TPIS_MAIZE
ID TPIS_MAIZE Reviewed; 253 AA.
AC P12863;
DT 01-OCT-1989, integrated into UniProtKB/Swiss-Prot.
DT 23-JAN-2007, sequence version 3.
DT 03-AUG-2022, entry version 137.
DE RecName: Full=Triosephosphate isomerase, cytosolic;
DE Short=TIM;
DE Short=Triose-phosphate isomerase;
DE EC=5.3.1.1;
OS Zea mays (Maize).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; PACMAD clade;
OC Panicoideae; Andropogonodae; Andropogoneae; Tripsacinae; Zea.
OX NCBI_TaxID=4577;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX PubMed=3755078; DOI=10.1016/0092-8674(86)90867-6;
RA Marchionni M., Gilbert W.;
RT "The triosephosphate isomerase gene from maize: introns antedate the plant-
RT animal divergence.";
RL Cell 46:133-141(1986).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=D-glyceraldehyde 3-phosphate = dihydroxyacetone phosphate;
CC Xref=Rhea:RHEA:18585, ChEBI:CHEBI:57642, ChEBI:CHEBI:59776;
CC EC=5.3.1.1;
CC -!- PATHWAY: Carbohydrate biosynthesis; gluconeogenesis.
CC -!- PATHWAY: Carbohydrate degradation; glycolysis; D-glyceraldehyde 3-
CC phosphate from glycerone phosphate: step 1/1.
CC -!- SUBUNIT: Homodimer.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000305}.
CC -!- MISCELLANEOUS: In plants, there are two types of TPIS, cytosolic and
CC plastid.
CC -!- SIMILARITY: Belongs to the triosephosphate isomerase family.
CC {ECO:0000305}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; D00012; BAA00009.1; -; Genomic_DNA.
DR EMBL; L00371; AAB81110.1; -; Genomic_DNA.
DR PIR; A25501; ISZMT.
DR RefSeq; NP_001140424.1; NM_001146952.1.
DR PDB; 6CG9; X-ray; 1.80 A; A/B=1-253.
DR PDBsum; 6CG9; -.
DR AlphaFoldDB; P12863; -.
DR SMR; P12863; -.
DR STRING; 4577.GRMZM2G030784_P02; -.
DR PaxDb; P12863; -.
DR PRIDE; P12863; -.
DR EnsemblPlants; Zm00001eb124410_T001; Zm00001eb124410_P001; Zm00001eb124410.
DR GeneID; 100272481; -.
DR Gramene; Zm00001eb124410_T001; Zm00001eb124410_P001; Zm00001eb124410.
DR KEGG; zma:100272481; -.
DR MaizeGDB; 65619; -.
DR eggNOG; KOG1643; Eukaryota.
DR HOGENOM; CLU_024251_2_0_1; -.
DR OMA; VWAIGEH; -.
DR OrthoDB; 1272577at2759; -.
DR UniPathway; UPA00109; UER00189.
DR UniPathway; UPA00138; -.
DR Proteomes; UP000007305; Chromosome 3.
DR ExpressionAtlas; P12863; baseline and differential.
DR Genevisible; P12863; ZM.
DR GO; GO:0005829; C:cytosol; IBA:GO_Central.
DR GO; GO:0004807; F:triose-phosphate isomerase activity; IBA:GO_Central.
DR GO; GO:0006094; P:gluconeogenesis; IBA:GO_Central.
DR GO; GO:0046166; P:glyceraldehyde-3-phosphate biosynthetic process; IBA:GO_Central.
DR GO; GO:0019563; P:glycerol catabolic process; IBA:GO_Central.
DR GO; GO:0006096; P:glycolytic process; IBA:GO_Central.
DR CDD; cd00311; TIM; 1.
DR Gene3D; 3.20.20.70; -; 1.
DR HAMAP; MF_00147_B; TIM_B; 1.
DR InterPro; IPR013785; Aldolase_TIM.
DR InterPro; IPR035990; TIM_sf.
DR InterPro; IPR022896; TrioseP_Isoase_bac/euk.
DR InterPro; IPR000652; Triosephosphate_isomerase.
DR InterPro; IPR020861; Triosephosphate_isomerase_AS.
DR PANTHER; PTHR21139; PTHR21139; 1.
DR Pfam; PF00121; TIM; 1.
DR SUPFAM; SSF51351; SSF51351; 1.
DR TIGRFAMs; TIGR00419; tim; 1.
DR PROSITE; PS00171; TIM_1; 1.
DR PROSITE; PS51440; TIM_2; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Cytoplasm; Gluconeogenesis; Glycolysis; Isomerase;
KW Reference proteome.
FT INIT_MET 1
FT /note="Removed"
FT CHAIN 2..253
FT /note="Triosephosphate isomerase, cytosolic"
FT /id="PRO_0000090149"
FT ACT_SITE 96
FT /note="Electrophile"
FT /evidence="ECO:0000250"
FT ACT_SITE 166
FT /note="Proton acceptor"
FT /evidence="ECO:0000250"
FT BINDING 10
FT /ligand="substrate"
FT /evidence="ECO:0000250"
FT BINDING 12
FT /ligand="substrate"
FT /evidence="ECO:0000250"
FT STRAND 5..10
FT /evidence="ECO:0007829|PDB:6CG9"
FT HELIX 17..29
FT /evidence="ECO:0007829|PDB:6CG9"
FT TURN 35..37
FT /evidence="ECO:0007829|PDB:6CG9"
FT STRAND 38..43
FT /evidence="ECO:0007829|PDB:6CG9"
FT HELIX 46..48
FT /evidence="ECO:0007829|PDB:6CG9"
FT HELIX 49..55
FT /evidence="ECO:0007829|PDB:6CG9"
FT STRAND 60..65
FT /evidence="ECO:0007829|PDB:6CG9"
FT HELIX 81..87
FT /evidence="ECO:0007829|PDB:6CG9"
FT STRAND 91..95
FT /evidence="ECO:0007829|PDB:6CG9"
FT HELIX 97..102
FT /evidence="ECO:0007829|PDB:6CG9"
FT HELIX 107..119
FT /evidence="ECO:0007829|PDB:6CG9"
FT STRAND 123..128
FT /evidence="ECO:0007829|PDB:6CG9"
FT HELIX 132..136
FT /evidence="ECO:0007829|PDB:6CG9"
FT HELIX 140..152
FT /evidence="ECO:0007829|PDB:6CG9"
FT STRAND 161..165
FT /evidence="ECO:0007829|PDB:6CG9"
FT HELIX 168..170
FT /evidence="ECO:0007829|PDB:6CG9"
FT STRAND 171..174
FT /evidence="ECO:0007829|PDB:6CG9"
FT HELIX 179..196
FT /evidence="ECO:0007829|PDB:6CG9"
FT HELIX 199..204
FT /evidence="ECO:0007829|PDB:6CG9"
FT STRAND 207..209
FT /evidence="ECO:0007829|PDB:6CG9"
FT TURN 215..217
FT /evidence="ECO:0007829|PDB:6CG9"
FT HELIX 218..222
FT /evidence="ECO:0007829|PDB:6CG9"
FT STRAND 229..232
FT /evidence="ECO:0007829|PDB:6CG9"
FT HELIX 234..237
FT /evidence="ECO:0007829|PDB:6CG9"
FT HELIX 240..245
FT /evidence="ECO:0007829|PDB:6CG9"
FT HELIX 246..249
FT /evidence="ECO:0007829|PDB:6CG9"
SQ SEQUENCE 253 AA; 27025 MW; C5FCEC8DB59B0543 CRC64;
MGRKFFVGGN WKCNGTTDQV EKIVKTLNEG QVPPSDVVEV VVSPPYVFLP VVKSQLRQEF
HVAAQNCWVK KGGAFTGEVS AEMLVNLGVP WVILGHSERR ALLGESNEFV GDKVAYALSQ
GLKVIACVGE TLEQREAGST MDVVAAQTKA IAEKIKDWSN VVVAYEPVWA IGTGKVATPA
QAQEVHASLR DWLKTNASPE VAESTRIIYG GSVTAANCKE LAAQPDVDGF LVGGASLKPE
FIDIINAATV KSA
