ID   TPG_RANTE               Reviewed;          61 AA.
AC   P79875;
DT   01-NOV-1997, integrated into UniProtKB/Swiss-Prot.
DT   01-MAY-1997, sequence version 1.
DT   03-AUG-2022, entry version 67.
DE   RecName: Full=Temporin-1Tg {ECO:0000250|UniProtKB:P56917};
DE            Short=TG {ECO:0000250|UniProtKB:P56917};
DE   AltName: Full=Temporin-G {ECO:0000303|PubMed:22439858, ECO:0000303|PubMed:9022710};
DE   Flags: Precursor;
OS   Rana temporaria (European common frog).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Amphibia;
OC   Batrachia; Anura; Neobatrachia; Ranoidea; Ranidae; Rana; Rana.
OX   NCBI_TaxID=8407;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA], AMIDATION AT LEU-59, AND SUBCELLULAR LOCATION.
RC   TISSUE=Skin;
RX   PubMed=9022710; DOI=10.1111/j.1432-1033.1996.0788r.x;
RA   Simmaco M., Mignogna G., Canofeni S., Miele R., Mangoni M.L., Barra D.;
RT   "Temporins, antimicrobial peptides from the European red frog Rana
RT   temporaria.";
RL   Eur. J. Biochem. 242:788-792(1996).
RN   [2]
RP   FUNCTION.
RX   PubMed=22439858; DOI=10.1186/1472-6750-12-10;
RA   Ke T., Liang S., Huang J., Mao H., Chen J., Dong C., Huang J., Liu S.,
RA   Kang J., Liu D., Ma X.;
RT   "A novel PCR-based method for high throughput prokaryotic expression of
RT   antimicrobial peptide genes.";
RL   BMC Biotechnol. 12:10-10(2012).
RN   [3]
RP   FUNCTION AS INSULINOTROPIC PEPTIDE, AND BIOASSAY.
RX   PubMed=29349894; DOI=10.1002/psc.3065;
RA   Musale V., Casciaro B., Mangoni M.L., Abdel-Wahab Y.H.A., Flatt P.R.,
RA   Conlon J.M.;
RT   "Assessment of the potential of temporin peptides from the frog Rana
RT   temporaria (Ranidae) as anti-diabetic agents.";
RL   J. Pept. Sci. 24:1-12(2018).
CC   -!- FUNCTION: Amphipathic alpha-helical antimicrobial peptide with activity
CC       against Gram-positive bacteria (M.luteus), Gram-negative bacteria
CC       (E.coli), and fungi (S.cerevisiae) (PubMed:22439858). Stimulates
CC       insulin release from pancreatic beta-cells in a dose-dependent manner,
CC       without increasing intracellular calcium (PubMed:29349894). Does not
CC       protects BRIN-BD11 beta-cells against cytokine-induced apoptosis
CC       (PubMed:29349894). In vivo, intraperitoneal injection together with a
CC       glucose load into mice improves glucose tolerance with a concomitant
CC       increase in insulin secretion (PubMed:29349894).
CC       {ECO:0000269|PubMed:22439858, ECO:0000269|PubMed:29349894}.
CC   -!- SUBCELLULAR LOCATION: Secreted {ECO:0000269|PubMed:9022710}.
CC   -!- TISSUE SPECIFICITY: Expressed by the skin glands.
CC       {ECO:0000305|PubMed:9022710}.
CC   -!- SIMILARITY: Belongs to the frog skin active peptide (FSAP) family.
CC       Temporin subfamily. {ECO:0000305}.
CC   -!- WEB RESOURCE: Name=The antimicrobial peptide database;
CC       URL="https://wangapd3.com/database/query_output.php?ID=00099";
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DR   EMBL; Y09395; CAA70564.1; -; mRNA.
DR   AlphaFoldDB; P79875; -.
DR   TCDB; 1.C.52.1.5; the dermaseptin (dermaseptin) family.
DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR   GO; GO:0050830; P:defense response to Gram-positive bacterium; IEA:UniProt.
DR   GO; GO:0045087; P:innate immune response; IEA:UniProtKB-KW.
DR   InterPro; IPR004275; Frog_antimicrobial_propeptide.
DR   Pfam; PF03032; FSAP_sig_propep; 1.
PE   1: Evidence at protein level;
KW   Amidation; Amphibian defense peptide; Antibiotic; Antimicrobial;
KW   Cleavage on pair of basic residues; Immunity; Innate immunity; Secreted;
KW   Signal.
FT   SIGNAL          1..22
FT                   /evidence="ECO:0000255"
FT   PROPEP          23..44
FT                   /evidence="ECO:0000305|PubMed:9022710"
FT                   /id="PRO_0000003473"
FT   PEPTIDE         47..59
FT                   /note="Temporin-1Tg"
FT                   /evidence="ECO:0000269|PubMed:9022710"
FT                   /id="PRO_0000003474"
FT   MOD_RES         59
FT                   /note="Leucine amide"
FT                   /evidence="ECO:0000269|PubMed:9022710"
SQ   SEQUENCE   61 AA;  7171 MW;  EDF5A8BC79DFD9F2 CRC64;
     MFTLKKSLLL LFFLGTINLS LCEEERDADE ERRDDLEERD VEVEKRFFPV IGRILNGILG
     K