ID   TPL_INTC7               Reviewed;         473 AA.
AC   E6SFG5;
DT   31-MAY-2011, integrated into UniProtKB/Swiss-Prot.
DT   08-MAR-2011, sequence version 1.
DT   25-MAY-2022, entry version 46.
DE   RecName: Full=Tyrosine phenol-lyase {ECO:0000255|HAMAP-Rule:MF_00543};
DE            EC=4.1.99.2 {ECO:0000255|HAMAP-Rule:MF_00543};
DE   AltName: Full=Beta-tyrosinase {ECO:0000255|HAMAP-Rule:MF_00543};
GN   Name=tpl {ECO:0000255|HAMAP-Rule:MF_00543}; OrderedLocusNames=Intca_0142;
OS   Intrasporangium calvum (strain ATCC 23552 / DSM 43043 / JCM 3097 / NBRC
OS   12989 / 7 KIP).
OC   Bacteria; Actinobacteria; Micrococcales; Intrasporangiaceae;
OC   Intrasporangium.
OX   NCBI_TaxID=710696;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 23552 / DSM 43043 / JCM 3097 / NBRC 12989 / 7 KIP;
RX   PubMed=21304734; DOI=10.4056/sigs.1263355;
RA   Del Rio T.G., Chertkov O., Yasawong M., Lucas S., Deshpande S., Cheng J.F.,
RA   Detter C., Tapia R., Han C., Goodwin L., Pitluck S., Liolios K.,
RA   Ivanova N., Mavromatis K., Pati A., Chen A., Palaniappan K., Land M.,
RA   Hauser L., Chang Y.J., Jeffries C.D., Rohde M., Pukall R., Sikorski J.,
RA   Goker M., Woyke T., Bristow J., Eisen J.A., Markowitz V., Hugenholtz P.,
RA   Kyrpides N.C., Klenk H.P., Lapidus A.;
RT   "Complete genome sequence of Intrasporangium calvum type strain (7 KIP).";
RL   Stand. Genomic Sci. 3:294-303(2010).
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=H2O + L-tyrosine = NH4(+) + phenol + pyruvate;
CC         Xref=Rhea:RHEA:21704, ChEBI:CHEBI:15361, ChEBI:CHEBI:15377,
CC         ChEBI:CHEBI:15882, ChEBI:CHEBI:28938, ChEBI:CHEBI:58315; EC=4.1.99.2;
CC         Evidence={ECO:0000255|HAMAP-Rule:MF_00543};
CC   -!- COFACTOR:
CC       Name=pyridoxal 5'-phosphate; Xref=ChEBI:CHEBI:597326;
CC         Evidence={ECO:0000255|HAMAP-Rule:MF_00543};
CC   -!- SUBUNIT: Homotetramer. {ECO:0000255|HAMAP-Rule:MF_00543}.
CC   -!- SIMILARITY: Belongs to the beta-eliminating lyase family.
CC       {ECO:0000255|HAMAP-Rule:MF_00543}.
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DR   EMBL; CP002343; ADU46703.1; -; Genomic_DNA.
DR   RefSeq; WP_013491025.1; NC_014830.1.
DR   AlphaFoldDB; E6SFG5; -.
DR   SMR; E6SFG5; -.
DR   STRING; 710696.Intca_0142; -.
DR   EnsemblBacteria; ADU46703; ADU46703; Intca_0142.
DR   KEGG; ica:Intca_0142; -.
DR   eggNOG; COG3033; Bacteria.
DR   HOGENOM; CLU_047223_0_0_11; -.
DR   OMA; VYTYAHM; -.
DR   OrthoDB; 91973at2; -.
DR   Proteomes; UP000008914; Chromosome.
DR   GO; GO:0050371; F:tyrosine phenol-lyase activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0006570; P:tyrosine metabolic process; IEA:InterPro.
DR   Gene3D; 3.40.640.10; -; 1.
DR   Gene3D; 3.90.1150.10; -; 1.
DR   HAMAP; MF_00543; Tyr_phenol_lyase; 1.
DR   InterPro; IPR001597; ArAA_b-elim_lyase/Thr_aldolase.
DR   InterPro; IPR011166; Beta-eliminating_lyase.
DR   InterPro; IPR015424; PyrdxlP-dep_Trfase.
DR   InterPro; IPR015421; PyrdxlP-dep_Trfase_major.
DR   InterPro; IPR015422; PyrdxlP-dep_Trfase_small.
DR   InterPro; IPR013441; Tyr_phenol_ly.
DR   Pfam; PF01212; Beta_elim_lyase; 1.
DR   PIRSF; PIRSF001386; Trpase; 1.
DR   SUPFAM; SSF53383; SSF53383; 1.
DR   TIGRFAMs; TIGR02618; tyr_phenol_ly; 1.
PE   3: Inferred from homology;
KW   Lyase; Pyridoxal phosphate; Reference proteome.
FT   CHAIN           1..473
FT                   /note="Tyrosine phenol-lyase"
FT                   /id="PRO_0000408493"
FT   MOD_RES         257
FT                   /note="N6-(pyridoxal phosphate)lysine"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00543"
SQ   SEQUENCE   473 AA;  52319 MW;  5539710C01C49799 CRC64;
     MPAIAEPYRI KMVEPITMTT REQREAAIRA AGYNTFLLRS DDVYIDLLTD SGTSAMSDRQ
     WSAMMMGDEA YAGARSFDHL VESVRENYGF PLVCPTHQGR GAEHLISKIL ITPGQHVPGN
     MYFTTTRVHQ ELAGGTFHDV IIDEAHDPAS RHPFKGNVDL DKFQALIDEV GADQIAYINV
     AVTVNMAGGQ PVSMANIRAV REICDRYGII LWSDATRLAE NAWFIQERED GYAGRPVAEI
     VREMLSHFDG ITMSGKKDCL VNIGGFLAMR DEAILTKARE LVVVYEGMPT YGGLAGRDLD
     AMAVGLREAL DDDYLTHRIG QVRYLGERLQ AAGVPIVEPI GGHAVFIDAA AFLDHLSQDE
     LPAQTLAAEI FLDSGVRSME RGIVSAGRDA DGNNRYPKLE LVRLTIPRRV YTNSHMDVVA
     DSVIRVFQRR REIQGLTFVY ESPTLRFFTS RFEPLAAEAA LEEGRPEPAL AGA