ID   TPIS_CULTA              Reviewed;         247 AA.
AC   P30741;
DT   01-APR-1993, integrated into UniProtKB/Swiss-Prot.
DT   23-JAN-2007, sequence version 2.
DT   03-AUG-2022, entry version 96.
DE   RecName: Full=Triosephosphate isomerase;
DE            Short=TIM;
DE            EC=5.3.1.1;
DE   AltName: Full=Triose-phosphate isomerase;
GN   Name=Tpi;
OS   Culex tarsalis (Encephalitis mosquito).
OC   Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC   Neoptera; Endopterygota; Diptera; Nematocera; Culicoidea; Culicidae;
OC   Culicinae; Culicini; Culex; Culex.
OX   NCBI_TaxID=7177;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND PROTEIN SEQUENCE OF 2-27.
RX   PubMed=8429888; DOI=10.1038/361470a0;
RA   Tittiger C., Whyard S., Walker V.K.;
RT   "A novel intron site in the triosephosphate isomerase gene from the
RT   mosquito Culex tarsalis.";
RL   Nature 361:470-472(1993).
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=D-glyceraldehyde 3-phosphate = dihydroxyacetone phosphate;
CC         Xref=Rhea:RHEA:18585, ChEBI:CHEBI:57642, ChEBI:CHEBI:59776;
CC         EC=5.3.1.1;
CC   -!- PATHWAY: Carbohydrate biosynthesis; gluconeogenesis.
CC   -!- PATHWAY: Carbohydrate degradation; glycolysis; D-glyceraldehyde 3-
CC       phosphate from glycerone phosphate: step 1/1.
CC   -!- SUBUNIT: Homodimer.
CC   -!- SIMILARITY: Belongs to the triosephosphate isomerase family.
CC       {ECO:0000305}.
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DR   EMBL; L07390; AAA73976.1; -; Genomic_DNA.
DR   PIR; S29716; S29716.
DR   AlphaFoldDB; P30741; -.
DR   SMR; P30741; -.
DR   PRIDE; P30741; -.
DR   UniPathway; UPA00109; UER00189.
DR   UniPathway; UPA00138; -.
DR   GO; GO:0004807; F:triose-phosphate isomerase activity; IEA:UniProtKB-EC.
DR   GO; GO:0006094; P:gluconeogenesis; IEA:UniProtKB-UniPathway.
DR   GO; GO:0006096; P:glycolytic process; IEA:UniProtKB-UniPathway.
DR   CDD; cd00311; TIM; 1.
DR   Gene3D; 3.20.20.70; -; 1.
DR   HAMAP; MF_00147_B; TIM_B; 1.
DR   InterPro; IPR013785; Aldolase_TIM.
DR   InterPro; IPR035990; TIM_sf.
DR   InterPro; IPR022896; TrioseP_Isoase_bac/euk.
DR   InterPro; IPR000652; Triosephosphate_isomerase.
DR   InterPro; IPR020861; Triosephosphate_isomerase_AS.
DR   PANTHER; PTHR21139; PTHR21139; 1.
DR   Pfam; PF00121; TIM; 1.
DR   SUPFAM; SSF51351; SSF51351; 1.
DR   TIGRFAMs; TIGR00419; tim; 1.
DR   PROSITE; PS00171; TIM_1; 1.
DR   PROSITE; PS51440; TIM_2; 1.
PE   1: Evidence at protein level;
KW   Direct protein sequencing; Gluconeogenesis; Glycolysis; Isomerase.
FT   INIT_MET        1
FT                   /note="Removed"
FT                   /evidence="ECO:0000269|PubMed:8429888"
FT   CHAIN           2..247
FT                   /note="Triosephosphate isomerase"
FT                   /id="PRO_0000090129"
FT   ACT_SITE        94
FT                   /note="Electrophile"
FT                   /evidence="ECO:0000250"
FT   ACT_SITE        164
FT                   /note="Proton acceptor"
FT                   /evidence="ECO:0000250"
FT   BINDING         10
FT                   /ligand="substrate"
FT                   /evidence="ECO:0000250"
FT   BINDING         12
FT                   /ligand="substrate"
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   247 AA;  26389 MW;  F5B84AB1765D9022 CRC64;
     MGRKFCVGGN WKMNGDKASI ADLCKVLTTG PLNADTEVVV GCPAPYLTLA RSQLPDSVCV
     AAQNCYKVPK GAFTGEISPA MLKDLNIGWV ILGHSERRAI FGESDELIAD KVAHALAEGL
     KVIACIGETL QEREAGQTEA VCFRQTKAIA DKVKDWSNVV IAYEPVWAIG TGKTASPEQA
     QEVHAALRKW FTENVSADVS AAIRIQYGGS VTAANCRELA AKPDIDGFLV GGASLKPEFI
     QIVNARQ