ID   TPH_SYLGU               Reviewed;          13 AA.
AC   P84858;
DT   09-JAN-2007, integrated into UniProtKB/Swiss-Prot.
DT   09-JAN-2007, sequence version 1.
DT   17-JUN-2020, entry version 20.
DE   RecName: Full=Temporin-GH;
DE   AltName: Full=AMP-5;
OS   Sylvirana guentheri (Gunther's frog) (Rana guentheri).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Amphibia;
OC   Batrachia; Anura; Neobatrachia; Ranoidea; Ranidae; Sylvirana.
OX   NCBI_TaxID=110109;
RN   [1] {ECO:0000305}
RP   PROTEIN SEQUENCE, FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, MASS
RP   SPECTROMETRY, AND AMIDATION AT LEU-13.
RC   TISSUE=Skin secretion {ECO:0000269|PubMed:16979798};
RX   PubMed=16979798; DOI=10.1016/j.peptides.2006.08.007;
RA   Zhou J., McClean S., Thompson A., Zhang Y., Shaw C., Rao P., Bjourson A.J.;
RT   "Purification and characterization of novel antimicrobial peptides from the
RT   skin secretion of Hylarana guentheri.";
RL   Peptides 27:3077-3084(2006).
CC   -!- FUNCTION: Antimicrobial peptide. Active against the Gram-positive
CC       bacteria S.aureus FDA209P (MIC=44.3 ug/ml) and B.subtilis ATCC 6633
CC       (MIC>64 ug/ml), but not active against the Gram-negative bacterium
CC       E.coli or the fungus C.albicans. {ECO:0000269|PubMed:16979798}.
CC   -!- SUBCELLULAR LOCATION: Secreted {ECO:0000269|PubMed:16979798}.
CC   -!- TISSUE SPECIFICITY: Expressed by the skin glands.
CC       {ECO:0000269|PubMed:16979798}.
CC   -!- MASS SPECTROMETRY: Mass=1438.8; Method=Electrospray;
CC       Evidence={ECO:0000269|PubMed:16979798};
CC   -!- SIMILARITY: Belongs to the frog skin active peptide (FSAP) family.
CC       Temporin subfamily. {ECO:0000305}.
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DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR   GO; GO:0042742; P:defense response to bacterium; IEA:UniProtKB-KW.
PE   1: Evidence at protein level;
KW   Amidation; Amphibian defense peptide; Antibiotic; Antimicrobial;
KW   Direct protein sequencing; Secreted.
FT   PEPTIDE         1..13
FT                   /note="Temporin-GH"
FT                   /evidence="ECO:0000269|PubMed:16979798"
FT                   /id="PRO_0000271188"
FT   MOD_RES         13
FT                   /note="Leucine amide"
FT                   /evidence="ECO:0000269|PubMed:16979798"
SQ   SEQUENCE   13 AA;  1441 MW;  D70518BB9A83D879 CRC64;
     FLPLLFGAIS HLL