ID   TPIS_PLAOI              Reviewed;          86 AA.
AC   P85814;
DT   01-JUL-2008, integrated into UniProtKB/Swiss-Prot.
DT   01-JUL-2008, sequence version 1.
DT   25-MAY-2022, entry version 42.
DE   RecName: Full=Triosephosphate isomerase;
DE            Short=TIM;
DE            EC=5.3.1.1;
DE   AltName: Full=Triose-phosphate isomerase;
DE   AltName: Allergen=Pla o 4;
DE   Flags: Fragments;
OS   Platanus orientalis (Oriental plane-tree).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; Proteales; Platanaceae; Platanus.
OX   NCBI_TaxID=122832;
RN   [1] {ECO:0000305}
RP   PROTEIN SEQUENCE, MASS SPECTROMETRY, AND ALLERGEN.
RC   TISSUE=Pollen {ECO:0000269|Ref.1};
RA   Pazouki N.;
RT   "Identification, cloning and expression of Platanus orientalis pollen
RT   allergens.";
RL   Thesis (2008), Islamic Azad university, Iran.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=D-glyceraldehyde 3-phosphate = dihydroxyacetone phosphate;
CC         Xref=Rhea:RHEA:18585, ChEBI:CHEBI:57642, ChEBI:CHEBI:59776;
CC         EC=5.3.1.1; Evidence={ECO:0000250|UniProtKB:P04789,
CC         ECO:0000255|PROSITE-ProRule:PRU10127};
CC   -!- PATHWAY: Carbohydrate biosynthesis; gluconeogenesis.
CC       {ECO:0000250|UniProtKB:P04789, ECO:0000255|PROSITE-ProRule:PRU10127}.
CC   -!- PATHWAY: Carbohydrate degradation; glycolysis; D-glyceraldehyde 3-
CC       phosphate from glycerone phosphate: step 1/1.
CC       {ECO:0000250|UniProtKB:P04789, ECO:0000255|PROSITE-ProRule:PRU10127}.
CC   -!- SUBUNIT: Homodimer. {ECO:0000250|UniProtKB:P04789, ECO:0000255|PROSITE-
CC       ProRule:PRU10127}.
CC   -!- MASS SPECTROMETRY: Mass=27074.8; Method=MALDI;
CC       Evidence={ECO:0000269|Ref.1};
CC   -!- ALLERGEN: Causes an allergic reaction in human. {ECO:0000269|Ref.1}.
CC   -!- SIMILARITY: Belongs to the triosephosphate isomerase family.
CC       {ECO:0000255|PROSITE-ProRule:PRU10127}.
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DR   AlphaFoldDB; P85814; -.
DR   SMR; P85814; -.
DR   UniPathway; UPA00109; UER00189.
DR   UniPathway; UPA00138; -.
DR   GO; GO:0004807; F:triose-phosphate isomerase activity; IEA:UniProtKB-EC.
DR   GO; GO:0006094; P:gluconeogenesis; IEA:UniProtKB-UniPathway.
DR   GO; GO:0006096; P:glycolytic process; IEA:UniProtKB-UniPathway.
DR   Gene3D; 3.20.20.70; -; 2.
DR   InterPro; IPR013785; Aldolase_TIM.
DR   InterPro; IPR035990; TIM_sf.
DR   InterPro; IPR000652; Triosephosphate_isomerase.
DR   InterPro; IPR020861; Triosephosphate_isomerase_AS.
DR   PANTHER; PTHR21139; PTHR21139; 2.
DR   Pfam; PF00121; TIM; 2.
DR   SUPFAM; SSF51351; SSF51351; 1.
DR   PROSITE; PS00171; TIM_1; 1.
DR   PROSITE; PS51440; TIM_2; 1.
PE   1: Evidence at protein level;
KW   Allergen; Direct protein sequencing; Gluconeogenesis; Glycolysis;
KW   Isomerase.
FT   CHAIN           <1..>86
FT                   /note="Triosephosphate isomerase"
FT                   /id="PRO_0000343452"
FT   ACT_SITE        62
FT                   /note="Proton acceptor"
FT                   /evidence="ECO:0000250|UniProtKB:P04789,
FT                   ECO:0000255|PROSITE-ProRule:PRU10127"
FT   NON_CONS        16..17
FT                   /evidence="ECO:0000303|Ref.1"
FT   NON_CONS        29..30
FT                   /evidence="ECO:0000303|Ref.1"
FT   NON_CONS        47..48
FT                   /evidence="ECO:0000303|Ref.1"
FT   NON_CONS        56..57
FT                   /evidence="ECO:0000303|Ref.1"
FT   NON_TER         1
FT                   /evidence="ECO:0000303|Ref.1"
FT   NON_TER         86
FT                   /evidence="ECO:0000303|Ref.1"
SQ   SEQUENCE   86 AA;  8935 MW;  D816B8C38C23ACA1 CRC64;
     MAATSLTAPP SFSGLRASRA VVAMAGTGKG GAFTGEISVE QLKDIGRWVI LGHSERIVVA
     YEPVWAIGTG KVATPDQAQE VHGLCR