ID   TPIS_DROSI              Reviewed;         247 AA.
AC   Q7JNS1; O76996;
DT   10-MAY-2005, integrated into UniProtKB/Swiss-Prot.
DT   05-JUL-2004, sequence version 1.
DT   03-AUG-2022, entry version 81.
DE   RecName: Full=Triosephosphate isomerase;
DE            Short=TIM;
DE            EC=5.3.1.1;
DE   AltName: Full=Triose-phosphate isomerase;
GN   Name=Tpi;
OS   Drosophila simulans (Fruit fly).
OC   Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC   Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea;
OC   Drosophilidae; Drosophila; Sophophora.
OX   NCBI_TaxID=7240;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=Ds-Ma, Ds-Oa, Ds-Ot, Ds-Ra, Ds-RB, Ds-Te, Ds-Wa, and DsDPF;
RX   PubMed=9615457; DOI=10.1093/oxfordjournals.molbev.a025979;
RA   Hasson E., Wang I.-N., Zeng L.-W., Kreitman M., Eanes W.F.;
RT   "Nucleotide variation in the triosephosphate isomerase (Tpi) locus of
RT   Drosophila melanogaster and D. simulans.";
RL   Mol. Biol. Evol. 15:756-769(1998).
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=D-glyceraldehyde 3-phosphate = dihydroxyacetone phosphate;
CC         Xref=Rhea:RHEA:18585, ChEBI:CHEBI:57642, ChEBI:CHEBI:59776;
CC         EC=5.3.1.1;
CC   -!- PATHWAY: Carbohydrate biosynthesis; gluconeogenesis.
CC   -!- PATHWAY: Carbohydrate degradation; glycolysis; D-glyceraldehyde 3-
CC       phosphate from glycerone phosphate: step 1/1.
CC   -!- SUBUNIT: Homodimer.
CC   -!- SIMILARITY: Belongs to the triosephosphate isomerase family.
CC       {ECO:0000305}.
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DR   EMBL; U60861; AAC39066.1; -; Genomic_DNA.
DR   EMBL; U60862; AAC39067.1; -; Genomic_DNA.
DR   EMBL; U60863; AAC39068.1; -; Genomic_DNA.
DR   EMBL; U60864; AAC39069.1; -; Genomic_DNA.
DR   EMBL; U60865; AAC39070.1; -; Genomic_DNA.
DR   EMBL; U60866; AAC39071.1; -; Genomic_DNA.
DR   EMBL; U60867; AAC39072.1; -; Genomic_DNA.
DR   EMBL; U60868; AAC39073.1; -; Genomic_DNA.
DR   EMBL; U60869; AAC39074.1; -; Genomic_DNA.
DR   RefSeq; XP_016036938.1; XM_016174069.1.
DR   AlphaFoldDB; Q7JNS1; -.
DR   SMR; Q7JNS1; -.
DR   PRIDE; Q7JNS1; -.
DR   EnsemblMetazoa; FBtr0352644; FBpp0317196; FBgn0025034.
DR   GeneID; 6730181; -.
DR   UniPathway; UPA00109; UER00189.
DR   UniPathway; UPA00138; -.
DR   Bgee; FBgn0025034; Expressed in adult organism and 3 other tissues.
DR   GO; GO:0004807; F:triose-phosphate isomerase activity; ISS:UniProtKB.
DR   GO; GO:0006094; P:gluconeogenesis; IEA:UniProtKB-UniPathway.
DR   GO; GO:0019682; P:glyceraldehyde-3-phosphate metabolic process; ISS:UniProtKB.
DR   GO; GO:0006096; P:glycolytic process; IEA:UniProtKB-UniPathway.
DR   CDD; cd00311; TIM; 1.
DR   Gene3D; 3.20.20.70; -; 1.
DR   HAMAP; MF_00147_B; TIM_B; 1.
DR   InterPro; IPR013785; Aldolase_TIM.
DR   InterPro; IPR035990; TIM_sf.
DR   InterPro; IPR022896; TrioseP_Isoase_bac/euk.
DR   InterPro; IPR000652; Triosephosphate_isomerase.
DR   InterPro; IPR020861; Triosephosphate_isomerase_AS.
DR   PANTHER; PTHR21139; PTHR21139; 1.
DR   Pfam; PF00121; TIM; 1.
DR   SUPFAM; SSF51351; SSF51351; 1.
DR   TIGRFAMs; TIGR00419; tim; 1.
DR   PROSITE; PS00171; TIM_1; 1.
DR   PROSITE; PS51440; TIM_2; 1.
PE   3: Inferred from homology;
KW   Gluconeogenesis; Glycolysis; Isomerase.
FT   CHAIN           1..247
FT                   /note="Triosephosphate isomerase"
FT                   /id="PRO_0000090131"
FT   ACT_SITE        94
FT                   /note="Electrophile"
FT                   /evidence="ECO:0000250"
FT   ACT_SITE        164
FT                   /note="Proton acceptor"
FT                   /evidence="ECO:0000250"
FT   BINDING         10
FT                   /ligand="substrate"
FT                   /evidence="ECO:0000250"
FT   BINDING         12
FT                   /ligand="substrate"
FT                   /evidence="ECO:0000250"
FT   VARIANT         142
FT                   /note="V -> M (in strain: Ds-NA)"
SQ   SEQUENCE   247 AA;  26626 MW;  68D7C0269A80C3CB CRC64;
     MSRKFCVGGN WKMNGDQKSI AEIAKTLSSA ALDPNTEVVI GCPAIYLMYA RNLLPCELGL
     AGQNAYKVAK GAFTGEISPA MLKDIGADWV ILGHSERRAI FGESDALIAE KAEHALAEGL
     KVIACIGETL EEREAGKTNE VVARQMCAYA QKIKDWKNVV VAYEPVWAIG TGKTATPDQA
     QEVHAFLRQW LSDNISKEVS ASLRIQYGGS VTAANAKELA KKPDIDGFLV GGASLKPEFV
     DIINARQ