TPN1_YEAST
ID TPN1_YEAST Reviewed; 579 AA.
AC P53099; D6VTW8;
DT 01-OCT-1996, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-1996, sequence version 1.
DT 03-AUG-2022, entry version 143.
DE RecName: Full=Vitamin B6 transporter TPN1;
DE AltName: Full=Transport of pyridoxine protein 1;
GN Name=TPN1; OrderedLocusNames=YGL186C; ORFNames=G1370;
OS Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC Saccharomycetales; Saccharomycetaceae; Saccharomyces.
OX NCBI_TaxID=559292;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, AND SUBCELLULAR LOCATION.
RC STRAIN=ATCC 208353 / W303-1A;
RX PubMed=12649274; DOI=10.1074/jbc.m300949200;
RA Stolz J., Vielreicher M.;
RT "Tpn1p, the plasma membrane vitamin B6 transporter of Saccharomyces
RT cerevisiae.";
RL J. Biol. Chem. 278:18990-18996(2003).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=ATCC 96604 / S288c / FY1679;
RX PubMed=9046087;
RX DOI=10.1002/(sici)1097-0061(199701)13:1<55::aid-yea48>3.0.co;2-9;
RA Coglievina M., Klima R., Bertani I., Delneri D., Zaccaria P., Bruschi C.V.;
RT "Sequencing of a 40.5 kb fragment located on the left arm of chromosome VII
RT from Saccharomyces cerevisiae.";
RL Yeast 13:55-64(1997).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=9169869;
RA Tettelin H., Agostoni-Carbone M.L., Albermann K., Albers M., Arroyo J.,
RA Backes U., Barreiros T., Bertani I., Bjourson A.J., Brueckner M.,
RA Bruschi C.V., Carignani G., Castagnoli L., Cerdan E., Clemente M.L.,
RA Coblenz A., Coglievina M., Coissac E., Defoor E., Del Bino S., Delius H.,
RA Delneri D., de Wergifosse P., Dujon B., Durand P., Entian K.-D., Eraso P.,
RA Escribano V., Fabiani L., Fartmann B., Feroli F., Feuermann M.,
RA Frontali L., Garcia-Gonzalez M., Garcia-Saez M.I., Goffeau A.,
RA Guerreiro P., Hani J., Hansen M., Hebling U., Hernandez K., Heumann K.,
RA Hilger F., Hofmann B., Indge K.J., James C.M., Klima R., Koetter P.,
RA Kramer B., Kramer W., Lauquin G., Leuther H., Louis E.J., Maillier E.,
RA Marconi A., Martegani E., Mazon M.J., Mazzoni C., McReynolds A.D.K.,
RA Melchioretto P., Mewes H.-W., Minenkova O., Mueller-Auer S., Nawrocki A.,
RA Netter P., Neu R., Nombela C., Oliver S.G., Panzeri L., Paoluzi S.,
RA Plevani P., Portetelle D., Portillo F., Potier S., Purnelle B., Rieger M.,
RA Riles L., Rinaldi T., Robben J., Rodrigues-Pousada C.,
RA Rodriguez-Belmonte E., Rodriguez-Torres A.M., Rose M., Ruzzi M.,
RA Saliola M., Sanchez-Perez M., Schaefer B., Schaefer M., Scharfe M.,
RA Schmidheini T., Schreer A., Skala J., Souciet J.-L., Steensma H.Y.,
RA Talla E., Thierry A., Vandenbol M., van der Aart Q.J.M., Van Dyck L.,
RA Vanoni M., Verhasselt P., Voet M., Volckaert G., Wambutt R., Watson M.D.,
RA Weber N., Wedler E., Wedler H., Wipfli P., Wolf K., Wright L.F.,
RA Zaccaria P., Zimmermann M., Zollner A., Kleine K.;
RT "The nucleotide sequence of Saccharomyces cerevisiae chromosome VII.";
RL Nature 387:81-84(1997).
RN [4]
RP GENOME REANNOTATION.
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=24374639; DOI=10.1534/g3.113.008995;
RA Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R.,
RA Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S.,
RA Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.;
RT "The reference genome sequence of Saccharomyces cerevisiae: Then and now.";
RL G3 (Bethesda) 4:389-398(2014).
RN [5]
RP TOPOLOGY [LARGE SCALE ANALYSIS].
RC STRAIN=ATCC 208353 / W303-1A;
RX PubMed=16847258; DOI=10.1073/pnas.0604075103;
RA Kim H., Melen K., Oesterberg M., von Heijne G.;
RT "A global topology map of the Saccharomyces cerevisiae membrane proteome.";
RL Proc. Natl. Acad. Sci. U.S.A. 103:11142-11147(2006).
CC -!- FUNCTION: Thiamine-regulated, high affinity import carrier of
CC pyridoxine, pyridoxal and pyridoxamine. {ECO:0000269|PubMed:12649274}.
CC -!- SUBCELLULAR LOCATION: Membrane {ECO:0000269|PubMed:12649274}; Multi-
CC pass membrane protein {ECO:0000269|PubMed:12649274}.
CC -!- SIMILARITY: Belongs to the purine-cytosine permease (2.A.39) family.
CC {ECO:0000305}.
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DR EMBL; X91489; CAA62788.1; -; Genomic_DNA.
DR EMBL; Z72708; CAA96898.1; -; Genomic_DNA.
DR EMBL; BK006941; DAA07929.1; -; Genomic_DNA.
DR PIR; S61131; S61131.
DR RefSeq; NP_011329.1; NM_001181051.1.
DR AlphaFoldDB; P53099; -.
DR BioGRID; 33069; 60.
DR IntAct; P53099; 1.
DR STRING; 4932.YGL186C; -.
DR TCDB; 2.A.39.2.2; the nucleobase:cation symporter-1 (ncs1) family.
DR iPTMnet; P53099; -.
DR MaxQB; P53099; -.
DR PaxDb; P53099; -.
DR PRIDE; P53099; -.
DR EnsemblFungi; YGL186C_mRNA; YGL186C; YGL186C.
DR GeneID; 852689; -.
DR KEGG; sce:YGL186C; -.
DR SGD; S000003154; TPN1.
DR VEuPathDB; FungiDB:YGL186C; -.
DR eggNOG; ENOG502QR29; Eukaryota.
DR GeneTree; ENSGT00940000176331; -.
DR HOGENOM; CLU_026016_2_1_1; -.
DR InParanoid; P53099; -.
DR OMA; SLCYSIT; -.
DR BioCyc; MetaCyc:G3O-30671-MON; -.
DR BioCyc; YEAST:G3O-30671-MON; -.
DR PRO; PR:P53099; -.
DR Proteomes; UP000002311; Chromosome VII.
DR RNAct; P53099; protein.
DR GO; GO:0000324; C:fungal-type vacuole; HDA:SGD.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0005886; C:plasma membrane; IDA:SGD.
DR GO; GO:0022857; F:transmembrane transporter activity; IBA:GO_Central.
DR GO; GO:0031924; F:vitamin B6 transmembrane transporter activity; IEA:InterPro.
DR GO; GO:0090482; F:vitamin transmembrane transporter activity; IDA:SGD.
DR GO; GO:0051180; P:vitamin transport; IDA:SGD.
DR InterPro; IPR012681; NCS1.
DR InterPro; IPR001248; Pur-cyt_permease.
DR InterPro; IPR026030; Pur-cyt_permease_Fcy2/21/22.
DR InterPro; IPR030175; Tpn1.
DR PANTHER; PTHR31806; PTHR31806; 1.
DR PANTHER; PTHR31806:SF8; PTHR31806:SF8; 1.
DR Pfam; PF02133; Transp_cyt_pur; 1.
DR PIRSF; PIRSF002744; Pur-cyt_permease; 1.
DR TIGRFAMs; TIGR00800; ncs1; 1.
PE 1: Evidence at protein level;
KW Membrane; Reference proteome; Transmembrane; Transmembrane helix;
KW Transport.
FT CHAIN 1..579
FT /note="Vitamin B6 transporter TPN1"
FT /id="PRO_0000197929"
FT TOPO_DOM 1..98
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 99..119
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 120..122
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 123..143
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 144..157
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 158..178
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 179..198
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 199..219
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 220..221
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 222..242
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 243..274
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 275..295
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 296..302
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 303..323
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 324..362
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 363..383
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 384..394
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 395..415
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 416..421
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 422..442
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 443..519
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 520..540
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 541..545
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 546..566
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 567..579
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
SQ SEQUENCE 579 AA; 64546 MW; 26686199D1FD8522 CRC64;
MNRDNMDTTK RKEDHTKHTT DVIEFYEEGT AASSLNIATE KANSSPSILR RIINRAAWLS
KKVDAMGVES TGIQRISPYE RGTSKKQFLH VAGLWLSATG GLSSMSSFLL GPLLFGLSFR
ESVASSLISV TIGCLIAAYC SIMGPQSGCR QMVTARYLFG WWFVKLVALA SIIGVMGWSV
VNSVVGGEML AAISNDKVPL WVGIVIVTVC SFLVAIFGIK QVIKVETYLS VPVLTAFLLL
YISSSDKYSF VNAYVSKGNL DSSTRKGNWM SFFSLCYSIT ATWGSITADY YILFPEDTPY
IQIFCLTFFG TFLPTCFVGI LGLLLASVAM SYKPWSVEYD SHGMGGLLWA GFQRWNGFGK
FCVVVLVFSL VSNNIINTYS AAFSIQLSSV FCAKIPRWFW SIVCTIICLV CALIGRNHFS
TILGNFLPMI GYWISMYFIL LFEENLVFRR FFLHLYTKEF PTVTGEINGP ELVGSSKEVE
KDAVTNIHLL KRKHKVTKHR YNWDKWEDYE VLTHGYAATF AFIVGVAGVV VGMAQAYWIG
PIAAKFGEYG GDVAMWLSMA FSGVVYPPCR YLELRKFGR
