TPL_CLOTE
ID TPL_CLOTE Reviewed; 460 AA.
AC Q897C2;
DT 06-JUN-2003, integrated into UniProtKB/Swiss-Prot.
DT 06-JUN-2003, sequence version 1.
DT 03-AUG-2022, entry version 104.
DE RecName: Full=Tyrosine phenol-lyase {ECO:0000255|HAMAP-Rule:MF_00543};
DE EC=4.1.99.2 {ECO:0000255|HAMAP-Rule:MF_00543};
DE AltName: Full=Beta-tyrosinase {ECO:0000255|HAMAP-Rule:MF_00543};
GN Name=tpl {ECO:0000255|HAMAP-Rule:MF_00543}; OrderedLocusNames=CTC_00818;
OS Clostridium tetani (strain Massachusetts / E88).
OC Bacteria; Firmicutes; Clostridia; Eubacteriales; Clostridiaceae;
OC Clostridium.
OX NCBI_TaxID=212717;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Massachusetts / E88;
RX PubMed=12552129; DOI=10.1073/pnas.0335853100;
RA Brueggemann H., Baeumer S., Fricke W.F., Wiezer A., Liesegang H.,
RA Decker I., Herzberg C., Martinez-Arias R., Merkl R., Henne A.,
RA Gottschalk G.;
RT "The genome sequence of Clostridium tetani, the causative agent of tetanus
RT disease.";
RL Proc. Natl. Acad. Sci. U.S.A. 100:1316-1321(2003).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=H2O + L-tyrosine = NH4(+) + phenol + pyruvate;
CC Xref=Rhea:RHEA:21704, ChEBI:CHEBI:15361, ChEBI:CHEBI:15377,
CC ChEBI:CHEBI:15882, ChEBI:CHEBI:28938, ChEBI:CHEBI:58315; EC=4.1.99.2;
CC Evidence={ECO:0000255|HAMAP-Rule:MF_00543};
CC -!- COFACTOR:
CC Name=pyridoxal 5'-phosphate; Xref=ChEBI:CHEBI:597326;
CC Evidence={ECO:0000255|HAMAP-Rule:MF_00543};
CC -!- SUBUNIT: Homotetramer. {ECO:0000255|HAMAP-Rule:MF_00543}.
CC -!- SIMILARITY: Belongs to the beta-eliminating lyase family.
CC {ECO:0000255|HAMAP-Rule:MF_00543}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAO35416.1; Type=Erroneous initiation; Evidence={ECO:0000305};
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DR EMBL; AE015927; AAO35416.1; ALT_INIT; Genomic_DNA.
DR RefSeq; WP_035110530.1; NC_004557.1.
DR AlphaFoldDB; Q897C2; -.
DR SMR; Q897C2; -.
DR STRING; 212717.CTC_00818; -.
DR PRIDE; Q897C2; -.
DR EnsemblBacteria; AAO35416; AAO35416; CTC_00818.
DR GeneID; 64181064; -.
DR KEGG; ctc:CTC_00818; -.
DR HOGENOM; CLU_047223_0_0_9; -.
DR OMA; VYTYAHM; -.
DR OrthoDB; 91973at2; -.
DR Proteomes; UP000001412; Chromosome.
DR GO; GO:0050371; F:tyrosine phenol-lyase activity; IEA:UniProtKB-UniRule.
DR GO; GO:0006570; P:tyrosine metabolic process; IEA:InterPro.
DR Gene3D; 3.40.640.10; -; 1.
DR Gene3D; 3.90.1150.10; -; 1.
DR HAMAP; MF_00543; Tyr_phenol_lyase; 1.
DR InterPro; IPR001597; ArAA_b-elim_lyase/Thr_aldolase.
DR InterPro; IPR011166; Beta-eliminating_lyase.
DR InterPro; IPR015424; PyrdxlP-dep_Trfase.
DR InterPro; IPR015421; PyrdxlP-dep_Trfase_major.
DR InterPro; IPR015422; PyrdxlP-dep_Trfase_small.
DR InterPro; IPR018176; Tryptophanase_CS.
DR InterPro; IPR013441; Tyr_phenol_ly.
DR Pfam; PF01212; Beta_elim_lyase; 1.
DR PIRSF; PIRSF001386; Trpase; 1.
DR SUPFAM; SSF53383; SSF53383; 1.
DR TIGRFAMs; TIGR02618; tyr_phenol_ly; 1.
DR PROSITE; PS00853; BETA_ELIM_LYASE; 1.
PE 3: Inferred from homology;
KW Lyase; Pyridoxal phosphate; Reference proteome.
FT CHAIN 1..460
FT /note="Tyrosine phenol-lyase"
FT /id="PRO_0000195632"
FT MOD_RES 260
FT /note="N6-(pyridoxal phosphate)lysine"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00543"
SQ SEQUENCE 460 AA; 51760 MW; 57F19258AD7813C1 CRC64;
MDLSKYPAEP FKIKSVEPVK MISREEREIA MKEAGYNTFN LRSEDVYIDL LTDSGTNAMS
DAQWAGMMIG DEAYAGSKNW LFLESTIKEL FGFKHVVPTH QGRGAENLLS SIAIKPGQYV
AGNMYFTTTR YHQEKNGGIF VDIIRDEAHD ASIDIPFKGN IDVNKLEKLI EEKGAENIAY
VCLAVTVNLA GGQPVSMANM RAVRELTAKH GIKVFYDATR CVENAYFIKE QEEGYADVSI
KDIVHEMFSY SDGATMSGKK DGIVNIGGFL AINDEELFGK AKEIVVVYEG MPSYGGMTGR
DMQAIAIGFR EAMQYEYIEH RIRQVRYLGD RLKEAGVPIV EPVGGHAVFL DARRFCPHLD
QEQFPAQSLA ASLYIDSGVR SMERGIVSAG RDVNTGENHK PKLETVRLTI PRRVYTYKHM
DVVAESVIHL YKHKEDIRPL KFTYEPAQLR FFTAKFDYAD
