TPM1_YEAST
ID TPM1_YEAST Reviewed; 199 AA.
AC P17536; D6W1A0; Q45TY1;
DT 01-AUG-1990, integrated into UniProtKB/Swiss-Prot.
DT 01-AUG-1990, sequence version 1.
DT 03-AUG-2022, entry version 167.
DE RecName: Full=Tropomyosin-1;
GN Name=TPM1; OrderedLocusNames=YNL079C; ORFNames=N2332;
OS Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC Saccharomycetales; Saccharomycetaceae; Saccharomyces.
OX NCBI_TaxID=559292;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX PubMed=2649250; DOI=10.1016/0092-8674(89)90961-6;
RA Liu H., Bretscher A.;
RT "Disruption of the single tropomyosin gene in yeast results in the
RT disappearance of actin cables from the cytoskeleton.";
RL Cell 57:233-242(1989).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=SK1;
RX PubMed=16273108; DOI=10.1038/ng1674;
RA Deutschbauer A.M., Davis R.W.;
RT "Quantitative trait loci mapped to single-nucleotide resolution in yeast.";
RL Nat. Genet. 37:1333-1340(2005).
RN [3]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=ATCC 96604 / S288c / FY1679;
RX PubMed=8701611;
RX DOI=10.1002/(sici)1097-0061(19960330)12:4<391::aid-yea921>3.0.co;2-n;
RA Poehlmann R., Philippsen P.;
RT "Sequencing a cosmid clone of Saccharomyces cerevisiae chromosome XIV
RT reveals 12 new open reading frames (ORFs) and an ancient duplication of six
RT ORFs.";
RL Yeast 12:391-402(1996).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=9169873;
RA Philippsen P., Kleine K., Poehlmann R., Duesterhoeft A., Hamberg K.,
RA Hegemann J.H., Obermaier B., Urrestarazu L.A., Aert R., Albermann K.,
RA Altmann R., Andre B., Baladron V., Ballesta J.P.G., Becam A.-M.,
RA Beinhauer J.D., Boskovic J., Buitrago M.J., Bussereau F., Coster F.,
RA Crouzet M., D'Angelo M., Dal Pero F., De Antoni A., del Rey F., Doignon F.,
RA Domdey H., Dubois E., Fiedler T.A., Fleig U., Floeth M., Fritz C.,
RA Gaillardin C., Garcia-Cantalejo J.M., Glansdorff N., Goffeau A.,
RA Gueldener U., Herbert C.J., Heumann K., Heuss-Neitzel D., Hilbert H.,
RA Hinni K., Iraqui Houssaini I., Jacquet M., Jimenez A., Jonniaux J.-L.,
RA Karpfinger-Hartl L., Lanfranchi G., Lepingle A., Levesque H., Lyck R.,
RA Maftahi M., Mallet L., Maurer C.T.C., Messenguy F., Mewes H.-W., Moestl D.,
RA Nasr F., Nicaud J.-M., Niedenthal R.K., Pandolfo D., Pierard A.,
RA Piravandi E., Planta R.J., Pohl T.M., Purnelle B., Rebischung C.,
RA Remacha M.A., Revuelta J.L., Rinke M., Saiz J.E., Sartorello F.,
RA Scherens B., Sen-Gupta M., Soler-Mira A., Urbanus J.H.M., Valle G.,
RA Van Dyck L., Verhasselt P., Vierendeels F., Vissers S., Voet M.,
RA Volckaert G., Wach A., Wambutt R., Wedler H., Zollner A., Hani J.;
RT "The nucleotide sequence of Saccharomyces cerevisiae chromosome XIV and its
RT evolutionary implications.";
RL Nature 387:93-98(1997).
RN [5]
RP GENOME REANNOTATION.
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=24374639; DOI=10.1534/g3.113.008995;
RA Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R.,
RA Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S.,
RA Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.;
RT "The reference genome sequence of Saccharomyces cerevisiae: Then and now.";
RL G3 (Bethesda) 4:389-398(2014).
RN [6]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=17322287; DOI=10.1101/gr.6037607;
RA Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F.,
RA Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J.,
RA Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J.,
RA Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G., Kolodner R.D.,
RA LaBaer J.;
RT "Approaching a complete repository of sequence-verified protein-encoding
RT clones for Saccharomyces cerevisiae.";
RL Genome Res. 17:536-543(2007).
RN [7]
RP LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
RX PubMed=14562106; DOI=10.1038/nature02046;
RA Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N.,
RA O'Shea E.K., Weissman J.S.;
RT "Global analysis of protein expression in yeast.";
RL Nature 425:737-741(2003).
RN [8]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-195, AND IDENTIFICATION BY
RP MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX PubMed=18407956; DOI=10.1074/mcp.m700468-mcp200;
RA Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.;
RT "A multidimensional chromatography technology for in-depth phosphoproteome
RT analysis.";
RL Mol. Cell. Proteomics 7:1389-1396(2008).
RN [9]
RP UBIQUITINATION [LARGE SCALE ANALYSIS] AT LYS-39; LYS-59 AND LYS-187, AND
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX PubMed=22106047; DOI=10.1002/pmic.201100166;
RA Starita L.M., Lo R.S., Eng J.K., von Haller P.D., Fields S.;
RT "Sites of ubiquitin attachment in Saccharomyces cerevisiae.";
RL Proteomics 12:236-240(2012).
CC -!- SUBUNIT: Homodimer. {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm, cytoskeleton {ECO:0000305}.
CC -!- DOMAIN: The molecule is in a coiled coil structure that is formed by 2
CC polypeptide chains. The sequence exhibits a prominent seven-residues
CC periodicity.
CC -!- MISCELLANEOUS: Present with 3060 molecules/cell in log phase SD medium.
CC {ECO:0000269|PubMed:14562106}.
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DR EMBL; M25501; AAA35174.1; -; Genomic_DNA.
DR EMBL; DQ115393; AAZ22529.1; -; Genomic_DNA.
DR EMBL; X86470; CAA60179.1; -; Genomic_DNA.
DR EMBL; Z71355; CAA95953.1; -; Genomic_DNA.
DR EMBL; AY692838; AAT92857.1; -; Genomic_DNA.
DR EMBL; BK006947; DAA10466.1; -; Genomic_DNA.
DR PIR; A32183; A32183.
DR RefSeq; NP_014320.1; NM_001182917.1.
DR AlphaFoldDB; P17536; -.
DR SMR; P17536; -.
DR BioGRID; 35744; 690.
DR DIP; DIP-2992N; -.
DR IntAct; P17536; 3.
DR MINT; P17536; -.
DR STRING; 4932.YNL079C; -.
DR iPTMnet; P17536; -.
DR MaxQB; P17536; -.
DR PaxDb; P17536; -.
DR PRIDE; P17536; -.
DR EnsemblFungi; YNL079C_mRNA; YNL079C; YNL079C.
DR GeneID; 855645; -.
DR KEGG; sce:YNL079C; -.
DR SGD; S000005023; TPM1.
DR VEuPathDB; FungiDB:YNL079C; -.
DR eggNOG; KOG1003; Eukaryota.
DR GeneTree; ENSGT00940000176442; -.
DR HOGENOM; CLU_104738_1_0_1; -.
DR InParanoid; P17536; -.
DR OMA; AKYEEMA; -.
DR BioCyc; YEAST:G3O-33108-MON; -.
DR Reactome; R-SCE-9013424; RHOV GTPase cycle.
DR PRO; PR:P17536; -.
DR Proteomes; UP000002311; Chromosome XIV.
DR RNAct; P17536; protein.
DR GO; GO:0005884; C:actin filament; IBA:GO_Central.
DR GO; GO:0032432; C:actin filament bundle; IDA:SGD.
DR GO; GO:0005826; C:actomyosin contractile ring; IBA:GO_Central.
DR GO; GO:0000142; C:cellular bud neck contractile ring; IDA:SGD.
DR GO; GO:0051015; F:actin filament binding; IBA:GO_Central.
DR GO; GO:0003786; F:actin lateral binding; TAS:SGD.
DR GO; GO:0051017; P:actin filament bundle assembly; IGI:SGD.
DR GO; GO:0007015; P:actin filament organization; IMP:SGD.
DR GO; GO:0090527; P:actin filament reorganization; IMP:SGD.
DR GO; GO:0007118; P:budding cell apical bud growth; TAS:SGD.
DR GO; GO:0007119; P:budding cell isotropic bud growth; TAS:SGD.
DR GO; GO:0030010; P:establishment of cell polarity; TAS:SGD.
DR GO; GO:0006887; P:exocytosis; TAS:SGD.
DR GO; GO:0030447; P:filamentous growth; IMP:SGD.
DR GO; GO:0008298; P:intracellular mRNA localization; TAS:SGD.
DR GO; GO:0000001; P:mitochondrion inheritance; TAS:SGD.
DR GO; GO:1903475; P:mitotic actomyosin contractile ring assembly; IGI:SGD.
DR GO; GO:0000281; P:mitotic cytokinesis; IBA:GO_Central.
DR GO; GO:0007124; P:pseudohyphal growth; IMP:SGD.
DR GO; GO:0000011; P:vacuole inheritance; TAS:SGD.
DR GO; GO:0016192; P:vesicle-mediated transport; TAS:SGD.
DR InterPro; IPR000533; Tropomyosin.
DR Pfam; PF12718; Tropomyosin_1; 1.
PE 1: Evidence at protein level;
KW Coiled coil; Cytoplasm; Cytoskeleton; Isopeptide bond; Phosphoprotein;
KW Reference proteome; Repeat; Ubl conjugation.
FT CHAIN 1..199
FT /note="Tropomyosin-1"
FT /id="PRO_0000205692"
FT REGION 59..81
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 102..147
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COILED 1..199
FT /evidence="ECO:0000250"
FT COMPBIAS 125..147
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 195
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:18407956"
FT CROSSLNK 39
FT /note="Glycyl lysine isopeptide (Lys-Gly) (interchain with
FT G-Cter in ubiquitin)"
FT /evidence="ECO:0007744|PubMed:22106047"
FT CROSSLNK 59
FT /note="Glycyl lysine isopeptide (Lys-Gly) (interchain with
FT G-Cter in ubiquitin)"
FT /evidence="ECO:0007744|PubMed:22106047"
FT CROSSLNK 187
FT /note="Glycyl lysine isopeptide (Lys-Gly) (interchain with
FT G-Cter in ubiquitin)"
FT /evidence="ECO:0007744|PubMed:22106047"
SQ SEQUENCE 199 AA; 23541 MW; 845F3DB4387083BF CRC64;
MDKIREKLSN LKLEAESWQE KYEELKEKNK DLEQENVEKE NQIKSLTVKN QQLEDEIEKL
EAGLSDSKQT EQDNVEKENQ IKSLTVKNHQ LEEEIEKLEA ELAESKQLSE DSHHLQSNND
NFSKKNQQLE EDLEESDTKL KETTEKLRES DLKADQLERR VAALEEQREE WERKNEELTV
KYEDAKKELD EIAASLENL
