ID   TPL_SYMS1               Reviewed;         458 AA.
AC   O08501;
DT   27-MAR-2002, integrated into UniProtKB/Swiss-Prot.
DT   01-JUL-1997, sequence version 1.
DT   25-MAY-2022, entry version 85.
DE   RecName: Full=Tyrosine phenol-lyase;
DE            EC=4.1.99.2;
DE   AltName: Full=Beta-tyrosinase;
GN   Name=tpl;
OS   Symbiobacterium sp. (strain SC-1).
OC   Bacteria; Firmicutes; Clostridia; Eubacteriales; Symbiobacteriaceae;
OC   Symbiobacterium; unclassified Symbiobacterium.
OX   NCBI_TaxID=56197;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RA   Sung M.H., Hong S.P., Lee S.G.;
RT   "Thermostable tyrosine phenol-lyase of Symbiobacterium sp. SC-1; cloning,
RT   overproduction, and enzyme properties.";
RL   Submitted (JAN-1997) to the EMBL/GenBank/DDBJ databases.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=H2O + L-tyrosine = NH4(+) + phenol + pyruvate;
CC         Xref=Rhea:RHEA:21704, ChEBI:CHEBI:15361, ChEBI:CHEBI:15377,
CC         ChEBI:CHEBI:15882, ChEBI:CHEBI:28938, ChEBI:CHEBI:58315; EC=4.1.99.2;
CC   -!- COFACTOR:
CC       Name=pyridoxal 5'-phosphate; Xref=ChEBI:CHEBI:597326;
CC         Evidence={ECO:0000250};
CC   -!- SUBUNIT: Homotetramer. {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the beta-eliminating lyase family.
CC       {ECO:0000305}.
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DR   EMBL; U83917; AAB41499.1; -; Genomic_DNA.
DR   AlphaFoldDB; O08501; -.
DR   SMR; O08501; -.
DR   GO; GO:0050371; F:tyrosine phenol-lyase activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0006570; P:tyrosine metabolic process; IEA:InterPro.
DR   Gene3D; 3.40.640.10; -; 1.
DR   Gene3D; 3.90.1150.10; -; 1.
DR   HAMAP; MF_00543; Tyr_phenol_lyase; 1.
DR   InterPro; IPR001597; ArAA_b-elim_lyase/Thr_aldolase.
DR   InterPro; IPR011166; Beta-eliminating_lyase.
DR   InterPro; IPR015424; PyrdxlP-dep_Trfase.
DR   InterPro; IPR015421; PyrdxlP-dep_Trfase_major.
DR   InterPro; IPR015422; PyrdxlP-dep_Trfase_small.
DR   InterPro; IPR018176; Tryptophanase_CS.
DR   InterPro; IPR013441; Tyr_phenol_ly.
DR   Pfam; PF01212; Beta_elim_lyase; 1.
DR   PIRSF; PIRSF001386; Trpase; 1.
DR   SUPFAM; SSF53383; SSF53383; 1.
DR   TIGRFAMs; TIGR02618; tyr_phenol_ly; 1.
DR   PROSITE; PS00853; BETA_ELIM_LYASE; 1.
PE   3: Inferred from homology;
KW   Lyase; Pyridoxal phosphate.
FT   CHAIN           1..458
FT                   /note="Tyrosine phenol-lyase"
FT                   /id="PRO_0000195637"
FT   MOD_RES         258
FT                   /note="N6-(pyridoxal phosphate)lysine"
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   458 AA;  52190 MW;  D7F1EAB6E79851D9 CRC64;
     MQRPWAEPYK IKAVEPIRMT TREYREQAIR EAGYNTFLLR SEDVYIDLLT DSGTNAMSDR
     QWGALMMGDE AYAGARSFFR LEEAVREIYG FKYVVPTHQG RGAEHLISRI LIKPGDYIPG
     NMYFTTTRTH QELQGGTFVD VIIDEAHDPQ ANHPFKGNVD IAKFEALIDR VGADKIPYIN
     VALTVNMAGG QPVSMANLRE VRKVCDRHGI RMWSDATRAV ENAYFIKERE EGYQDKPVRE
     ILKEMMSYFD GCTMSGKKDC LVNIGGFLAM NEEWILQKAR EQVVIFEGMP TYGGLAGRDM
     EAIAQGIYEM VDDDYIAHRI HQVRYLGEQL LEAGIPIVQP IGGHAVFLDA RAFLPHIPQD
     QFPAQALAAA LYVDSGVRAM ERGIVSAGRN PQTGEHNYPK LELVRLTIPR RVYTDRHMDV
     VAYSVKHLWK ERDTIRGLRM VYEPPTLRFF TARFEPIS