BTUD_ECOLI
ID BTUD_ECOLI Reviewed; 249 AA.
AC P06611;
DT 01-JAN-1988, integrated into UniProtKB/Swiss-Prot.
DT 01-JAN-1988, sequence version 1.
DT 03-AUG-2022, entry version 185.
DE RecName: Full=Vitamin B12 import ATP-binding protein BtuD {ECO:0000255|HAMAP-Rule:MF_01005};
DE EC=7.6.2.8 {ECO:0000255|HAMAP-Rule:MF_01005};
DE AltName: Full=Vitamin B12-transporting ATPase {ECO:0000255|HAMAP-Rule:MF_01005};
GN Name=btuD {ECO:0000255|HAMAP-Rule:MF_01005};
GN OrderedLocusNames=b1709, JW1699;
OS Escherichia coli (strain K12).
OC Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC Enterobacteriaceae; Escherichia.
OX NCBI_TaxID=83333;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX PubMed=3528129; DOI=10.1128/jb.167.3.928-934.1986;
RA Friedrich M.J., Deveaux L.C., Kadner R.J.;
RT "Nucleotide sequence of the btuCED genes involved in vitamin B12 transport
RT in Escherichia coli and homology with components of periplasmic-binding-
RT protein-dependent transport systems.";
RL J. Bacteriol. 167:928-934(1986).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RX PubMed=9097039; DOI=10.1093/dnares/3.6.363;
RA Aiba H., Baba T., Fujita K., Hayashi K., Inada T., Isono K., Itoh T.,
RA Kasai H., Kashimoto K., Kimura S., Kitakawa M., Kitagawa M., Makino K.,
RA Miki T., Mizobuchi K., Mori H., Mori T., Motomura K., Nakade S.,
RA Nakamura Y., Nashimoto H., Nishio Y., Oshima T., Saito N., Sampei G.,
RA Seki Y., Sivasundaram S., Tagami H., Takeda J., Takemoto K., Takeuchi Y.,
RA Wada C., Yamamoto Y., Horiuchi T.;
RT "A 570-kb DNA sequence of the Escherichia coli K-12 genome corresponding to
RT the 28.0-40.1 min region on the linkage map.";
RL DNA Res. 3:363-377(1996).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / MG1655 / ATCC 47076;
RX PubMed=9278503; DOI=10.1126/science.277.5331.1453;
RA Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V.,
RA Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F.,
RA Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B.,
RA Shao Y.;
RT "The complete genome sequence of Escherichia coli K-12.";
RL Science 277:1453-1462(1997).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RX PubMed=16738553; DOI=10.1038/msb4100049;
RA Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S.,
RA Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.;
RT "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655
RT and W3110.";
RL Mol. Syst. Biol. 2:E1-E5(2006).
RN [5]
RP X-RAY CRYSTALLOGRAPHY (3.2 ANGSTROMS) IN COMPLEX WITH BTUC.
RX PubMed=12004122; DOI=10.1126/science.1071142;
RA Locher K.P., Lee A.T., Rees D.C.;
RT "The E. coli BtuCD structure: a framework for ABC transporter architecture
RT and mechanism.";
RL Science 296:1091-1098(2002).
CC -!- FUNCTION: Part of the ABC transporter complex BtuCDF involved in
CC vitamin B12 import. Responsible for energy coupling to the transport
CC system. {ECO:0000255|HAMAP-Rule:MF_01005}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=an R-cob(III)alamin(out) + ATP + H2O = ADP + an R-
CC cob(III)alamin(in) + H(+) + phosphate; Xref=Rhea:RHEA:17873,
CC ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:30616,
CC ChEBI:CHEBI:43474, ChEBI:CHEBI:140785, ChEBI:CHEBI:456216;
CC EC=7.6.2.8; Evidence={ECO:0000255|HAMAP-Rule:MF_01005};
CC -!- SUBUNIT: The complex is composed of two ATP-binding proteins (BtuD),
CC two transmembrane proteins (BtuC) and a solute-binding protein (BtuF).
CC {ECO:0000255|HAMAP-Rule:MF_01005, ECO:0000269|PubMed:12004122}.
CC -!- INTERACTION:
CC P06611; P06609: btuC; NbExp=13; IntAct=EBI-1033420, EBI-1033427;
CC -!- SUBCELLULAR LOCATION: Cell inner membrane; Peripheral membrane protein.
CC -!- SIMILARITY: Belongs to the ABC transporter superfamily. Vitamin B12
CC importer (TC 3.A.1.13.1) family. {ECO:0000255|HAMAP-Rule:MF_01005}.
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DR EMBL; M14031; AAA23528.1; -; Genomic_DNA.
DR EMBL; U00096; AAC74779.1; -; Genomic_DNA.
DR EMBL; AP009048; BAA15477.1; -; Genomic_DNA.
DR PIR; C24498; QRECBD.
DR RefSeq; NP_416224.1; NC_000913.3.
DR RefSeq; WP_000029474.1; NZ_LN832404.1.
DR PDB; 1L7V; X-ray; 3.20 A; C/D=1-249.
DR PDB; 2QI9; X-ray; 2.60 A; C/D=1-249.
DR PDB; 4DBL; X-ray; 3.49 A; C/D/H/I=1-249.
DR PDB; 4FI3; X-ray; 3.47 A; C/D=1-249.
DR PDB; 4R9U; X-ray; 2.78 A; C/D=1-249.
DR PDBsum; 1L7V; -.
DR PDBsum; 2QI9; -.
DR PDBsum; 4DBL; -.
DR PDBsum; 4FI3; -.
DR PDBsum; 4R9U; -.
DR AlphaFoldDB; P06611; -.
DR SMR; P06611; -.
DR BioGRID; 4260301; 185.
DR ComplexPortal; CPX-2105; Cobalamin ABC transporter complex.
DR ComplexPortal; CPX-2106; BtuCD complex.
DR DIP; DIP-9234N; -.
DR IntAct; P06611; 9.
DR MINT; P06611; -.
DR STRING; 511145.b1709; -.
DR TCDB; 3.A.1.13.1; the atp-binding cassette (abc) superfamily.
DR jPOST; P06611; -.
DR PaxDb; P06611; -.
DR PRIDE; P06611; -.
DR EnsemblBacteria; AAC74779; AAC74779; b1709.
DR EnsemblBacteria; BAA15477; BAA15477; BAA15477.
DR GeneID; 945751; -.
DR KEGG; ecj:JW1699; -.
DR KEGG; eco:b1709; -.
DR PATRIC; fig|1411691.4.peg.548; -.
DR EchoBASE; EB0126; -.
DR eggNOG; COG4138; Bacteria.
DR HOGENOM; CLU_000604_1_11_6; -.
DR InParanoid; P06611; -.
DR OMA; HHADRVW; -.
DR PhylomeDB; P06611; -.
DR BioCyc; EcoCyc:BTUD-MON; -.
DR BioCyc; MetaCyc:BTUD-MON; -.
DR BRENDA; 7.6.2.8; 2026.
DR EvolutionaryTrace; P06611; -.
DR PRO; PR:P06611; -.
DR Proteomes; UP000000318; Chromosome.
DR Proteomes; UP000000625; Chromosome.
DR GO; GO:0043190; C:ATP-binding cassette (ABC) transporter complex; IDA:EcoCyc.
DR GO; GO:1990191; C:cobalamin transport complex; IPI:ComplexPortal.
DR GO; GO:0019898; C:extrinsic component of membrane; IDA:EcoliWiki.
DR GO; GO:0016020; C:membrane; IDA:ComplexPortal.
DR GO; GO:0015420; F:ABC-type vitamin B12 transporter activity; IDA:EcoCyc.
DR GO; GO:0005524; F:ATP binding; ISM:EcoCyc.
DR GO; GO:0016887; F:ATP hydrolysis activity; IEA:InterPro.
DR GO; GO:0042626; F:ATPase-coupled transmembrane transporter activity; IBA:GO_Central.
DR GO; GO:0015889; P:cobalamin transport; IDA:EcoCyc.
DR Gene3D; 3.40.50.300; -; 1.
DR HAMAP; MF_01005; BtuD; 1.
DR InterPro; IPR003593; AAA+_ATPase.
DR InterPro; IPR003439; ABC_transporter-like_ATP-bd.
DR InterPro; IPR017871; ABC_transporter-like_CS.
DR InterPro; IPR023693; ABC_transptr_BtuD.
DR InterPro; IPR027417; P-loop_NTPase.
DR Pfam; PF00005; ABC_tran; 1.
DR SMART; SM00382; AAA; 1.
DR SUPFAM; SSF52540; SSF52540; 1.
DR PROSITE; PS00211; ABC_TRANSPORTER_1; 1.
DR PROSITE; PS50893; ABC_TRANSPORTER_2; 1.
PE 1: Evidence at protein level;
KW 3D-structure; ATP-binding; Cell inner membrane; Cell membrane; Membrane;
KW Nucleotide-binding; Reference proteome; Translocase; Transport.
FT CHAIN 1..249
FT /note="Vitamin B12 import ATP-binding protein BtuD"
FT /id="PRO_0000091950"
FT DOMAIN 1..233
FT /note="ABC transporter"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01005"
FT BINDING 33..40
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01005"
FT STRAND 3..12
FT /evidence="ECO:0007829|PDB:2QI9"
FT TURN 13..15
FT /evidence="ECO:0007829|PDB:2QI9"
FT STRAND 16..24
FT /evidence="ECO:0007829|PDB:2QI9"
FT STRAND 28..32
FT /evidence="ECO:0007829|PDB:2QI9"
FT HELIX 39..46
FT /evidence="ECO:0007829|PDB:2QI9"
FT STRAND 53..58
FT /evidence="ECO:0007829|PDB:2QI9"
FT HELIX 63..65
FT /evidence="ECO:0007829|PDB:2QI9"
FT HELIX 68..74
FT /evidence="ECO:0007829|PDB:2QI9"
FT STRAND 75..78
FT /evidence="ECO:0007829|PDB:2QI9"
FT HELIX 90..95
FT /evidence="ECO:0007829|PDB:2QI9"
FT HELIX 104..113
FT /evidence="ECO:0007829|PDB:2QI9"
FT HELIX 117..119
FT /evidence="ECO:0007829|PDB:2QI9"
FT STRAND 120..123
FT /evidence="ECO:0007829|PDB:4DBL"
FT HELIX 124..126
FT /evidence="ECO:0007829|PDB:2QI9"
FT HELIX 129..144
FT /evidence="ECO:0007829|PDB:2QI9"
FT TURN 146..148
FT /evidence="ECO:0007829|PDB:2QI9"
FT STRAND 154..159
FT /evidence="ECO:0007829|PDB:2QI9"
FT TURN 160..163
FT /evidence="ECO:0007829|PDB:2QI9"
FT HELIX 166..181
FT /evidence="ECO:0007829|PDB:2QI9"
FT STRAND 185..189
FT /evidence="ECO:0007829|PDB:2QI9"
FT HELIX 193..199
FT /evidence="ECO:0007829|PDB:2QI9"
FT STRAND 201..207
FT /evidence="ECO:0007829|PDB:2QI9"
FT STRAND 210..216
FT /evidence="ECO:0007829|PDB:2QI9"
FT HELIX 217..220
FT /evidence="ECO:0007829|PDB:2QI9"
FT HELIX 223..230
FT /evidence="ECO:0007829|PDB:2QI9"
FT STRAND 234..239
FT /evidence="ECO:0007829|PDB:2QI9"
FT STRAND 242..247
FT /evidence="ECO:0007829|PDB:2QI9"
SQ SEQUENCE 249 AA; 27081 MW; 760004A5C0134245 CRC64;
MSIVMQLQDV AESTRLGPLS GEVRAGEILH LVGPNGAGKS TLLARMAGMT SGKGSIQFAG
QPLEAWSATK LALHRAYLSQ QQTPPFATPV WHYLTLHQHD KTRTELLNDV AGALALDDKL
GRSTNQLSGG EWQRVRLAAV VLQITPQANP AGQLLLLDEP MNSLDVAQQS ALDKILSALC
QQGLAIVMSS HDLNHTLRHA HRAWLLKGGK MLASGRREEV LTPPNLAQAY GMNFRRLDIE
GHRMLISTI
