ID   TPES_PSEPU              Reviewed;         272 AA.
AC   P07383;
DT   01-APR-1988, integrated into UniProtKB/Swiss-Prot.
DT   01-APR-1988, sequence version 1.
DT   25-MAY-2022, entry version 78.
DE   RecName: Full=Tropinesterase;
DE            EC=3.1.1.10;
DE   AltName: Full=Atropine acylhydrolase;
DE   AltName: Full=Atropinesterase;
OS   Pseudomonas putida (Arthrobacter siderocapsulatus).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Pseudomonadales;
OC   Pseudomonadaceae; Pseudomonas.
OX   NCBI_TaxID=303;
RN   [1]
RP   PROTEIN SEQUENCE.
RC   STRAIN=L / Biotype A;
RA   Hessing J.G.M.;
RL   Thesis (1983), University of Leiden, Netherlands.
CC   -!- FUNCTION: Alkaloid degradation.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=atropine + H2O = H(+) + tropate + tropine;
CC         Xref=Rhea:RHEA:23304, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378,
CC         ChEBI:CHEBI:17000, ChEBI:CHEBI:57554, ChEBI:CHEBI:57858; EC=3.1.1.10;
CC   -!- PATHWAY: Alkaloid degradation; atropine degradation.
CC   -!- SUBUNIT: Homodimer.
CC   -!- MISCELLANEOUS: The strain used in this study was isolated from the soil
CC       between the roots of belladonna (Atropa belladonna).
CC   -!- SIMILARITY: Belongs to the DmpD/TodF/XylF esterase family.
CC       {ECO:0000305}.
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   PIR; A26563; ESPSTP.
DR   AlphaFoldDB; P07383; -.
DR   SMR; P07383; -.
DR   ESTHER; psepu-tpes; AlphaBeta_hydrolase.
DR   UniPathway; UPA00303; -.
DR   GO; GO:0050357; F:tropinesterase activity; IEA:UniProtKB-EC.
DR   GO; GO:0009820; P:alkaloid metabolic process; IEA:UniProtKB-KW.
DR   Gene3D; 3.40.50.1820; -; 1.
DR   InterPro; IPR029058; AB_hydrolase.
DR   InterPro; IPR000073; AB_hydrolase_1.
DR   Pfam; PF00561; Abhydrolase_1; 1.
DR   PRINTS; PR00111; ABHYDROLASE.
DR   SUPFAM; SSF53474; SSF53474; 1.
PE   1: Evidence at protein level;
KW   Alkaloid metabolism; Direct protein sequencing; Hydrolase; Serine esterase.
FT   CHAIN           1..272
FT                   /note="Tropinesterase"
FT                   /id="PRO_0000207056"
FT   DOMAIN          38..260
FT                   /note="AB hydrolase-1"
FT                   /evidence="ECO:0000255"
FT   ACT_SITE        110
FT   ACT_SITE        253
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   272 AA;  30264 MW;  E0C9E3AB0112D236 CRC64;
     EIIPVPDQAA WNASKKSIQI NDAIKMRYVE WGNPSGDPVL LLHGYTDTSR AFSSLAPFLS
     KDKRYLALDL RGHGGTSIPK CCYYVSDFAE DVSDFIDKMG LHNTTVIGHS MGSMTAGVLA
     SIHPDKVSRL VLISTALKTG PVLEWVYDTV LQKDFPLDDP SEFAKEWVAA PGKHDNGMAK
     NLKTEELAVP KHVWLSAARG FSIINWTAAS KYLTAKTLIL WGNQNQPMTE SMQNDIRAAL
     PKAKFIQYNG FGHSMFWEDP EMVAKDLNEF LK