ID   TPK3_ORYSJ              Reviewed;         267 AA.
AC   Q60DX1; A0A0P0WLG7; B9FP75;
DT   16-OCT-2013, integrated into UniProtKB/Swiss-Prot.
DT   23-NOV-2004, sequence version 1.
DT   03-AUG-2022, entry version 106.
DE   RecName: Full=Thiamine pyrophosphokinase 3;
DE            Short=OsTPK3;
DE            EC=2.7.6.2;
DE   AltName: Full=Thiamine kinase 3;
GN   Name=TPK3; OrderedLocusNames=Os05g0367400, LOC_Os05g30454;
GN   ORFNames=OsJ_18271, OSJNBa0090H02.18;
OS   Oryza sativa subsp. japonica (Rice).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; BOP clade;
OC   Oryzoideae; Oryzeae; Oryzinae; Oryza; Oryza sativa.
OX   NCBI_TaxID=39947;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=cv. Nipponbare;
RX   PubMed=16261349; DOI=10.1007/s00438-005-0039-y;
RA   Cheng C.-H., Chung M.C., Liu S.-M., Chen S.-K., Kao F.Y., Lin S.-J.,
RA   Hsiao S.-H., Tseng I.C., Hsing Y.-I.C., Wu H.-P., Chen C.-S., Shaw J.-F.,
RA   Wu J., Matsumoto T., Sasaki T., Chen H.-C., Chow T.-Y.;
RT   "A fine physical map of the rice chromosome 5.";
RL   Mol. Genet. Genomics 274:337-345(2005).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=cv. Nipponbare;
RX   PubMed=16100779; DOI=10.1038/nature03895;
RG   International rice genome sequencing project (IRGSP);
RT   "The map-based sequence of the rice genome.";
RL   Nature 436:793-800(2005).
RN   [3]
RP   GENOME REANNOTATION.
RC   STRAIN=cv. Nipponbare;
RX   PubMed=18089549; DOI=10.1093/nar/gkm978;
RG   The rice annotation project (RAP);
RT   "The rice annotation project database (RAP-DB): 2008 update.";
RL   Nucleic Acids Res. 36:D1028-D1033(2008).
RN   [4]
RP   GENOME REANNOTATION.
RC   STRAIN=cv. Nipponbare;
RX   PubMed=24280374; DOI=10.1186/1939-8433-6-4;
RA   Kawahara Y., de la Bastide M., Hamilton J.P., Kanamori H., McCombie W.R.,
RA   Ouyang S., Schwartz D.C., Tanaka T., Wu J., Zhou S., Childs K.L.,
RA   Davidson R.M., Lin H., Quesada-Ocampo L., Vaillancourt B., Sakai H.,
RA   Lee S.S., Kim J., Numa H., Itoh T., Buell C.R., Matsumoto T.;
RT   "Improvement of the Oryza sativa Nipponbare reference genome using next
RT   generation sequence and optical map data.";
RL   Rice 6:4-4(2013).
RN   [5]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=cv. Nipponbare;
RX   PubMed=15685292; DOI=10.1371/journal.pbio.0030038;
RA   Yu J., Wang J., Lin W., Li S., Li H., Zhou J., Ni P., Dong W., Hu S.,
RA   Zeng C., Zhang J., Zhang Y., Li R., Xu Z., Li S., Li X., Zheng H., Cong L.,
RA   Lin L., Yin J., Geng J., Li G., Shi J., Liu J., Lv H., Li J., Wang J.,
RA   Deng Y., Ran L., Shi X., Wang X., Wu Q., Li C., Ren X., Wang J., Wang X.,
RA   Li D., Liu D., Zhang X., Ji Z., Zhao W., Sun Y., Zhang Z., Bao J., Han Y.,
RA   Dong L., Ji J., Chen P., Wu S., Liu J., Xiao Y., Bu D., Tan J., Yang L.,
RA   Ye C., Zhang J., Xu J., Zhou Y., Yu Y., Zhang B., Zhuang S., Wei H.,
RA   Liu B., Lei M., Yu H., Li Y., Xu H., Wei S., He X., Fang L., Zhang Z.,
RA   Zhang Y., Huang X., Su Z., Tong W., Li J., Tong Z., Li S., Ye J., Wang L.,
RA   Fang L., Lei T., Chen C.-S., Chen H.-C., Xu Z., Li H., Huang H., Zhang F.,
RA   Xu H., Li N., Zhao C., Li S., Dong L., Huang Y., Li L., Xi Y., Qi Q.,
RA   Li W., Zhang B., Hu W., Zhang Y., Tian X., Jiao Y., Liang X., Jin J.,
RA   Gao L., Zheng W., Hao B., Liu S.-M., Wang W., Yuan L., Cao M.,
RA   McDermott J., Samudrala R., Wang J., Wong G.K.-S., Yang H.;
RT   "The genomes of Oryza sativa: a history of duplications.";
RL   PLoS Biol. 3:266-281(2005).
RN   [6]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   STRAIN=cv. Nipponbare;
RX   PubMed=12869764; DOI=10.1126/science.1081288;
RG   The rice full-length cDNA consortium;
RT   "Collection, mapping, and annotation of over 28,000 cDNA clones from
RT   japonica rice.";
RL   Science 301:376-379(2003).
CC   -!- FUNCTION: Catalyzes the phosphorylation of thiamine to thiamine
CC       pyrophosphate (TPP). TPP is an active cofactor for enzymes involved in
CC       glycolysis and energy production. Plant leaves require high levels of
CC       TPP for photosynthesis and carbohydrate metabolism (By similarity).
CC       {ECO:0000250}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=ATP + thiamine = AMP + H(+) + thiamine diphosphate;
CC         Xref=Rhea:RHEA:11576, ChEBI:CHEBI:15378, ChEBI:CHEBI:18385,
CC         ChEBI:CHEBI:30616, ChEBI:CHEBI:58937, ChEBI:CHEBI:456215; EC=2.7.6.2;
CC   -!- PATHWAY: Cofactor biosynthesis; thiamine diphosphate biosynthesis;
CC       thiamine diphosphate from thiamine: step 1/1.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm, cytosol {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the thiamine pyrophosphokinase family.
CC       {ECO:0000305}.
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DR   EMBL; AC137618; AAV31365.1; -; Genomic_DNA.
DR   EMBL; AP008211; BAF17251.1; -; Genomic_DNA.
DR   EMBL; AP014961; BAS93654.1; -; Genomic_DNA.
DR   EMBL; CM000142; EEE63458.1; -; Genomic_DNA.
DR   EMBL; AK108439; BAG98403.1; -; mRNA.
DR   RefSeq; XP_015637762.1; XM_015782276.1.
DR   AlphaFoldDB; Q60DX1; -.
DR   SMR; Q60DX1; -.
DR   STRING; 4530.OS05T0367400-01; -.
DR   PaxDb; Q60DX1; -.
DR   PRIDE; Q60DX1; -.
DR   EnsemblPlants; Os05t0367400-01; Os05t0367400-01; Os05g0367400.
DR   GeneID; 4338566; -.
DR   Gramene; Os05t0367400-01; Os05t0367400-01; Os05g0367400.
DR   KEGG; osa:4338566; -.
DR   eggNOG; KOG3153; Eukaryota.
DR   HOGENOM; CLU_044237_0_2_1; -.
DR   InParanoid; Q60DX1; -.
DR   OMA; RPTMRHS; -.
DR   OrthoDB; 1428589at2759; -.
DR   UniPathway; UPA00060; UER00597.
DR   Proteomes; UP000000763; Chromosome 5.
DR   Proteomes; UP000007752; Chromosome 5.
DR   Proteomes; UP000059680; Chromosome 5.
DR   Genevisible; Q60DX1; OS.
DR   GO; GO:0005829; C:cytosol; IEA:UniProtKB-SubCell.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR   GO; GO:0016301; F:kinase activity; IEA:UniProtKB-KW.
DR   GO; GO:0030975; F:thiamine binding; IEA:InterPro.
DR   GO; GO:0004788; F:thiamine diphosphokinase activity; IBA:GO_Central.
DR   GO; GO:0016310; P:phosphorylation; IEA:UniProtKB-KW.
DR   GO; GO:0009229; P:thiamine diphosphate biosynthetic process; IBA:GO_Central.
DR   GO; GO:0006772; P:thiamine metabolic process; IEA:InterPro.
DR   CDD; cd07995; TPK; 1.
DR   Gene3D; 3.40.50.10240; -; 1.
DR   InterPro; IPR006282; Thi_PPkinase.
DR   InterPro; IPR016966; Thiamin_pyrophosphokinase_euk.
DR   InterPro; IPR007373; Thiamin_PyroPKinase_B1-bd.
DR   InterPro; IPR036371; TPK_B1-bd_sf.
DR   InterPro; IPR007371; TPK_catalytic.
DR   InterPro; IPR036759; TPK_catalytic_sf.
DR   Pfam; PF04265; TPK_B1_binding; 1.
DR   Pfam; PF04263; TPK_catalytic; 1.
DR   PIRSF; PIRSF031057; Thiamin_pyrophosphokinase; 1.
DR   SMART; SM00983; TPK_B1_binding; 1.
DR   SUPFAM; SSF63862; SSF63862; 1.
DR   SUPFAM; SSF63999; SSF63999; 1.
DR   TIGRFAMs; TIGR01378; thi_PPkinase; 1.
PE   2: Evidence at transcript level;
KW   ATP-binding; Cytoplasm; Kinase; Nucleotide-binding; Reference proteome;
KW   Transferase.
FT   CHAIN           1..267
FT                   /note="Thiamine pyrophosphokinase 3"
FT                   /id="PRO_0000423970"
FT   CONFLICT        29
FT                   /note="G -> C (in Ref. 5; EEE63458)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   267 AA;  29253 MW;  3029E9B6A8F2CF38 CRC64;
     MAPRPAMSHS SAFLLPSPSA AAAGADADGA AYALLVLNQR LPRFAPRLWD RAQVRVCADG
     GANRVFDGMP ELFPGQDPDE VRRRYKPDVI KGDLDSVRPE VKEYYSNMGT QIVDESHDQD
     TTDLHKCVAF ITENSAIPNK SNLCIFALGA LGGRFDHEMG NINVLHLFPN NRIILLSDDC
     LIFLLPRTHT HNIHIERSIE GPHCGLIPIG APSATTTTTG LQWNLDNTSM SFGGLISTSN
     IVREESTVVT ITSDSDLIWT ISLRHHS