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TPK1_MOUSE
ID   TPK1_MOUSE              Reviewed;         243 AA.
AC   Q9R0M5; Q3UWB5; Q8CAB5; Q8CEE8; Q8R1Q6;
DT   01-MAR-2004, integrated into UniProtKB/Swiss-Prot.
DT   01-MAY-2000, sequence version 1.
DT   03-AUG-2022, entry version 156.
DE   RecName: Full=Thiamin pyrophosphokinase 1 {ECO:0000303|PubMed:10567383};
DE            Short=mTPK1 {ECO:0000303|PubMed:10567383};
DE            EC=2.7.6.2 {ECO:0000269|PubMed:10567383};
DE   AltName: Full=Thiamine pyrophosphokinase 1;
GN   Name=Tpk1 {ECO:0000303|PubMed:10567383, ECO:0000312|MGI:MGI:1352500};
OS   Mus musculus (Mouse).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC   Murinae; Mus; Mus.
OX   NCBI_TaxID=10090;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), FUNCTION, SUBUNIT, TISSUE
RP   SPECIFICITY, AND CATALYTIC ACTIVITY.
RC   TISSUE=Embryo;
RX   PubMed=10567383; DOI=10.1074/jbc.274.48.34129;
RA   Nosaka K., Onozuka M., Nishino H., Nishimura H., Kawasaki Y., Ueyama H.;
RT   "Molecular cloning and expression of a mouse thiamin pyrophosphokinase
RT   cDNA.";
RL   J. Biol. Chem. 274:34129-34133(1999).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1; 2 AND 3).
RC   STRAIN=C57BL/6J; TISSUE=Colon, Embryo, Embryonic liver, and Hypothalamus;
RX   PubMed=16141072; DOI=10.1126/science.1112014;
RA   Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N.,
RA   Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K.,
RA   Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.,
RA   Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R.,
RA   Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T.,
RA   Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A.,
RA   Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B.,
RA   Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M.,
RA   Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S.,
RA   Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E.,
RA   Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D.,
RA   Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M.,
RA   Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H.,
RA   Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V.,
RA   Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S.,
RA   Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H.,
RA   Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N.,
RA   Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F.,
RA   Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G.,
RA   Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z.,
RA   Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C.,
RA   Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y.,
RA   Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S.,
RA   Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K.,
RA   Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R.,
RA   van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H.,
RA   Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M.,
RA   Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C.,
RA   Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S.,
RA   Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K.,
RA   Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M.,
RA   Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C.,
RA   Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A.,
RA   Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.;
RT   "The transcriptional landscape of the mammalian genome.";
RL   Science 309:1559-1563(2005).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
RC   TISSUE=Kidney;
RX   PubMed=15489334; DOI=10.1101/gr.2596504;
RG   The MGC Project Team;
RT   "The status, quality, and expansion of the NIH full-length cDNA project:
RT   the Mammalian Gene Collection (MGC).";
RL   Genome Res. 14:2121-2127(2004).
RN   [4]
RP   IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC   TISSUE=Brain, Kidney, Liver, Lung, Pancreas, and Spleen;
RX   PubMed=21183079; DOI=10.1016/j.cell.2010.12.001;
RA   Huttlin E.L., Jedrychowski M.P., Elias J.E., Goswami T., Rad R.,
RA   Beausoleil S.A., Villen J., Haas W., Sowa M.E., Gygi S.P.;
RT   "A tissue-specific atlas of mouse protein phosphorylation and expression.";
RL   Cell 143:1174-1189(2010).
RN   [5]
RP   X-RAY CRYSTALLOGRAPHY (1.9 ANGSTROMS) OF ISOFORM 1 IN COMPLEX WITH
RP   THIAMINE, AND HOMODIMERIZATION.
RX   PubMed=11419946; DOI=10.1006/jmbi.2001.4727;
RA   Timm D.E., Liu J., Baker L.-J., Harris R.A.;
RT   "Crystal structure of thiamin pyrophosphokinase.";
RL   J. Mol. Biol. 310:195-204(2001).
RN   [6]
RP   X-RAY CRYSTALLOGRAPHY (2.5 ANGSTROMS) OF ISOFORM 2 IN COMPLEX WITH
RP   PYRITHIAMINE PYROPHOSPHATE AND AMP, AND FUNCTION.
RX   PubMed=16365036; DOI=10.1074/jbc.m510951200;
RA   Liu J.Y., Timm D.E., Hurley T.D.;
RT   "Pyrithiamine as a substrate for thiamine pyrophosphokinase.";
RL   J. Biol. Chem. 281:6601-6607(2006).
CC   -!- FUNCTION: Catalyzes the phosphorylation of thiamine to thiamine
CC       pyrophosphate. Can also catalyze the phosphorylation of pyrithiamine to
CC       pyrithiamine pyrophosphate. {ECO:0000269|PubMed:10567383,
CC       ECO:0000269|PubMed:16365036}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=ATP + thiamine = AMP + H(+) + thiamine diphosphate;
CC         Xref=Rhea:RHEA:11576, ChEBI:CHEBI:15378, ChEBI:CHEBI:18385,
CC         ChEBI:CHEBI:30616, ChEBI:CHEBI:58937, ChEBI:CHEBI:456215; EC=2.7.6.2;
CC         Evidence={ECO:0000269|PubMed:10567383};
CC   -!- PATHWAY: Cofactor biosynthesis; thiamine diphosphate biosynthesis;
CC       thiamine diphosphate from thiamine: step 1/1.
CC       {ECO:0000305|PubMed:10567383}.
CC   -!- SUBUNIT: Homodimer. {ECO:0000269|PubMed:10567383,
CC       ECO:0000269|PubMed:11419946, ECO:0000269|PubMed:16365036}.
CC   -!- ALTERNATIVE PRODUCTS:
CC       Event=Alternative splicing; Named isoforms=3;
CC       Name=1;
CC         IsoId=Q9R0M5-1; Sequence=Displayed;
CC       Name=2;
CC         IsoId=Q9R0M5-2; Sequence=VSP_009597;
CC       Name=3;
CC         IsoId=Q9R0M5-3; Sequence=VSP_009595, VSP_009596;
CC   -!- TISSUE SPECIFICITY: Detected in kidney and liver, and at lower levels
CC       in heart, brain and testis. {ECO:0000269|PubMed:10567383}.
CC   -!- MISCELLANEOUS: [Isoform 3]: May be due to intron retention.
CC       {ECO:0000305}.
CC   -!- SIMILARITY: Belongs to the thiamine pyrophosphokinase family.
CC       {ECO:0000305}.
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DR   EMBL; AB027568; BAA87040.1; -; mRNA.
DR   EMBL; AK028431; BAC25948.1; -; mRNA.
DR   EMBL; AK035044; BAC28923.1; -; mRNA.
DR   EMBL; AK039131; BAC30248.1; -; mRNA.
DR   EMBL; AK136486; BAE23001.1; -; mRNA.
DR   EMBL; BC015246; AAH15246.1; -; mRNA.
DR   EMBL; BC023354; AAH23354.1; -; mRNA.
DR   CCDS; CCDS39474.1; -. [Q9R0M5-1]
DR   CCDS; CCDS80529.1; -. [Q9R0M5-2]
DR   RefSeq; NP_001298040.1; NM_001311111.1. [Q9R0M5-2]
DR   RefSeq; NP_038889.1; NM_013861.4. [Q9R0M5-1]
DR   PDB; 1IG3; X-ray; 1.90 A; A/B=1-243.
DR   PDB; 2F17; X-ray; 2.50 A; A/B=1-243.
DR   PDBsum; 1IG3; -.
DR   PDBsum; 2F17; -.
DR   AlphaFoldDB; Q9R0M5; -.
DR   SMR; Q9R0M5; -.
DR   STRING; 10090.ENSMUSP00000065631; -.
DR   PhosphoSitePlus; Q9R0M5; -.
DR   EPD; Q9R0M5; -.
DR   jPOST; Q9R0M5; -.
DR   MaxQB; Q9R0M5; -.
DR   PaxDb; Q9R0M5; -.
DR   PeptideAtlas; Q9R0M5; -.
DR   PRIDE; Q9R0M5; -.
DR   ProteomicsDB; 297506; -. [Q9R0M5-1]
DR   ProteomicsDB; 297507; -. [Q9R0M5-2]
DR   ProteomicsDB; 297508; -. [Q9R0M5-3]
DR   Antibodypedia; 18523; 182 antibodies from 24 providers.
DR   DNASU; 29807; -.
DR   Ensembl; ENSMUST00000067888; ENSMUSP00000065631; ENSMUSG00000029735. [Q9R0M5-1]
DR   Ensembl; ENSMUST00000114644; ENSMUSP00000110291; ENSMUSG00000029735. [Q9R0M5-2]
DR   GeneID; 29807; -.
DR   KEGG; mmu:29807; -.
DR   UCSC; uc009bsq.1; mouse. [Q9R0M5-1]
DR   UCSC; uc009bsr.1; mouse. [Q9R0M5-2]
DR   CTD; 27010; -.
DR   MGI; MGI:1352500; Tpk1.
DR   VEuPathDB; HostDB:ENSMUSG00000029735; -.
DR   eggNOG; KOG3153; Eukaryota.
DR   GeneTree; ENSGT00390000016016; -.
DR   HOGENOM; CLU_044237_0_1_1; -.
DR   InParanoid; Q9R0M5; -.
DR   OMA; HHLYMMT; -.
DR   OrthoDB; 1428589at2759; -.
DR   PhylomeDB; Q9R0M5; -.
DR   TreeFam; TF313224; -.
DR   Reactome; R-MMU-196819; Vitamin B1 (thiamin) metabolism.
DR   UniPathway; UPA00060; UER00597.
DR   BioGRID-ORCS; 29807; 6 hits in 74 CRISPR screens.
DR   ChiTaRS; Tpk1; mouse.
DR   EvolutionaryTrace; Q9R0M5; -.
DR   PRO; PR:Q9R0M5; -.
DR   Proteomes; UP000000589; Chromosome 6.
DR   RNAct; Q9R0M5; protein.
DR   Bgee; ENSMUSG00000029735; Expressed in right kidney and 158 other tissues.
DR   Genevisible; Q9R0M5; MM.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR   GO; GO:0042802; F:identical protein binding; ISO:MGI.
DR   GO; GO:0016301; F:kinase activity; IEA:UniProtKB-KW.
DR   GO; GO:0030975; F:thiamine binding; IEA:InterPro.
DR   GO; GO:0004788; F:thiamine diphosphokinase activity; IDA:MGI.
DR   GO; GO:0016310; P:phosphorylation; IEA:UniProtKB-KW.
DR   GO; GO:0009229; P:thiamine diphosphate biosynthetic process; ISO:MGI.
DR   GO; GO:0006772; P:thiamine metabolic process; IDA:MGI.
DR   CDD; cd07995; TPK; 1.
DR   Gene3D; 3.40.50.10240; -; 1.
DR   InterPro; IPR006282; Thi_PPkinase.
DR   InterPro; IPR016966; Thiamin_pyrophosphokinase_euk.
DR   InterPro; IPR007373; Thiamin_PyroPKinase_B1-bd.
DR   InterPro; IPR036371; TPK_B1-bd_sf.
DR   InterPro; IPR007371; TPK_catalytic.
DR   InterPro; IPR036759; TPK_catalytic_sf.
DR   Pfam; PF04265; TPK_B1_binding; 1.
DR   Pfam; PF04263; TPK_catalytic; 1.
DR   PIRSF; PIRSF031057; Thiamin_pyrophosphokinase; 1.
DR   SMART; SM00983; TPK_B1_binding; 1.
DR   SUPFAM; SSF63862; SSF63862; 1.
DR   SUPFAM; SSF63999; SSF63999; 1.
DR   TIGRFAMs; TIGR01378; thi_PPkinase; 1.
PE   1: Evidence at protein level;
KW   3D-structure; Alternative splicing; ATP-binding; Kinase;
KW   Nucleotide-binding; Reference proteome; Transferase.
FT   CHAIN           1..243
FT                   /note="Thiamin pyrophosphokinase 1"
FT                   /id="PRO_0000072648"
FT   VAR_SEQ         39..63
FT                   /note="ALLRACADGGANHLYDLTEGERESF -> VLGKKSQEVLAERRLIEPLGIQS
FT                   SL (in isoform 3)"
FT                   /evidence="ECO:0000303|PubMed:16141072"
FT                   /id="VSP_009595"
FT   VAR_SEQ         64..243
FT                   /note="Missing (in isoform 3)"
FT                   /evidence="ECO:0000303|PubMed:16141072"
FT                   /id="VSP_009596"
FT   VAR_SEQ         119..167
FT                   /note="Missing (in isoform 2)"
FT                   /evidence="ECO:0000303|PubMed:15489334,
FT                   ECO:0000303|PubMed:16141072"
FT                   /id="VSP_009597"
FT   CONFLICT        20
FT                   /note="C -> W (in Ref. 2; BAC25948)"
FT                   /evidence="ECO:0000305"
FT   STRAND          3..5
FT                   /evidence="ECO:0007829|PDB:1IG3"
FT   HELIX           9..11
FT                   /evidence="ECO:0007829|PDB:1IG3"
FT   STRAND          12..14
FT                   /evidence="ECO:0007829|PDB:1IG3"
FT   STRAND          19..23
FT                   /evidence="ECO:0007829|PDB:1IG3"
FT   HELIX           32..38
FT                   /evidence="ECO:0007829|PDB:1IG3"
FT   STRAND          40..45
FT                   /evidence="ECO:0007829|PDB:1IG3"
FT   HELIX           48..54
FT                   /evidence="ECO:0007829|PDB:1IG3"
FT   HELIX           60..62
FT                   /evidence="ECO:0007829|PDB:1IG3"
FT   STRAND          66..70
FT                   /evidence="ECO:0007829|PDB:1IG3"
FT   STRAND          72..75
FT                   /evidence="ECO:0007829|PDB:2F17"
FT   HELIX           77..85
FT                   /evidence="ECO:0007829|PDB:1IG3"
FT   STRAND          89..92
FT                   /evidence="ECO:0007829|PDB:1IG3"
FT   HELIX           100..114
FT                   /evidence="ECO:0007829|PDB:1IG3"
FT   STRAND          120..125
FT                   /evidence="ECO:0007829|PDB:1IG3"
FT   STRAND          128..130
FT                   /evidence="ECO:0007829|PDB:1IG3"
FT   HELIX           132..144
FT                   /evidence="ECO:0007829|PDB:1IG3"
FT   HELIX           145..147
FT                   /evidence="ECO:0007829|PDB:1IG3"
FT   STRAND          153..157
FT                   /evidence="ECO:0007829|PDB:1IG3"
FT   STRAND          160..165
FT                   /evidence="ECO:0007829|PDB:1IG3"
FT   STRAND          167..173
FT                   /evidence="ECO:0007829|PDB:1IG3"
FT   STRAND          179..186
FT                   /evidence="ECO:0007829|PDB:1IG3"
FT   STRAND          192..202
FT                   /evidence="ECO:0007829|PDB:1IG3"
FT   STRAND          205..210
FT                   /evidence="ECO:0007829|PDB:1IG3"
FT   TURN            211..213
FT                   /evidence="ECO:0007829|PDB:1IG3"
FT   STRAND          216..219
FT                   /evidence="ECO:0007829|PDB:1IG3"
FT   STRAND          223..233
FT                   /evidence="ECO:0007829|PDB:1IG3"
FT   STRAND          235..241
FT                   /evidence="ECO:0007829|PDB:1IG3"
SQ   SEQUENCE   243 AA;  27068 MW;  946896D2493F44EA CRC64;
     MEHAFTPLEP LLPTGNLKYC LVVLNQPLDA RFRHLWKKAL LRACADGGAN HLYDLTEGER
     ESFLPEFVSG DFDSIRPEVK EYYTKKGCDL ISTPDQDHTD FTKCLQVLQR KIEEKELQVD
     VIVTLGGLGG RFDQIMASVN TLFQATHITP VPIIIIQKDS LIYLLQPGKH RLHVDTGMEG
     SWCGLIPVGQ PCNQVTTTGL KWNLTNDVLG FGTLVSTSNT YDGSGLVTVE TDHPLLWTMA
     IKS
 
 
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