TPGS2_HUMAN
ID TPGS2_HUMAN Reviewed; 300 AA.
AC Q68CL5; B4DIX2; K7EIJ9; Q4KN59; Q8WTU3; Q96BT9; Q9Y435;
DT 19-JUL-2005, integrated into UniProtKB/Swiss-Prot.
DT 05-SEP-2006, sequence version 2.
DT 03-AUG-2022, entry version 134.
DE RecName: Full=Tubulin polyglutamylase complex subunit 2;
DE Short=PGs2;
GN Name=TPGS2; Synonyms=C18orf10; ORFNames=HMFN0601, L17;
OS Homo sapiens (Human).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae;
OC Homo.
OX NCBI_TaxID=9606;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 4).
RC TISSUE=Hippocampus;
RX PubMed=14702039; DOI=10.1038/ng1285;
RA Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R.,
RA Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H.,
RA Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.,
RA Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K.,
RA Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H.,
RA Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M.,
RA Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K.,
RA Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T.,
RA Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M.,
RA Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S.,
RA Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H.,
RA Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K.,
RA Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N.,
RA Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S.,
RA Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O.,
RA Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H.,
RA Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B.,
RA Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y.,
RA Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K.,
RA Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T.,
RA Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T.,
RA Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y.,
RA Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H.,
RA Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y.,
RA Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H.,
RA Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O.,
RA Isogai T., Sugano S.;
RT "Complete sequencing and characterization of 21,243 full-length human
RT cDNAs.";
RL Nat. Genet. 36:40-45(2004).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RC TISSUE=Liver;
RX PubMed=15221005; DOI=10.1038/sj.onc.1207782;
RA Yamada S., Ohira M., Horie H., Ando K., Takayasu H., Suzuki Y., Sugano S.,
RA Hirata T., Goto T., Matsunaga T., Hiyama E., Hayashi Y., Ando H., Suita S.,
RA Kaneko M., Sasaki F., Hashizume K., Ohnuma N., Nakagawara A.;
RT "Expression profiling and differential screening between hepatoblastomas
RT and the corresponding normal livers: identification of high expression of
RT the PLK1 oncogene as a poor-prognostic indicator of hepatoblastomas.";
RL Oncogene 23:5901-5911(2004).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX PubMed=16177791; DOI=10.1038/nature03983;
RA Nusbaum C., Zody M.C., Borowsky M.L., Kamal M., Kodira C.D., Taylor T.D.,
RA Whittaker C.A., Chang J.L., Cuomo C.A., Dewar K., FitzGerald M.G., Yang X.,
RA Abouelleil A., Allen N.R., Anderson S., Bloom T., Bugalter B., Butler J.,
RA Cook A., DeCaprio D., Engels R., Garber M., Gnirke A., Hafez N., Hall J.L.,
RA Norman C.H., Itoh T., Jaffe D.B., Kuroki Y., Lehoczky J., Lui A.,
RA Macdonald P., Mauceli E., Mikkelsen T.S., Naylor J.W., Nicol R., Nguyen C.,
RA Noguchi H., O'Leary S.B., Piqani B., Smith C.L., Talamas J.A., Topham K.,
RA Totoki Y., Toyoda A., Wain H.M., Young S.K., Zeng Q., Zimmer A.R.,
RA Fujiyama A., Hattori M., Birren B.W., Sakaki Y., Lander E.S.;
RT "DNA sequence and analysis of human chromosome 18.";
RL Nature 437:551-555(2005).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1; 2 AND 3).
RC TISSUE=Lung, Pancreas, and Testis;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 32-300 (ISOFORM 2).
RC TISSUE=Uterus;
RX PubMed=17974005; DOI=10.1186/1471-2164-8-399;
RA Bechtel S., Rosenfelder H., Duda A., Schmidt C.P., Ernst U.,
RA Wellenreuther R., Mehrle A., Schuster C., Bahr A., Bloecker H., Heubner D.,
RA Hoerlein A., Michel G., Wedler H., Koehrer K., Ottenwaelder B., Poustka A.,
RA Wiemann S., Schupp I.;
RT "The full-ORF clone resource of the German cDNA consortium.";
RL BMC Genomics 8:399-399(2007).
RN [6]
RP NUCLEOTIDE SEQUENCE [MRNA] OF 61-300 (ISOFORM 2), AND SUBCELLULAR LOCATION.
RX PubMed=15334068; DOI=10.1038/sj.onc.1207921;
RA Petroziello J., Yamane A., Westendorf L., Thompson M., McDonagh C.,
RA Cerveny C., Law C.-L., Wahl A., Carter P.;
RT "Suppression subtractive hybridization and expression profiling identifies
RT a unique set of genes overexpressed in non-small-cell lung cancer.";
RL Oncogene 23:7734-7745(2004).
RN [7]
RP FUNCTION, INTERACTION WITH CSTPP1 AND LRRC49, AND SUBCELLULAR LOCATION.
RX PubMed=34782749; DOI=10.1038/s41422-021-00584-9;
RA Wang L., Paudyal S.C., Kang Y., Owa M., Liang F.X., Spektor A., Knaut H.,
RA Sanchez I., Dynlacht B.D.;
RT "Regulators of tubulin polyglutamylation control nuclear shape and cilium
RT disassembly by balancing microtubule and actin assembly.";
RL Cell Res. 32:190-209(2022).
CC -!- FUNCTION: Subunit of the tubulin polyglutamylase complex (TPGC). The
CC complex mediates cilia and flagella polyglutamylation which is
CC essential for their biogenesis and motility.
CC {ECO:0000305|PubMed:34782749}.
CC -!- SUBUNIT: Part of the neuronal tubulin polyglutamylase complex which
CC contains TPGS1, TPGS2, TTLL1, LRRC49 and NICN1 (Probable). Interacts
CC with CSTPP1 and LRRC49 (PubMed:34782749). {ECO:0000269|PubMed:34782749,
CC ECO:0000305|PubMed:34782749}.
CC -!- INTERACTION:
CC Q68CL5; Q5XKR4: OTP; NbExp=3; IntAct=EBI-748091, EBI-12865884;
CC Q68CL5-3; P27797: CALR; NbExp=3; IntAct=EBI-9091010, EBI-1049597;
CC Q68CL5-3; P36957: DLST; NbExp=3; IntAct=EBI-9091010, EBI-351007;
CC Q68CL5-3; Q8TDX7: NEK7; NbExp=3; IntAct=EBI-9091010, EBI-1055945;
CC -!- SUBCELLULAR LOCATION: Cytoplasm, cytoskeleton
CC {ECO:0000269|PubMed:34782749}. Cytoplasm, cytoskeleton, microtubule
CC organizing center, centrosome, centriolar satellite
CC {ECO:0000269|PubMed:34782749}. Note=Associated with microtubules.
CC {ECO:0000269|PubMed:34782749}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=5;
CC Name=2;
CC IsoId=Q68CL5-2; Sequence=Displayed;
CC Name=1;
CC IsoId=Q68CL5-1; Sequence=VSP_020113;
CC Name=3;
CC IsoId=Q68CL5-3; Sequence=VSP_014884, VSP_014885;
CC Name=4;
CC IsoId=Q68CL5-4; Sequence=VSP_054525;
CC Name=5;
CC IsoId=Q68CL5-5; Sequence=VSP_055634;
CC -!- SEQUENCE CAUTION:
CC Sequence=AAH22199.2; Type=Erroneous initiation; Note=Truncated N-terminus.; Evidence={ECO:0000305};
CC Sequence=CAB43322.2; Type=Erroneous initiation; Note=Truncated N-terminus.; Evidence={ECO:0000305};
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DR EMBL; AK295817; BAG58634.1; -; mRNA.
DR EMBL; AB073382; BAD38633.1; -; mRNA.
DR EMBL; AC009854; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; AC090333; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; BC015178; AAH15178.2; -; mRNA.
DR EMBL; BC022199; AAH22199.2; ALT_INIT; mRNA.
DR EMBL; BC087845; AAH87845.1; -; mRNA.
DR EMBL; AL050225; CAB43322.2; ALT_INIT; mRNA.
DR EMBL; AY598336; AAT06747.1; -; mRNA.
DR CCDS; CCDS32817.1; -. [Q68CL5-2]
DR CCDS; CCDS62421.1; -. [Q68CL5-1]
DR CCDS; CCDS62422.1; -. [Q68CL5-4]
DR CCDS; CCDS62423.1; -. [Q68CL5-3]
DR CCDS; CCDS62424.1; -. [Q68CL5-5]
DR PIR; T08811; T08811.
DR RefSeq; NP_001258878.1; NM_001271949.1. [Q68CL5-4]
DR RefSeq; NP_001258879.1; NM_001271950.1. [Q68CL5-1]
DR RefSeq; NP_001258880.1; NM_001271951.1. [Q68CL5-5]
DR RefSeq; NP_001258881.1; NM_001271952.1.
DR RefSeq; NP_001258883.1; NM_001271954.1. [Q68CL5-3]
DR RefSeq; NP_056291.2; NM_015476.3. [Q68CL5-2]
DR AlphaFoldDB; Q68CL5; -.
DR BioGRID; 117438; 23.
DR IntAct; Q68CL5; 14.
DR STRING; 9606.ENSP00000464780; -.
DR iPTMnet; Q68CL5; -.
DR PhosphoSitePlus; Q68CL5; -.
DR BioMuta; TPGS2; -.
DR DMDM; 114152891; -.
DR EPD; Q68CL5; -.
DR jPOST; Q68CL5; -.
DR MassIVE; Q68CL5; -.
DR MaxQB; Q68CL5; -.
DR PaxDb; Q68CL5; -.
DR PeptideAtlas; Q68CL5; -.
DR PRIDE; Q68CL5; -.
DR ProteomicsDB; 66006; -. [Q68CL5-2]
DR ProteomicsDB; 66007; -. [Q68CL5-1]
DR ProteomicsDB; 66008; -. [Q68CL5-3]
DR Antibodypedia; 22336; 113 antibodies from 17 providers.
DR DNASU; 25941; -.
DR Ensembl; ENST00000334295.9; ENSP00000335144.3; ENSG00000134779.15. [Q68CL5-2]
DR Ensembl; ENST00000383056.7; ENSP00000372530.2; ENSG00000134779.15. [Q68CL5-1]
DR Ensembl; ENST00000589049.5; ENSP00000466973.1; ENSG00000134779.15. [Q68CL5-3]
DR Ensembl; ENST00000590842.5; ENSP00000464780.1; ENSG00000134779.15. [Q68CL5-5]
DR Ensembl; ENST00000593035.5; ENSP00000467392.1; ENSG00000134779.15. [Q68CL5-4]
DR GeneID; 25941; -.
DR KEGG; hsa:25941; -.
DR MANE-Select; ENST00000334295.9; ENSP00000335144.3; NM_015476.4; NP_056291.2.
DR UCSC; uc002kzw.3; human. [Q68CL5-2]
DR CTD; 25941; -.
DR DisGeNET; 25941; -.
DR GeneCards; TPGS2; -.
DR HGNC; HGNC:24561; TPGS2.
DR HPA; ENSG00000134779; Low tissue specificity.
DR neXtProt; NX_Q68CL5; -.
DR OpenTargets; ENSG00000134779; -.
DR PharmGKB; PA128394637; -.
DR VEuPathDB; HostDB:ENSG00000134779; -.
DR eggNOG; ENOG502R21Z; Eukaryota.
DR GeneTree; ENSGT00390000018344; -.
DR HOGENOM; CLU_079178_0_0_1; -.
DR InParanoid; Q68CL5; -.
DR OMA; FVLNWSY; -.
DR OrthoDB; 1286883at2759; -.
DR PhylomeDB; Q68CL5; -.
DR TreeFam; TF325540; -.
DR PathwayCommons; Q68CL5; -.
DR Reactome; R-HSA-8955332; Carboxyterminal post-translational modifications of tubulin.
DR SignaLink; Q68CL5; -.
DR BioGRID-ORCS; 25941; 8 hits in 1074 CRISPR screens.
DR ChiTaRS; TPGS2; human.
DR GenomeRNAi; 25941; -.
DR Pharos; Q68CL5; Tdark.
DR PRO; PR:Q68CL5; -.
DR Proteomes; UP000005640; Chromosome 18.
DR RNAct; Q68CL5; protein.
DR Bgee; ENSG00000134779; Expressed in ganglionic eminence and 198 other tissues.
DR ExpressionAtlas; Q68CL5; baseline and differential.
DR Genevisible; Q68CL5; HS.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-KW.
DR GO; GO:0005874; C:microtubule; IEA:UniProtKB-KW.
DR GO; GO:0005815; C:microtubule organizing center; IEA:UniProtKB-SubCell.
DR GO; GO:0018095; P:protein polyglutamylation; IEA:InterPro.
DR InterPro; IPR018958; Knr4/Smi1-like_dom.
DR InterPro; IPR037883; Knr4/Smi1-like_sf.
DR InterPro; IPR039231; TPGS2.
DR PANTHER; PTHR31854; PTHR31854; 1.
DR SMART; SM00860; SMI1_KNR4; 1.
DR SUPFAM; SSF160631; SSF160631; 1.
PE 1: Evidence at protein level;
KW Alternative splicing; Cytoplasm; Cytoskeleton; Microtubule;
KW Reference proteome.
FT CHAIN 1..300
FT /note="Tubulin polyglutamylase complex subunit 2"
FT /id="PRO_0000079304"
FT REGION 257..300
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 277..300
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT VAR_SEQ 85..127
FT /note="Missing (in isoform 1)"
FT /evidence="ECO:0000303|PubMed:15221005,
FT ECO:0000303|PubMed:15489334"
FT /id="VSP_020113"
FT VAR_SEQ 85..119
FT /note="Missing (in isoform 4)"
FT /evidence="ECO:0000303|PubMed:14702039"
FT /id="VSP_054525"
FT VAR_SEQ 220..300
FT /note="QWFSMYKPITYNTNLLTEETDSFVNKLDPSKVFKSKNKIVIPKKKGPVQPAG
FT GQKGPSGPSGPSTSSTSKSSSGSGNPTRK -> ITYNIKMNRRKCVHWKLAVEPLQWEL
FT RSCVSAGMGTGHKQRVSSMKPRSCSVCSRRQSNSMGTTVSQSFLSYGKNVLFFTVRCCF
FT (in isoform 5)"
FT /evidence="ECO:0000305"
FT /id="VSP_055634"
FT VAR_SEQ 220..227
FT /note="QWFSMYKP -> VREEHLPL (in isoform 3)"
FT /evidence="ECO:0000303|PubMed:15489334"
FT /id="VSP_014884"
FT VAR_SEQ 228..300
FT /note="Missing (in isoform 3)"
FT /evidence="ECO:0000303|PubMed:15489334"
FT /id="VSP_014885"
FT VARIANT 47
FT /note="R -> C (in dbSNP:rs2303507)"
FT /id="VAR_027410"
FT CONFLICT 44
FT /note="P -> S (in Ref. 5; CAB43322)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 300 AA; 33318 MW; F3AADD55882EC1E5 CRC64;
MEEEASSPGL GCSKPHLEKL TLGITRILES SPGVTEVTII EKPPAERHMI SSWEQKNNCV
MPEDVKNFYL MTNGFHMTWS VKLDEHIIPL GSMAINSISK LTQLTQSSMY SLPNAPTLAD
LEDDTHEASD DQPEKPHFDS RSVIFELDSC NGSGKVCLVY KSGKPALAED TEIWFLDRAL
YWHFLTDTFT AYYRLLITHL GLPQWQYAFT SYGISPQAKQ WFSMYKPITY NTNLLTEETD
SFVNKLDPSK VFKSKNKIVI PKKKGPVQPA GGQKGPSGPS GPSTSSTSKS SSGSGNPTRK
