TPCH_ASPFU
ID TPCH_ASPFU Reviewed; 282 AA.
AC Q4WQZ0;
DT 07-SEP-2016, integrated into UniProtKB/Swiss-Prot.
DT 17-APR-2007, sequence version 2.
DT 03-AUG-2022, entry version 72.
DE RecName: Full=Methyltransferase tpcH {ECO:0000303|PubMed:26242966};
DE EC=2.1.1.- {ECO:0000305|PubMed:26242966};
DE AltName: Full=Trypacidin synthesis protein H {ECO:0000303|PubMed:26242966};
GN Name=tpcH {ECO:0000303|PubMed:26242966};
GN Synonyms=tynH {ECO:0000303|PubMed:26278536}; ORFNames=AFUA_4G14510;
OS Neosartorya fumigata (strain ATCC MYA-4609 / Af293 / CBS 101355 / FGSC
OS A1100) (Aspergillus fumigatus).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Pezizomycotina; Eurotiomycetes;
OC Eurotiomycetidae; Eurotiales; Aspergillaceae; Aspergillus;
OC Aspergillus subgen. Fumigati.
OX NCBI_TaxID=330879;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC MYA-4609 / Af293 / CBS 101355 / FGSC A1100;
RX PubMed=16372009; DOI=10.1038/nature04332;
RA Nierman W.C., Pain A., Anderson M.J., Wortman J.R., Kim H.S., Arroyo J.,
RA Berriman M., Abe K., Archer D.B., Bermejo C., Bennett J.W., Bowyer P.,
RA Chen D., Collins M., Coulsen R., Davies R., Dyer P.S., Farman M.L.,
RA Fedorova N., Fedorova N.D., Feldblyum T.V., Fischer R., Fosker N.,
RA Fraser A., Garcia J.L., Garcia M.J., Goble A., Goldman G.H., Gomi K.,
RA Griffith-Jones S., Gwilliam R., Haas B.J., Haas H., Harris D.E.,
RA Horiuchi H., Huang J., Humphray S., Jimenez J., Keller N., Khouri H.,
RA Kitamoto K., Kobayashi T., Konzack S., Kulkarni R., Kumagai T., Lafton A.,
RA Latge J.-P., Li W., Lord A., Lu C., Majoros W.H., May G.S., Miller B.L.,
RA Mohamoud Y., Molina M., Monod M., Mouyna I., Mulligan S., Murphy L.D.,
RA O'Neil S., Paulsen I., Penalva M.A., Pertea M., Price C., Pritchard B.L.,
RA Quail M.A., Rabbinowitsch E., Rawlins N., Rajandream M.A., Reichard U.,
RA Renauld H., Robson G.D., Rodriguez de Cordoba S., Rodriguez-Pena J.M.,
RA Ronning C.M., Rutter S., Salzberg S.L., Sanchez M., Sanchez-Ferrero J.C.,
RA Saunders D., Seeger K., Squares R., Squares S., Takeuchi M., Tekaia F.,
RA Turner G., Vazquez de Aldana C.R., Weidman J., White O., Woodward J.R.,
RA Yu J.-H., Fraser C.M., Galagan J.E., Asai K., Machida M., Hall N.,
RA Barrell B.G., Denning D.W.;
RT "Genomic sequence of the pathogenic and allergenic filamentous fungus
RT Aspergillus fumigatus.";
RL Nature 438:1151-1156(2005).
RN [2]
RP FUNCTION, AND TISSUE SPECIFICITY.
RX PubMed=22319557; DOI=10.1371/journal.pone.0029906;
RA Gauthier T., Wang X., Sifuentes Dos Santos J., Fysikopoulos A., Tadrist S.,
RA Canlet C., Artigot M.P., Loiseau N., Oswald I.P., Puel O.;
RT "Trypacidin, a spore-borne toxin from Aspergillus fumigatus, is cytotoxic
RT to lung cells.";
RL PLoS ONE 7:E29906-E29906(2012).
RN [3]
RP FUNCTION.
RX PubMed=26278536; DOI=10.1007/s00253-015-6898-1;
RA Mattern D.J., Schoeler H., Weber J., Novohradska S., Kraibooj K.,
RA Dahse H.M., Hillmann F., Valiante V., Figge M.T., Brakhage A.A.;
RT "Identification of the antiphagocytic trypacidin gene cluster in the human-
RT pathogenic fungus Aspergillus fumigatus.";
RL Appl. Microbiol. Biotechnol. 99:10151-10161(2015).
RN [4]
RP FUNCTION, AND INDUCTION.
RX PubMed=26242966; DOI=10.1111/1462-2920.13007;
RA Throckmorton K., Lim F.Y., Kontoyiannis D.P., Zheng W., Keller N.P.;
RT "Redundant synthesis of a conidial polyketide by two distinct secondary
RT metabolite clusters in Aspergillus fumigatus.";
RL Environ. Microbiol. 18:246-259(2016).
CC -!- FUNCTION: Methyltransferase; part of the gene cluster that mediates the
CC biosynthesis of trypacidin, a mycotoxin with antiprotozoal activity and
CC that plays a role in the infection process (PubMed:26278536,
CC PubMed:26242966). The pathway begins with the synthesis of atrochrysone
CC thioester by the polyketide synthase (PKS) tpcC (PubMed:26242966). The
CC atrochrysone carboxyl ACP thioesterase tpcB then breaks the thioester
CC bond and releases the atrochrysone carboxylic acid from tpcC
CC (PubMed:26242966). The decarboxylase tpcK converts atrochrysone
CC carboxylic acid to atrochrysone which is further reduced into emodin
CC anthrone (PubMed:26242966). The next step is performed by the emodin
CC anthrone oxygenase tpcL that catalyzes the oxidation of emodinanthrone
CC to emodin (PubMed:26242966). Emodin O-methyltransferase encoded by tpcA
CC catalyzes methylation of the 8-hydroxy group of emodin to form questin
CC (PubMed:26242966). Ring cleavage of questin by questin oxidase tpcI
CC leads to desmethylsulochrin via several intermediates including questin
CC epoxide (By similarity). Another methylation step catalyzed by tpcM
CC leads to the formation of sulochrin which is further converted to
CC monomethylsulfochrin by tpcH. Finally, the tpcJ catalyzes the
CC conversion of monomethylsulfochrin to trypacidin (PubMed:26242966).
CC Trypacidin is toxic for human pulmonary and bronchial epithelial cells
CC by initiating the intracellular formation of nitric oxide (NO) and
CC hydrogen peroxide (H(2)O(2)), thus triggering host necrotic cell death
CC (PubMed:22319557). The trypacidin pathway is also able to produce
CC endocrocin via a distinct route from the endocrocin Enc pathway
CC (PubMed:26242966). {ECO:0000250|UniProtKB:Q0CCX8,
CC ECO:0000269|PubMed:22319557, ECO:0000269|PubMed:26242966,
CC ECO:0000269|PubMed:26278536}.
CC -!- PATHWAY: Secondary metabolite biosynthesis.
CC {ECO:0000305|PubMed:26242966}.
CC -!- TISSUE SPECIFICITY: Specifically expressed in conidia
CC (PubMed:22319557). {ECO:0000305|PubMed:22319557}.
CC -!- INDUCTION: Expression is positively regulated by the transcription
CC factors brlA and laeA (PubMed:26242966). {ECO:0000269|PubMed:26242966}.
CC -!- SIMILARITY: Belongs to the class I-like SAM-binding methyltransferase
CC superfamily. {ECO:0000305}.
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DR EMBL; AAHF01000005; EAL89344.2; -; Genomic_DNA.
DR RefSeq; XP_751382.2; XM_746289.2.
DR AlphaFoldDB; Q4WQZ0; -.
DR SMR; Q4WQZ0; -.
DR STRING; 746128.CADAFUBP00006993; -.
DR EnsemblFungi; EAL89344; EAL89344; AFUA_4G14510.
DR GeneID; 3509606; -.
DR KEGG; afm:AFUA_4G14510; -.
DR VEuPathDB; FungiDB:Afu4g14510; -.
DR eggNOG; ENOG502S4V1; Eukaryota.
DR HOGENOM; CLU_065416_0_0_1; -.
DR InParanoid; Q4WQZ0; -.
DR OMA; HEMLPDW; -.
DR OrthoDB; 785883at2759; -.
DR Proteomes; UP000002530; Chromosome 4.
DR GO; GO:0008168; F:methyltransferase activity; IBA:GO_Central.
DR GO; GO:0032259; P:methylation; IEA:UniProtKB-KW.
DR GO; GO:0044550; P:secondary metabolite biosynthetic process; IGC:AspGD.
DR Gene3D; 3.40.50.150; -; 1.
DR InterPro; IPR041698; Methyltransf_25.
DR InterPro; IPR029063; SAM-dependent_MTases_sf.
DR Pfam; PF13649; Methyltransf_25; 1.
DR SUPFAM; SSF53335; SSF53335; 1.
PE 2: Evidence at transcript level;
KW Methyltransferase; Reference proteome; S-adenosyl-L-methionine;
KW Transferase.
FT CHAIN 1..282
FT /note="Methyltransferase tpcH"
FT /id="PRO_0000437104"
SQ SEQUENCE 282 AA; 31896 MW; 2C0CACAFDAACB345 CRC64;
MLEKVFHEKS FADQYTYGAK ISELYAETLI TESGIAKSHQ RPLIILDNAC GTGSISSTLQ
RTLDERNKRS LKLTCGDLSE GMVDYTKQRM QAEGWNNAEA KIVNAQDTGL PSDHYTHVYT
AFAFNMFPDY KAALRECLRI LQPGGTLATS TWKTANWCTI MKPVIATMPG QLSYPTMDEI
NTMLNKGWDR ESDVRAEFEQ AGFDHVNITT VEKQCLLPVQ EFGEACKILL PYILSKFWTQ
EQRDQYEADV PSYLMRYLER EYGKDGLAPM KGVAIIASGR KP
