ID   TPL_NOSP7               Reviewed;         467 AA.
AC   B2J908;
DT   31-MAY-2011, integrated into UniProtKB/Swiss-Prot.
DT   10-JUN-2008, sequence version 1.
DT   25-MAY-2022, entry version 68.
DE   RecName: Full=Tyrosine phenol-lyase {ECO:0000255|HAMAP-Rule:MF_00543};
DE            EC=4.1.99.2 {ECO:0000255|HAMAP-Rule:MF_00543};
DE   AltName: Full=Beta-tyrosinase {ECO:0000255|HAMAP-Rule:MF_00543};
GN   Name=tpl {ECO:0000255|HAMAP-Rule:MF_00543}; OrderedLocusNames=Npun_R5724;
OS   Nostoc punctiforme (strain ATCC 29133 / PCC 73102).
OC   Bacteria; Cyanobacteria; Nostocales; Nostocaceae; Nostoc.
OX   NCBI_TaxID=63737;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 29133 / PCC 73102;
RG   US DOE Joint Genome Institute;
RA   Copeland A., Lucas S., Lapidus A., Glavina del Rio T., Dalin E., Tice H.,
RA   Pitluck S., Chain P., Malfatti S., Shin M., Vergez L., Schmutz J.,
RA   Larimer F., Land M., Hauser L., Kyrpides N., Kim E., Meeks J.C., Elhai J.,
RA   Campbell E.L., Thiel T., Longmire J., Potts M., Atlas R.;
RT   "Complete sequence of chromosome of Nostoc punctiforme ATCC 29133.";
RL   Submitted (APR-2008) to the EMBL/GenBank/DDBJ databases.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=H2O + L-tyrosine = NH4(+) + phenol + pyruvate;
CC         Xref=Rhea:RHEA:21704, ChEBI:CHEBI:15361, ChEBI:CHEBI:15377,
CC         ChEBI:CHEBI:15882, ChEBI:CHEBI:28938, ChEBI:CHEBI:58315; EC=4.1.99.2;
CC         Evidence={ECO:0000255|HAMAP-Rule:MF_00543};
CC   -!- COFACTOR:
CC       Name=pyridoxal 5'-phosphate; Xref=ChEBI:CHEBI:597326;
CC         Evidence={ECO:0000255|HAMAP-Rule:MF_00543};
CC   -!- SUBUNIT: Homotetramer. {ECO:0000255|HAMAP-Rule:MF_00543}.
CC   -!- SIMILARITY: Belongs to the beta-eliminating lyase family.
CC       {ECO:0000255|HAMAP-Rule:MF_00543}.
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DR   EMBL; CP001037; ACC84024.1; -; Genomic_DNA.
DR   RefSeq; WP_012411967.1; NC_010628.1.
DR   AlphaFoldDB; B2J908; -.
DR   SMR; B2J908; -.
DR   STRING; 63737.Npun_R5724; -.
DR   EnsemblBacteria; ACC84024; ACC84024; Npun_R5724.
DR   KEGG; npu:Npun_R5724; -.
DR   eggNOG; COG3033; Bacteria.
DR   HOGENOM; CLU_047223_0_0_3; -.
DR   OMA; EMYQYGD; -.
DR   OrthoDB; 91973at2; -.
DR   Proteomes; UP000001191; Chromosome.
DR   GO; GO:0050371; F:tyrosine phenol-lyase activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0006570; P:tyrosine metabolic process; IEA:InterPro.
DR   Gene3D; 3.40.640.10; -; 1.
DR   Gene3D; 3.90.1150.10; -; 1.
DR   HAMAP; MF_00543; Tyr_phenol_lyase; 1.
DR   InterPro; IPR001597; ArAA_b-elim_lyase/Thr_aldolase.
DR   InterPro; IPR011166; Beta-eliminating_lyase.
DR   InterPro; IPR015424; PyrdxlP-dep_Trfase.
DR   InterPro; IPR015421; PyrdxlP-dep_Trfase_major.
DR   InterPro; IPR015422; PyrdxlP-dep_Trfase_small.
DR   InterPro; IPR018176; Tryptophanase_CS.
DR   InterPro; IPR013441; Tyr_phenol_ly.
DR   Pfam; PF01212; Beta_elim_lyase; 1.
DR   PIRSF; PIRSF001386; Trpase; 1.
DR   SUPFAM; SSF53383; SSF53383; 1.
DR   TIGRFAMs; TIGR02618; tyr_phenol_ly; 1.
DR   PROSITE; PS00853; BETA_ELIM_LYASE; 1.
PE   3: Inferred from homology;
KW   Lyase; Pyridoxal phosphate; Reference proteome.
FT   CHAIN           1..467
FT                   /note="Tyrosine phenol-lyase"
FT                   /id="PRO_0000408494"
FT   MOD_RES         268
FT                   /note="N6-(pyridoxal phosphate)lysine"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00543"
SQ   SEQUENCE   467 AA;  52890 MW;  2B9F33F7BCE9480E CRC64;
     MTDAKQTSPR RRRSWAEPYK IKVVEPLKIT TRAEREQAIA QAGYNTFLLR SEDVYIDLLT
     DSGTSAMSDY QWAGMMLGDE AYAGSKNFYN LEASIQKYYG YRHIVPTHQG RGAENILSQI
     LIKPGDYIPG NMYFTTTRLH QELAGGTFVD VIIDEAHDAQ SLHPFKGNVD LQKLTDLIER
     VGAERIPYIS VAGTVNMAGG QPISMANLRA VHQLAQTYGI RIILDATRAV ENAHFIQQRE
     EDYSSQAIAT ILREFCSYTD GCTMSGKKDA LVNIGGWLAL NDYNLYEEAR NLIVIYEGLH
     TYGGMAGRDM EAMARGIEES VQDDHIRARV GQVEYLGQKL LDWGIPIVVP IGGHAIYLDA
     KRFLPQIPQD QFPAQRLAAE LYLEAGIRAM ERGIVSAGRN KETGDNYYPE LELVRLTIPR
     RVYTQAHMDL TAEAVEEVYH NRDRLRGLKM IYEPKYLRFF QARFELQ