TPL_ENTAG
ID TPL_ENTAG Reviewed; 456 AA.
AC P31011;
DT 01-JUL-1993, integrated into UniProtKB/Swiss-Prot.
DT 01-FEB-1996, sequence version 2.
DT 25-MAY-2022, entry version 106.
DE RecName: Full=Tyrosine phenol-lyase;
DE EC=4.1.99.2;
DE AltName: Full=Beta-tyrosinase;
GN Name=tpl; Synonyms=tutA;
OS Enterobacter agglomerans (Erwinia herbicola) (Pantoea agglomerans).
OC Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC Erwiniaceae; Pantoea; Pantoea agglomerans group.
OX NCBI_TaxID=549;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=MT-10509;
RX PubMed=1418690;
RA Iwamori S., Oikawa T., Ishiwata K., Makiguchi N.;
RT "Cloning and expression of the Erwinia herbicola tyrosine phenol-lyase gene
RT in Escherichia coli.";
RL Biotechnol. Appl. Biochem. 16:77-85(1992).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND PROTEIN SEQUENCE OF 1-11.
RC STRAIN=AJ2982;
RA Suzuki H., Nishihara K., Usui N., Matsui H., Kumagai H.;
RT "Cloning and nucleotide sequence of Erwinia herbicola AJ2982 tyrosine
RT phenol-lyase gene.";
RL J. Ferment. Bioeng. 75:145-148(1993).
RN [3]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=ATCC 21434 / AJ 2985;
RA Foor F.;
RL Submitted (APR-1995) to the EMBL/GenBank/DDBJ databases.
RN [4]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-51.
RX PubMed=8215376; DOI=10.1128/aem.59.9.3070-3075.1993;
RA Foor F., Morin N., Bostian K.;
RT "Production of L-dihydroxyphenylalanine in Escherichia coli with the
RT tyrosine phenol-lyase gene cloned from Erwinia herbicola.";
RL Appl. Environ. Microbiol. 59:3070-3075(1993).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=H2O + L-tyrosine = NH4(+) + phenol + pyruvate;
CC Xref=Rhea:RHEA:21704, ChEBI:CHEBI:15361, ChEBI:CHEBI:15377,
CC ChEBI:CHEBI:15882, ChEBI:CHEBI:28938, ChEBI:CHEBI:58315; EC=4.1.99.2;
CC -!- COFACTOR:
CC Name=pyridoxal 5'-phosphate; Xref=ChEBI:CHEBI:597326;
CC -!- SUBUNIT: Homotetramer.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000305}.
CC -!- INDUCTION: By L-tyrosine.
CC -!- PTM: Contains L-DOPA (3',4'-dihydroxyphenylalanine).
CC -!- SIMILARITY: Belongs to the beta-eliminating lyase family.
CC {ECO:0000305}.
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DR EMBL; S50513; AAB24234.1; -; Genomic_DNA.
DR EMBL; D13714; BAA02867.1; -; Genomic_DNA.
DR EMBL; U25347; AAA66390.1; -; Genomic_DNA.
DR EMBL; L08484; AAA71928.1; -; Genomic_DNA.
DR PDB; 1C7G; X-ray; 2.10 A; A/B/C/D=1-456.
DR PDBsum; 1C7G; -.
DR AlphaFoldDB; P31011; -.
DR SMR; P31011; -.
DR PRIDE; P31011; -.
DR EvolutionaryTrace; P31011; -.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0050371; F:tyrosine phenol-lyase activity; IEA:UniProtKB-UniRule.
DR GO; GO:0006570; P:tyrosine metabolic process; IEA:InterPro.
DR Gene3D; 3.40.640.10; -; 1.
DR Gene3D; 3.90.1150.10; -; 1.
DR HAMAP; MF_00543; Tyr_phenol_lyase; 1.
DR InterPro; IPR001597; ArAA_b-elim_lyase/Thr_aldolase.
DR InterPro; IPR011166; Beta-eliminating_lyase.
DR InterPro; IPR015424; PyrdxlP-dep_Trfase.
DR InterPro; IPR015421; PyrdxlP-dep_Trfase_major.
DR InterPro; IPR015422; PyrdxlP-dep_Trfase_small.
DR InterPro; IPR018176; Tryptophanase_CS.
DR InterPro; IPR013441; Tyr_phenol_ly.
DR Pfam; PF01212; Beta_elim_lyase; 1.
DR PIRSF; PIRSF001386; Trpase; 1.
DR SUPFAM; SSF53383; SSF53383; 1.
DR TIGRFAMs; TIGR02618; tyr_phenol_ly; 1.
DR PROSITE; PS00853; BETA_ELIM_LYASE; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Cytoplasm; Direct protein sequencing; Lyase;
KW Pyridoxal phosphate.
FT CHAIN 1..456
FT /note="Tyrosine phenol-lyase"
FT /id="PRO_0000195633"
FT MOD_RES 257
FT /note="N6-(pyridoxal phosphate)lysine"
FT /evidence="ECO:0000250"
FT VARIANT 157
FT /note="D -> N (in strain: MT-10509)"
FT VARIANT 212
FT /note="F -> Y (in strain: MT-10509)"
FT VARIANT 397
FT /note="R -> S (in strain: MT-10509)"
FT VARIANT 420
FT /note="V -> I (in strain: MT-10509)"
FT STRAND 7..15
FT /evidence="ECO:0007829|PDB:1C7G"
FT HELIX 21..30
FT /evidence="ECO:0007829|PDB:1C7G"
FT TURN 31..33
FT /evidence="ECO:0007829|PDB:1C7G"
FT HELIX 35..37
FT /evidence="ECO:0007829|PDB:1C7G"
FT HELIX 40..42
FT /evidence="ECO:0007829|PDB:1C7G"
FT STRAND 44..46
FT /evidence="ECO:0007829|PDB:1C7G"
FT STRAND 50..52
FT /evidence="ECO:0007829|PDB:1C7G"
FT HELIX 58..63
FT /evidence="ECO:0007829|PDB:1C7G"
FT STRAND 71..73
FT /evidence="ECO:0007829|PDB:1C7G"
FT HELIX 75..88
FT /evidence="ECO:0007829|PDB:1C7G"
FT STRAND 91..98
FT /evidence="ECO:0007829|PDB:1C7G"
FT HELIX 99..110
FT /evidence="ECO:0007829|PDB:1C7G"
FT STRAND 115..121
FT /evidence="ECO:0007829|PDB:1C7G"
FT HELIX 124..132
FT /evidence="ECO:0007829|PDB:1C7G"
FT STRAND 135..139
FT /evidence="ECO:0007829|PDB:1C7G"
FT HELIX 143..146
FT /evidence="ECO:0007829|PDB:1C7G"
FT HELIX 160..170
FT /evidence="ECO:0007829|PDB:1C7G"
FT HELIX 172..174
FT /evidence="ECO:0007829|PDB:1C7G"
FT STRAND 175..184
FT /evidence="ECO:0007829|PDB:1C7G"
FT TURN 185..188
FT /evidence="ECO:0007829|PDB:1C7G"
FT HELIX 194..207
FT /evidence="ECO:0007829|PDB:1C7G"
FT STRAND 211..214
FT /evidence="ECO:0007829|PDB:1C7G"
FT HELIX 218..228
FT /evidence="ECO:0007829|PDB:1C7G"
FT HELIX 237..245
FT /evidence="ECO:0007829|PDB:1C7G"
FT STRAND 249..254
FT /evidence="ECO:0007829|PDB:1C7G"
FT TURN 255..259
FT /evidence="ECO:0007829|PDB:1C7G"
FT STRAND 265..270
FT /evidence="ECO:0007829|PDB:1C7G"
FT HELIX 272..285
FT /evidence="ECO:0007829|PDB:1C7G"
FT TURN 289..293
FT /evidence="ECO:0007829|PDB:1C7G"
FT HELIX 296..309
FT /evidence="ECO:0007829|PDB:1C7G"
FT HELIX 312..331
FT /evidence="ECO:0007829|PDB:1C7G"
FT STRAND 342..348
FT /evidence="ECO:0007829|PDB:1C7G"
FT HELIX 349..352
FT /evidence="ECO:0007829|PDB:1C7G"
FT HELIX 358..360
FT /evidence="ECO:0007829|PDB:1C7G"
FT HELIX 362..374
FT /evidence="ECO:0007829|PDB:1C7G"
FT STRAND 375..377
FT /evidence="ECO:0007829|PDB:1C7G"
FT STRAND 379..382
FT /evidence="ECO:0007829|PDB:1C7G"
FT HELIX 383..386
FT /evidence="ECO:0007829|PDB:1C7G"
FT STRAND 390..392
FT /evidence="ECO:0007829|PDB:1C7G"
FT STRAND 402..406
FT /evidence="ECO:0007829|PDB:1C7G"
FT HELIX 414..428
FT /evidence="ECO:0007829|PDB:1C7G"
FT HELIX 429..433
FT /evidence="ECO:0007829|PDB:1C7G"
FT STRAND 437..441
FT /evidence="ECO:0007829|PDB:1C7G"
FT STRAND 444..446
FT /evidence="ECO:0007829|PDB:1C7G"
FT HELIX 447..450
FT /evidence="ECO:0007829|PDB:1C7G"
FT STRAND 452..455
FT /evidence="ECO:0007829|PDB:1C7G"
SQ SEQUENCE 456 AA; 51365 MW; 34758B48AF7C4AE2 CRC64;
MNYPAEPFRI KSVETVSMIS RDERVKKMQE AGYNTFLLNS KDIYIDLLTD SGTNAMSDKQ
WAGMMIGDEA YAGSENFYHL EKTVKELFGF KHIVPTHQGR GAENLLSQLA IKPGQYVAGN
MYFTTTRFHQ EKNGATFVDI VRDEAHDASL NLPFKGDIDL NKLATLIKEK GAENIAYICL
AVTVNLAGGQ PVSMANMRAV HEMASTYGIK IFYDATRCVE NAYFIKEQEA GYENVSIKDI
VHEMFSYADG CTMSGKKDCL VNIGGFLCMN DEEMFSAAKE LVVVYEGMPS YGGLAGRDME
AMAIGLREAM QYEYIEHRVK QVRYLGDKLR EAGVPIVEPT GGHAVFLDAR RFCPHLTQDQ
FPAQSLAASI YMETGVRSME RGIVSAGRSK ETGENHRPKL ETVRLTIPRR VYTYAHMDVV
ADGIIKLYQH KEDIRGLTFV YEPKQLRFFT ARFDFI
