TPIS_STELP
ID TPIS_STELP Reviewed; 257 AA.
AC P48497;
DT 01-FEB-1996, integrated into UniProtKB/Swiss-Prot.
DT 01-FEB-1996, sequence version 1.
DT 03-AUG-2022, entry version 91.
DE RecName: Full=Triosephosphate isomerase, cytosolic;
DE Short=TIM;
DE Short=Triose-phosphate isomerase;
DE EC=5.3.1.1;
GN Name=TPI;
OS Stellaria longipes (Longstalk starwort) (Alsine longipes).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC Caryophyllales; Caryophyllaceae; Alsineae; Stellaria.
OX NCBI_TaxID=19744;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RC STRAIN=cv. Goldie; TISSUE=Leaf;
RX PubMed=8181735; DOI=10.1139/g94-019;
RA Zhang X.-H., Chinnappa C.C.;
RT "Triose phosphate isomerase of Stellaria longipes (Caryophyllaceae).";
RL Genome 37:148-156(1994).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=D-glyceraldehyde 3-phosphate = dihydroxyacetone phosphate;
CC Xref=Rhea:RHEA:18585, ChEBI:CHEBI:57642, ChEBI:CHEBI:59776;
CC EC=5.3.1.1;
CC -!- PATHWAY: Carbohydrate biosynthesis; gluconeogenesis.
CC -!- PATHWAY: Carbohydrate degradation; glycolysis; D-glyceraldehyde 3-
CC phosphate from glycerone phosphate: step 1/1.
CC -!- SUBUNIT: Homodimer. {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000305}.
CC -!- TISSUE SPECIFICITY: Higher levels found in leaves than in roots.
CC -!- MISCELLANEOUS: In plants, there are two types of TPIS, cytosolic and
CC plastid.
CC -!- SIMILARITY: Belongs to the triosephosphate isomerase family.
CC {ECO:0000305}.
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DR EMBL; S70730; AAB30759.1; -; mRNA.
DR AlphaFoldDB; P48497; -.
DR SMR; P48497; -.
DR PRIDE; P48497; -.
DR UniPathway; UPA00109; UER00189.
DR UniPathway; UPA00138; -.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0004807; F:triose-phosphate isomerase activity; IEA:UniProtKB-EC.
DR GO; GO:0006094; P:gluconeogenesis; IEA:UniProtKB-UniPathway.
DR GO; GO:0006096; P:glycolytic process; IEA:UniProtKB-UniPathway.
DR CDD; cd00311; TIM; 1.
DR Gene3D; 3.20.20.70; -; 1.
DR HAMAP; MF_00147_B; TIM_B; 1.
DR InterPro; IPR013785; Aldolase_TIM.
DR InterPro; IPR035990; TIM_sf.
DR InterPro; IPR022896; TrioseP_Isoase_bac/euk.
DR InterPro; IPR000652; Triosephosphate_isomerase.
DR InterPro; IPR020861; Triosephosphate_isomerase_AS.
DR PANTHER; PTHR21139; PTHR21139; 1.
DR Pfam; PF00121; TIM; 1.
DR SUPFAM; SSF51351; SSF51351; 1.
DR TIGRFAMs; TIGR00419; tim; 1.
DR PROSITE; PS00171; TIM_1; 1.
DR PROSITE; PS51440; TIM_2; 1.
PE 2: Evidence at transcript level;
KW Cytoplasm; Gluconeogenesis; Glycolysis; Isomerase.
FT CHAIN 1..257
FT /note="Triosephosphate isomerase, cytosolic"
FT /id="PRO_0000090155"
FT ACT_SITE 96
FT /note="Electrophile"
FT /evidence="ECO:0000250"
FT ACT_SITE 167
FT /note="Proton acceptor"
FT /evidence="ECO:0000250"
FT BINDING 10
FT /ligand="substrate"
FT /evidence="ECO:0000250"
FT BINDING 12
FT /ligand="substrate"
FT /evidence="ECO:0000250"
SQ SEQUENCE 257 AA; 27499 MW; FF55463129BB08A0 CRC64;
MGRKFFVGGN WKCNGTQESV SKIVDTLNEP TIAANDVVTV VVSPPYVFLP DENAELKHEI
QVAAQNCWVK KGGAFTGEVS AQMLANLGIT WVILGHSERR TLLGESNEFV GKKAAYAQSE
GLGVIACIGE LLEEREAGKT FDVCFKQLKS FARFPAKPWD NVVVAYEPVW AIGTGKVASP
EQAQEVHVAV RDWLKTNVSE EVASKTRIIY GGSVNGGNSL ALAAQEDVDG FLVGGASLKG
PEFATIINSV TAKKVAA