ID   TPM_MACRS               Reviewed;         284 AA.
AC   V5NBV4; D3XNR9;
DT   25-MAY-2022, integrated into UniProtKB/Swiss-Prot.
DT   19-FEB-2014, sequence version 1.
DT   03-AUG-2022, entry version 13.
DE   RecName: Full=Tropomyosin Mac r 1.0101 {ECO:0000303|PubMed:27001653};
DE   AltName: Full=Major allergen Mac r 1.0101 {ECO:0000303|PubMed:27001653};
DE   AltName: Allergen=Mac r 1.0101 {ECO:0000303|PubMed:27001653};
OS   Macrobrachium rosenbergii (Giant fresh water prawn).
OC   Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Crustacea; Multicrustacea;
OC   Malacostraca; Eumalacostraca; Eucarida; Decapoda; Pleocyemata; Caridea;
OC   Palaemonoidea; Palaemonidae; Macrobrachium.
OX   NCBI_TaxID=79674 {ECO:0000312|EMBL:AHA85706.1};
RN   [1] {ECO:0000312|EMBL:AHA85706.1}
RP   NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY, ALLERGEN, AND CIRCULAR
RP   DICHROISM ANALYSIS.
RC   TISSUE=Muscle {ECO:0000303|PubMed:27001653, ECO:0000312|EMBL:AHA85706.1};
RX   PubMed=27001653; DOI=10.12932/ap0698;
RA   Kumjim S., Jirapongsananuruk O., Piboonpocanun S.;
RT   "Cloning and characterization of recombinant tropomyosin of giant
RT   freshwater shrimp M. rosenbergii to determine major allergens causing
RT   allergic reactions among shrimp-allergic children.";
RL   Asian Pac. J. Allergy Immunol. 34:229-235(2016).
RN   [2] {ECO:0000312|EMBL:ADC55380.1}
RP   NUCLEOTIDE SEQUENCE [MRNA], AND VARIANT 44-PHE-SER-45.
RA   Phiriyangkul P., Phiphobmongkol C., Punyarit P.;
RT   "Molecular cloning and sequencing of tropomyosin from Macrobrachium
RT   rosenbergii.";
RL   Submitted (JUN-2011) to the EMBL/GenBank/DDBJ databases.
RN   [3]
RP   PROTEIN SEQUENCE OF 22-35 AND 113-125, TISSUE SPECIFICITY, IDENTIFICATION
RP   BY MASS SPECTROMETRY, ALLERGEN, AND PHYLOGENETIC ANALYSIS.
RX   PubMed=34255496; DOI=10.1021/acs.jafc.1c00934;
RA   Laurchan P., E-Kobon T., Srisapoome P., Unajak S., Sinthuvanich C.;
RT   "Molecular Characterization and Cross-Allergenicity of Tropomyosin from
RT   Freshwater Crustaceans.";
RL   J. Agric. Food Chem. 69:8247-8256(2021).
RN   [4]
RP   IDENTIFICATION BY MASS SPECTROMETRY, AND ALLERGEN.
RX   PubMed=23569834; DOI=10.1016/s2221-1691(11)60189-5;
RA   Yadzir Z.H., Misnan R., Abdullah N., Bakhtiar F., Arip M., Murad S.;
RT   "Identification of the major allergen of Macrobrachium rosenbergii (giant
RT   freshwater prawn).";
RL   Asian Pac. J. Trop. Biomed. 2:50-54(2012).
CC   -!- FUNCTION: Tropomyosin, in association with the troponin complex, plays
CC       a central role in the calcium dependent regulation of muscle
CC       contraction. {ECO:0000250|UniProtKB:Q22866}.
CC   -!- SUBUNIT: Homodimer. {ECO:0000250|UniProtKB:A2V735}.
CC   -!- TISSUE SPECIFICITY: Expressed in muscle (at protein level)
CC       (PubMed:34255496). Expressed in abdominal muscle (PubMed:27001653).
CC       {ECO:0000269|PubMed:27001653, ECO:0000269|PubMed:34255496}.
CC   -!- DOMAIN: The molecule is in a coiled coil structure that is formed by 2
CC       polypeptide chains. The sequence exhibits a prominent seven-residues
CC       periodicity. {ECO:0000305}.
CC   -!- ALLERGEN: Causes an allergic reaction in human. Natural protein binds
CC       to IgE in patients allergic to giant fresh water prawn (M.rosenbergii)
CC       (PubMed:27001653, PubMed:23569834, PubMed:34255496). Recombinant
CC       protein binds to IgE in 77% of the 13 Thai children tested allergic to
CC       both seawater prawn P.monodon and freshwater prawn M.rosenbergii.
CC       Cross-reacts with P.monodon tropomyosin allergen Pen m 1
CC       (PubMed:27001653). Cross-reacts with tropomyosins of freshwater
CC       crustaceans M.lanchesteri, C.quadricarinatus and P.clarkii
CC       (PubMed:34255496). {ECO:0000269|PubMed:23569834,
CC       ECO:0000269|PubMed:27001653, ECO:0000269|PubMed:34255496}.
CC   -!- SIMILARITY: Belongs to the tropomyosin family.
CC       {ECO:0000255|RuleBase:RU004515}.
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DR   EMBL; KF571722; AHA85706.1; -; mRNA.
DR   EMBL; GU369816; ADC55380.1; -; mRNA.
DR   Allergome; 8806; Mac r 1.
DR   Allergome; 8807; Mac r 1.0101.
DR   InterPro; IPR000533; Tropomyosin.
DR   Pfam; PF00261; Tropomyosin; 1.
DR   PRINTS; PR00194; TROPOMYOSIN.
DR   PROSITE; PS00326; TROPOMYOSIN; 1.
PE   1: Evidence at protein level;
KW   Acetylation; Allergen; Coiled coil; Direct protein sequencing;
KW   Muscle protein; Repeat.
FT   CHAIN           1..284
FT                   /note="Tropomyosin Mac r 1.0101"
FT                   /id="PRO_0000455697"
FT   REGION          1..42
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COILED          1..273
FT                   /evidence="ECO:0000255"
FT   MOD_RES         1
FT                   /note="N-acetylmethionine"
FT                   /evidence="ECO:0000250|UniProtKB:A1KYZ2"
FT   VARIANT         44..45
FT                   /note="FS -> HN"
FT                   /evidence="ECO:0000269|Ref.2"
SQ   SEQUENCE   284 AA;  32829 MW;  8ACA4C565D73B144 CRC64;
     MDAIKKKMQA MKLEKDNAMD RADTLEQQNK EANNRAEKSE EEVFSLQKRM QQLENDLDSV
     QEALLKANQH LEEKDKALSN AEGEVAALNR RIQLLEEDLE RSEERLNTAT TKLAEASQAA
     DESERMRKVL ENRSLSDEER MDALENQLKE ARFLAEEADR KYDEVARKLA MVEADLERAE
     ERAETGESKI VELEEELRVV GNNLKSLEVS EEKANQREEA YKEQIKTLTN KLKAAEARAE
     FAERSVQKLQ KEVDRLEDEL VNEKEKYKSI TDELDQTFSE LSGY