ID   BTUDA_HALS3             Reviewed;         398 AA.
AC   B0R5G4;
DT   31-MAY-2011, integrated into UniProtKB/Swiss-Prot.
DT   08-APR-2008, sequence version 1.
DT   03-AUG-2022, entry version 81.
DE   RecName: Full=Cobalamin import ATP-binding protein BtuD;
DE            EC=7.6.2.8;
DE   AltName: Full=Vitamin B12-transporting ATPase;
GN   Name=btuD; OrderedLocusNames=OE_2955F;
OS   Halobacterium salinarum (strain ATCC 29341 / DSM 671 / R1).
OC   Archaea; Euryarchaeota; Stenosarchaea group; Halobacteria; Halobacteriales;
OC   Halobacteriaceae; Halobacterium.
OX   NCBI_TaxID=478009;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 29341 / DSM 671 / R1;
RX   PubMed=18313895; DOI=10.1016/j.ygeno.2008.01.001;
RA   Pfeiffer F., Schuster S.C., Broicher A., Falb M., Palm P., Rodewald K.,
RA   Ruepp A., Soppa J., Tittor J., Oesterhelt D.;
RT   "Evolution in the laboratory: the genome of Halobacterium salinarum strain
RT   R1 compared to that of strain NRC-1.";
RL   Genomics 91:335-346(2008).
CC   -!- FUNCTION: Required for corrinoid utilization. Probably part of the ABC
CC       transporter complex BtuCDF involved in cobalamin (vitamin B12) import.
CC       Probably responsible for energy coupling to the transport system (By
CC       similarity). {ECO:0000250}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=an R-cob(III)alamin(out) + ATP + H2O = ADP + an R-
CC         cob(III)alamin(in) + H(+) + phosphate; Xref=Rhea:RHEA:17873,
CC         ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:30616,
CC         ChEBI:CHEBI:43474, ChEBI:CHEBI:140785, ChEBI:CHEBI:456216;
CC         EC=7.6.2.8;
CC   -!- SUBUNIT: The complex is composed of two ATP-binding proteins (BtuD),
CC       two transmembrane proteins (BtuC) and a solute-binding protein (BtuF).
CC       {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000250}; Peripheral membrane
CC       protein {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the ABC transporter superfamily. {ECO:0000305}.
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DR   EMBL; AM774415; CAP13981.1; -; Genomic_DNA.
DR   RefSeq; WP_010902995.1; NC_010364.1.
DR   AlphaFoldDB; B0R5G4; -.
DR   SMR; B0R5G4; -.
DR   EnsemblBacteria; CAP13981; CAP13981; OE_2955F.
DR   GeneID; 5953596; -.
DR   KEGG; hsl:OE_2955F; -.
DR   HOGENOM; CLU_000604_0_0_2; -.
DR   OMA; KNFFEMS; -.
DR   PhylomeDB; B0R5G4; -.
DR   Proteomes; UP000001321; Chromosome.
DR   GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0015420; F:ABC-type vitamin B12 transporter activity; IEA:UniProtKB-EC.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR   Gene3D; 3.40.50.300; -; 1.
DR   InterPro; IPR003593; AAA+_ATPase.
DR   InterPro; IPR003439; ABC_transporter-like_ATP-bd.
DR   InterPro; IPR017871; ABC_transporter-like_CS.
DR   InterPro; IPR027417; P-loop_NTPase.
DR   Pfam; PF00005; ABC_tran; 1.
DR   SMART; SM00382; AAA; 1.
DR   SUPFAM; SSF52540; SSF52540; 1.
DR   PROSITE; PS00211; ABC_TRANSPORTER_1; 1.
DR   PROSITE; PS50893; ABC_TRANSPORTER_2; 1.
PE   3: Inferred from homology;
KW   ATP-binding; Cell membrane; Membrane; Nucleotide-binding; Translocase;
KW   Transport.
FT   CHAIN           1..398
FT                   /note="Cobalamin import ATP-binding protein BtuD"
FT                   /id="PRO_0000408970"
FT   DOMAIN          3..237
FT                   /note="ABC transporter"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00434"
FT   BINDING         35..42
FT                   /ligand="ATP"
FT                   /ligand_id="ChEBI:CHEBI:30616"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00434"
SQ   SEQUENCE   398 AA;  40466 MW;  524C614B8196F48A CRC64;
     MTLDVTGLDV ELAGTRILDD VHASIRDGHL VGVVGPNGAG KSTLLRAMNG LITPTAGTVL
     VAGDDVHALS SAAASRRIAT VPQDASVSFE FTVRQVVEMG RHPHTTRFGT DTDTAVVDRA
     MARTGVAQFA ARDVTSLSGG ERQRVLLARA LAQAAPVLLL DEPTASLDVN HQIRTLEVVR
     DLADSEDRAV VAAIHDLDLA ARYCDELVVV ADGRVHDAGA PRSVLTPDTI RAAFDARVAV
     GTDPATGAVT VTPLPDRTSA AADTSVHVVG GGDSATPVVR RLVSAGASVS VGPVVEGDTD
     HETARRVGCP CTSVAPFTRL EDTTAASATR ADIAAADVIA VPVAAAARPG VRGLLTGAVP
     TLAVGDAAGA PEWADRLVAC DAVVSAVGAL ADTPSDGV