ID   TPMT_PSEU5              Reviewed;         235 AA.
AC   Q93JT2; A4VJ56;
DT   13-SEP-2005, integrated into UniProtKB/Swiss-Prot.
DT   13-SEP-2005, sequence version 3.
DT   03-AUG-2022, entry version 111.
DE   RecName: Full=Thiopurine S-methyltransferase {ECO:0000255|HAMAP-Rule:MF_00812};
DE            EC=2.1.1.67 {ECO:0000255|HAMAP-Rule:MF_00812};
DE   AltName: Full=Thiopurine methyltransferase {ECO:0000255|HAMAP-Rule:MF_00812};
GN   Name=tpm {ECO:0000255|HAMAP-Rule:MF_00812}; Synonyms=tmpA;
GN   OrderedLocusNames=PST_1312;
OS   Pseudomonas stutzeri (strain A1501).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Pseudomonadales;
OC   Pseudomonadaceae; Pseudomonas.
OX   NCBI_TaxID=379731;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=12904565; DOI=10.1099/mic.0.26270-0;
RA   Desnoues N., Lin M., Guo X., Ma L., Carreno-Lopez R., Elmerich C.;
RT   "Nitrogen fixation genetics and regulation in a Pseudomonas stutzeri strain
RT   associated with rice.";
RL   Microbiology 149:2251-2262(2003).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=A1501;
RX   PubMed=18495935; DOI=10.1073/pnas.0801093105;
RA   Yan Y., Yang J., Dou Y., Chen M., Ping S., Peng J., Lu W., Zhang W.,
RA   Yao Z., Li H., Liu W., He S., Geng L., Zhang X., Yang F., Yu H., Zhan Y.,
RA   Li D., Lin Z., Wang Y., Elmerich C., Lin M., Jin Q.;
RT   "Nitrogen fixation island and rhizosphere competence traits in the genome
RT   of root-associated Pseudomonas stutzeri A1501.";
RL   Proc. Natl. Acad. Sci. U.S.A. 105:7564-7569(2008).
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=S-adenosyl-L-methionine + a thiopurine = S-adenosyl-L-
CC         homocysteine + a thiopurine S-methylether.; EC=2.1.1.67;
CC         Evidence={ECO:0000255|HAMAP-Rule:MF_00812};
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_00812}.
CC   -!- SIMILARITY: Belongs to the class I-like SAM-binding methyltransferase
CC       superfamily. TPMT family. {ECO:0000255|HAMAP-Rule:MF_00812}.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=CAC44176.3; Type=Erroneous initiation; Evidence={ECO:0000305};
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DR   EMBL; AJ297529; CAC44176.3; ALT_INIT; Genomic_DNA.
DR   EMBL; CP000304; ABP79007.1; -; Genomic_DNA.
DR   RefSeq; WP_011912491.1; NC_009434.1.
DR   AlphaFoldDB; Q93JT2; -.
DR   SMR; Q93JT2; -.
DR   STRING; 379731.PST_1312; -.
DR   EnsemblBacteria; ABP79007; ABP79007; PST_1312.
DR   KEGG; psa:PST_1312; -.
DR   eggNOG; COG2265; Bacteria.
DR   HOGENOM; CLU_085515_1_0_6; -.
DR   OMA; ELWIREF; -.
DR   Proteomes; UP000000233; Chromosome.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0008119; F:thiopurine S-methyltransferase activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0032259; P:methylation; IEA:UniProtKB-KW.
DR   Gene3D; 3.40.50.150; -; 1.
DR   HAMAP; MF_00812; Thiopur_methtran; 1.
DR   InterPro; IPR029063; SAM-dependent_MTases_sf.
DR   InterPro; IPR025835; Thiopurine_S-MeTrfase.
DR   InterPro; IPR008854; TPMT.
DR   Pfam; PF05724; TPMT; 1.
DR   PIRSF; PIRSF023956; Thiopurine_S-methyltransferase; 1.
DR   SUPFAM; SSF53335; SSF53335; 1.
DR   PROSITE; PS51585; SAM_MT_TPMT; 1.
PE   3: Inferred from homology;
KW   Cytoplasm; Methyltransferase; Reference proteome; S-adenosyl-L-methionine;
KW   Transferase.
FT   CHAIN           1..235
FT                   /note="Thiopurine S-methyltransferase"
FT                   /id="PRO_0000220131"
FT   REGION          199..235
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   BINDING         13
FT                   /ligand="S-adenosyl-L-methionine"
FT                   /ligand_id="ChEBI:CHEBI:59789"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00812"
FT   BINDING         48
FT                   /ligand="S-adenosyl-L-methionine"
FT                   /ligand_id="ChEBI:CHEBI:59789"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00812"
FT   BINDING         69
FT                   /ligand="S-adenosyl-L-methionine"
FT                   /ligand_id="ChEBI:CHEBI:59789"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00812"
FT   BINDING         126
FT                   /ligand="S-adenosyl-L-methionine"
FT                   /ligand_id="ChEBI:CHEBI:59789"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00812"
SQ   SEQUENCE   235 AA;  26502 MW;  410021AD19465711 CRC64;
     MTDHDNQRWL QLWRERRTDF HQHGVNLLLS RFWPAFAPAT PSRVFVPLCG KSLDMLWLAE
     QGHDVIGVEL SPLAIEAFFR ENHLPPSKRR QGRFTLWRHG RIGILCGDYF ALSEADLGPV
     DSVYDRAALT ALPPILRSRY VAQLRRIVPD TARVFLLTLE DAEADATLQQ ALGVDEELAA
     LYTAGFEIAL AHVESLFEPD PQNGAPRRVE HKVYQLTGKR PASPEADGRA AETED