP FUNCTION, AND TISSUE SPECIFICITY.
RX PubMed=22319557; DOI=10.1371/journal.pone.0029906;
RA Gauthier T., Wang X., Sifuentes Dos Santos J., Fysikopoulos A., Tadrist S.,
RA Canlet C., Artigot M.P., Loiseau N., Oswald I.P., Puel O.;
RT "Trypacidin, a spore-borne toxin from Aspergillus fumigatus, is cytotoxic
RT to lung cells.";
RL PLoS ONE 7:E29906-E29906(2012).
RN [3]
RP FUNCTION.
RX PubMed=26278536; DOI=10.1007/s00253-015-6898-1;
RA Mattern D.J., Schoeler H., Weber J., Novohradska S., Kraibooj K.,
RA Dahse H.M., Hillmann F., Valiante V., Figge M.T., Brakhage A.A.;
RT "Identification of the antiphagocytic trypacidin gene cluster in the human-
RT pathogenic fungus Aspergillus fumigatus.";
RL Appl. Microbiol. Biotechnol. 99:10151-10161(2015).
RN [4]
RP FUNCTION, DISRUPTION PHENOTYPE, AND INDUCTION.
RX PubMed=26242966; DOI=10.1111/1462-2920.13007;
RA Throckmorton K., Lim F.Y., Kontoyiannis D.P., Zheng W., Keller N.P.;
RT "Redundant synthesis of a conidial polyketide by two distinct secondary
RT metabolite clusters in Aspergillus fumigatus.";
RL Environ. Microbiol. 18:246-259(2016).
CC -!- FUNCTION: Transcription factor that regulates the expression of the
CC gene clusters that mediate the biosynthesis of trypacidin, a metabolite
CC with antiprotozoal activity and a possible role in the infection
CC process (PubMed:26278536, PubMed:26242966). Trypacidin is toxic for
CC human pulmonary and bronchial epithelial cells by initiating the
CC intracellular formation of nitric oxide (NO) and hydrogen peroxide
CC (H(2)O(2)), thus triggering host necrotic cell death (PubMed:22319557).
CC {ECO:0000269|PubMed:22319557, ECO:0000269|PubMed:26242966,
CC ECO:0000269|PubMed:26278536}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000255|PROSITE-ProRule:PRU00227}.
CC -!- TISSUE SPECIFICITY: Specifically expressed in conidia
CC (PubMed:22319557). {ECO:0000305|PubMed:22319557}.
CC -!- INDUCTION: Expression is positively regulated by the transcription
CC factors brlA and laeA (PubMed:26242966). {ECO:0000269|PubMed:26242966}.
CC -!- DISRUPTION PHENOTYPE: Resulted in loss of trypacidin and questin
CC production (PubMed:26242966). {ECO:0000269|PubMed:26242966}.
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DR EMBL; AAHF01000005; EAL89341.2; -; Genomic_DNA.
DR RefSeq; XP_751379.2; XM_746286.2.
DR AlphaFoldDB; Q4WQZ3; -.
DR SMR; Q4WQZ3; -.
DR STRING; 746128.CADAFUBP00006995; -.
DR EnsemblFungi; EAL89341; EAL89341; AFUA_4G14540.
DR GeneID; 3509597; -.
DR KEGG; afm:AFUA_4G14540; -.
DR VEuPathDB; FungiDB:Afu4g14540; -.
DR eggNOG; ENOG502SUW5; Eukaryota.
DR HOGENOM; CLU_031656_1_0_1; -.
DR InParanoid; Q4WQZ3; -.
DR OMA; PEPFDST; -.
DR OrthoDB; 1396569at2759; -.
DR Proteomes; UP000002530; Chromosome 4.
DR GO; GO:0005634; C:nucleus; IBA:GO_Central.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR GO; GO:0000981; F:DNA-binding transcription factor activity, RNA polymerase II-specific; IBA:GO_Central.
DR GO; GO:0008270; F:zinc ion binding; IEA:InterPro.
DR GO; GO:0045122; P:aflatoxin biosynthetic process; IEA:InterPro.
DR GO; GO:0045944; P:positive regulation of transcription by RNA polymerase II; IBA:GO_Central.
DR GO; GO:0044550; P:secondary metabolite biosynthetic process; IMP:AspGD.
DR CDD; cd00067; GAL4; 1.
DR Gene3D; 4.10.240.10; -; 1.
DR InterPro; IPR013700; AflR.
DR InterPro; IPR001138; Zn2-C6_fun-type_DNA-bd.
DR InterPro; IPR036864; Zn2-C6_fun-type_DNA-bd_sf.
DR Pfam; PF08493; AflR; 1.
DR Pfam; PF00172; Zn_clus; 1.
DR SMART; SM00066; GAL4; 1.
DR SUPFAM; SSF57701; SSF57701; 1.
DR PROSITE; PS50048; ZN2_CY6_FUNGAL_2; 1.
PE 2: Evidence at transcript level;
KW DNA-binding; Metal-binding; Nucleus; Reference proteome; Transcription;
KW Transcription regulation.
FT CHAIN 1..396
FT /note="Trypacidin cluster transcription factor"
FT /id="PRO_0000437059"
FT DNA_BIND 20..47
FT /note="Zn(2)-C6 fungal-type"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00227"
FT REGION 54..120
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 190..218
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 346..365
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 54..69
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 396 AA; 43111 MW; 991F8E3D0B9094F1 CRC64;
MRSPASSRRA SALHRLCDSC RACGLSKVRC SKEKPTCSRC RRRGTVCEYV VTKRPGRKPD
SRSEVEPEPG HLSHPLPSPE SSTAISQGNL PDPDAFDPLF ALPEPFDSTP ELHSGPLSTV
DSSLSSALTT WCPDFDDFFS FPVSTEDTLS GDRGAVYDGT IKHPPNLRRH SESPPAVPDD
TIYLFGKPVD PAPPDQSLPA TTTIGRRASP PSGRESEAGS RRLSCRCLIR ALDLLKHFEV
IRREAEIESS LPSIDAVVEV NKQTTEAITG MLQCPCSQKD GYLLVLLALI VCKILDRYAA
AAIPGTEQGH DPTRRLDEGP MAGQRVLGEL HCIQRVMNQL GPRLRMHGAQ GASPGQAFHG
TPAPLSVGLG DQLEPELHQR LSRLSSEIIR LLREAQ
