ID   TPI2_GIAIN              Reviewed;         257 AA.
AC   P36187;
DT   01-JUN-1994, integrated into UniProtKB/Swiss-Prot.
DT   01-JUN-1994, sequence version 1.
DT   03-AUG-2022, entry version 85.
DE   RecName: Full=Triosephosphate isomerase;
DE            Short=TIM;
DE            EC=5.3.1.1;
DE   AltName: Full=Triose-phosphate isomerase;
OS   Giardia intestinalis (Giardia lamblia).
OC   Eukaryota; Metamonada; Diplomonadida; Hexamitidae; Giardiinae; Giardia.
OX   NCBI_TaxID=5741;
RN   [1]
RP   NUCLEOTIDE SEQUENCE.
RX   PubMed=8299763; DOI=10.1006/expr.1994.1008;
RA   Mowatt M.R., Weinbach E.C., Howard T.C., Nash T.E.;
RT   "Complementation of an Escherichia coli glycolysis mutant by Giardia
RT   lamblia triosephosphate isomerase.";
RL   Exp. Parasitol. 78:85-92(1994).
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=D-glyceraldehyde 3-phosphate = dihydroxyacetone phosphate;
CC         Xref=Rhea:RHEA:18585, ChEBI:CHEBI:57642, ChEBI:CHEBI:59776;
CC         EC=5.3.1.1;
CC   -!- PATHWAY: Carbohydrate biosynthesis; gluconeogenesis.
CC   -!- PATHWAY: Carbohydrate degradation; glycolysis; D-glyceraldehyde 3-
CC       phosphate from glycerone phosphate: step 1/1.
CC   -!- SUBUNIT: Homodimer. {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the triosephosphate isomerase family.
CC       {ECO:0000305}.
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DR   EMBL; L02116; AAA18205.1; -; Unassigned_DNA.
DR   AlphaFoldDB; P36187; -.
DR   SMR; P36187; -.
DR   VEuPathDB; GiardiaDB:DHA2_93938; -.
DR   VEuPathDB; GiardiaDB:GL50581_1369; -.
DR   VEuPathDB; GiardiaDB:GL50803_0093938; -.
DR   UniPathway; UPA00109; UER00189.
DR   UniPathway; UPA00138; -.
DR   GO; GO:0004807; F:triose-phosphate isomerase activity; IEA:UniProtKB-EC.
DR   GO; GO:0006094; P:gluconeogenesis; IEA:UniProtKB-UniPathway.
DR   GO; GO:0006096; P:glycolytic process; IEA:UniProtKB-UniPathway.
DR   CDD; cd00311; TIM; 1.
DR   Gene3D; 3.20.20.70; -; 1.
DR   HAMAP; MF_00147_B; TIM_B; 1.
DR   InterPro; IPR013785; Aldolase_TIM.
DR   InterPro; IPR035990; TIM_sf.
DR   InterPro; IPR022896; TrioseP_Isoase_bac/euk.
DR   InterPro; IPR000652; Triosephosphate_isomerase.
DR   InterPro; IPR020861; Triosephosphate_isomerase_AS.
DR   PANTHER; PTHR21139; PTHR21139; 1.
DR   Pfam; PF00121; TIM; 1.
DR   SUPFAM; SSF51351; SSF51351; 1.
DR   TIGRFAMs; TIGR00419; tim; 1.
DR   PROSITE; PS00171; TIM_1; 1.
DR   PROSITE; PS51440; TIM_2; 1.
PE   3: Inferred from homology;
KW   Gluconeogenesis; Glycolysis; Isomerase.
FT   CHAIN           1..257
FT                   /note="Triosephosphate isomerase"
FT                   /id="PRO_0000090136"
FT   ACT_SITE        96
FT                   /note="Electrophile"
FT                   /evidence="ECO:0000250"
FT   ACT_SITE        170
FT                   /note="Proton acceptor"
FT                   /evidence="ECO:0000250"
FT   BINDING         11
FT                   /ligand="substrate"
FT                   /evidence="ECO:0000250"
FT   BINDING         13
FT                   /ligand="substrate"
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   257 AA;  27851 MW;  B23006F46E5BE1C6 CRC64;
     MPARRPFIGG NFKCNGSLDF IKSHVASIAS YKIPESVDVV VAPSFVHLST AIAANTSKCL
     KIAAQNVYLE GNGAWTGETS VEMLLDMGLS HVIIGHSERR RIMGETNEQS AKKAKRALDK
     GMTVIFCTGE TLDERKANNT MEVNIAQLEA LKKEIGESKK LWENVVIAYE PVWSIGTGVV
     ATPEQAEEVH VGLRKWFAEK VCAEGAQHIR IIYGGSANGS NCEKLGQCPN IDGFLVGGAS
     LKPEFTTMID ILAKTRA