ID   TPIS_HELVI              Reviewed;         215 AA.
AC   P55275;
DT   01-OCT-1996, integrated into UniProtKB/Swiss-Prot.
DT   01-OCT-1996, sequence version 1.
DT   25-MAY-2022, entry version 83.
DE   RecName: Full=Triosephosphate isomerase;
DE            Short=TIM;
DE            EC=5.3.1.1;
DE   AltName: Full=Triose-phosphate isomerase;
DE   Flags: Fragment;
GN   Name=Tpi;
OS   Heliothis virescens (Tobacco budworm moth).
OC   Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC   Neoptera; Endopterygota; Lepidoptera; Glossata; Ditrysia; Noctuoidea;
OC   Noctuidae; Heliothinae; Heliothis.
OX   NCBI_TaxID=7102;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=7667320; DOI=10.1073/pnas.92.18.8507;
RA   Logsdon J.M. Jr., Tyshenko M.G., Dixon C., D-Jafari J., Walker V.K.,
RA   Palmer J.D.;
RT   "Seven newly discovered intron positions in the triose-phosphate isomerase
RT   gene: evidence for the introns-late theory.";
RL   Proc. Natl. Acad. Sci. U.S.A. 92:8507-8511(1995).
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=D-glyceraldehyde 3-phosphate = dihydroxyacetone phosphate;
CC         Xref=Rhea:RHEA:18585, ChEBI:CHEBI:57642, ChEBI:CHEBI:59776;
CC         EC=5.3.1.1;
CC   -!- PATHWAY: Carbohydrate biosynthesis; gluconeogenesis.
CC   -!- PATHWAY: Carbohydrate degradation; glycolysis; D-glyceraldehyde 3-
CC       phosphate from glycerone phosphate: step 1/1.
CC   -!- SUBUNIT: Homodimer. {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the triosephosphate isomerase family.
CC       {ECO:0000305}.
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DR   EMBL; U23080; AAA79847.1; -; Genomic_DNA.
DR   AlphaFoldDB; P55275; -.
DR   SMR; P55275; -.
DR   UniPathway; UPA00109; UER00189.
DR   UniPathway; UPA00138; -.
DR   GO; GO:0004807; F:triose-phosphate isomerase activity; IEA:UniProtKB-EC.
DR   GO; GO:0006094; P:gluconeogenesis; IEA:UniProtKB-UniPathway.
DR   GO; GO:0006096; P:glycolytic process; IEA:UniProtKB-UniPathway.
DR   CDD; cd00311; TIM; 1.
DR   Gene3D; 3.20.20.70; -; 1.
DR   HAMAP; MF_00147_B; TIM_B; 1.
DR   InterPro; IPR013785; Aldolase_TIM.
DR   InterPro; IPR035990; TIM_sf.
DR   InterPro; IPR022896; TrioseP_Isoase_bac/euk.
DR   InterPro; IPR000652; Triosephosphate_isomerase.
DR   InterPro; IPR020861; Triosephosphate_isomerase_AS.
DR   PANTHER; PTHR21139; PTHR21139; 1.
DR   Pfam; PF00121; TIM; 1.
DR   SUPFAM; SSF51351; SSF51351; 1.
DR   TIGRFAMs; TIGR00419; tim; 1.
DR   PROSITE; PS00171; TIM_1; 1.
DR   PROSITE; PS51440; TIM_2; 1.
PE   3: Inferred from homology;
KW   Gluconeogenesis; Glycolysis; Isomerase.
FT   CHAIN           <1..>215
FT                   /note="Triosephosphate isomerase"
FT                   /id="PRO_0000090134"
FT   ACT_SITE        82
FT                   /note="Electrophile"
FT                   /evidence="ECO:0000250"
FT   ACT_SITE        153
FT                   /note="Proton acceptor"
FT                   /evidence="ECO:0000250"
FT   NON_TER         1
FT   NON_TER         215
SQ   SEQUENCE   215 AA;  22933 MW;  06BA0B731AFFD031 CRC64;
     MNGDKKQVTD IVETLKKGPL DPNVEVVIGV PAIYLAYVQS IVPGTISVAA QNCWKVAKGA
     FTGEISPAMI KDIGANWVIL GHSERRTIFG EKDDLVAEKV AHALENGLKV IACIGETLEE
     REAGKTEEVV FRQTKALLPA IGNNWANVVL AYEPVWAIGT GKTASPQQAQ DVHASLRNWL
     SSNASPDVAA SVRIQYGGSV TAANAKELSA FPDID