ID   TPN1_YEASX              Reviewed;         579 AA.
AC   Q874L4;
DT   21-MAR-2006, integrated into UniProtKB/Swiss-Prot.
DT   01-JUN-2003, sequence version 1.
DT   25-MAY-2022, entry version 54.
DE   RecName: Full=Vitamin B6 transporter TPN1;
DE   AltName: Full=Transport of pyridoxine protein 1;
GN   Name=TPN1;
OS   Saccharomyces cerevisiae (Baker's yeast).
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC   Saccharomycetales; Saccharomycetaceae; Saccharomyces.
OX   NCBI_TaxID=4932;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=4228 / ATCC 9080 / CBS 2354 / DSM 70424 / NBRC 0565 / NCYC 74 / NRRL
RC   Y-1089;
RX   PubMed=12649274; DOI=10.1074/jbc.m300949200;
RA   Stolz J., Vielreicher M.;
RT   "Tpn1p, the plasma membrane vitamin B6 transporter of Saccharomyces
RT   cerevisiae.";
RL   J. Biol. Chem. 278:18990-18996(2003).
CC   -!- FUNCTION: Thiamine-regulated, high affinity import carrier of
CC       pyridoxine, pyridoxal and pyridoxamine. {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Membrane {ECO:0000305}; Multi-pass membrane
CC       protein {ECO:0000305}.
CC   -!- SIMILARITY: Belongs to the purine-cytosine permease (2.A.39) family.
CC       {ECO:0000305}.
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   EMBL; AY216467; AAO73409.1; -; Genomic_DNA.
DR   AlphaFoldDB; Q874L4; -.
DR   VEuPathDB; FungiDB:YGL186C; -.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0005886; C:plasma membrane; IEA:InterPro.
DR   GO; GO:0031924; F:vitamin B6 transmembrane transporter activity; IEA:InterPro.
DR   InterPro; IPR012681; NCS1.
DR   InterPro; IPR001248; Pur-cyt_permease.
DR   InterPro; IPR026030; Pur-cyt_permease_Fcy2/21/22.
DR   InterPro; IPR030175; Tpn1.
DR   PANTHER; PTHR31806; PTHR31806; 1.
DR   PANTHER; PTHR31806:SF8; PTHR31806:SF8; 1.
DR   Pfam; PF02133; Transp_cyt_pur; 1.
DR   PIRSF; PIRSF002744; Pur-cyt_permease; 1.
DR   TIGRFAMs; TIGR00800; ncs1; 1.
PE   3: Inferred from homology;
KW   Membrane; Transmembrane; Transmembrane helix; Transport.
FT   CHAIN           1..579
FT                   /note="Vitamin B6 transporter TPN1"
FT                   /id="PRO_0000228845"
FT   TRANSMEM        99..119
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        123..143
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        158..178
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        199..219
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        222..242
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        275..295
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        303..323
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        363..383
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        395..415
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        422..442
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        520..540
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        546..566
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   579 AA;  64560 MW;  E10DF0C05A823247 CRC64;
     MNRDNMDTTK RKEDHTKHTT DVIEFYEEGT AASSLNIATE KANSSPSILR RIINRAAWLS
     KKVDAMGVES TGIQRISPYE RGTSKKQFLH VAGLWLSATG GLSSMSSFLL GPLLFGLSFR
     ESLASSLISV TIGCLIAAYC SIMGPQSGCR QMVTARYLFG WWFVKLVALA SIIGVMGWSV
     VNSVVGGEML AAISNDKVPL WVGIVIVTVC SFLVAIFGIK QVIKVETYLS VPVLTAFLLL
     YISSSDKYSF VNAYVSKGNL DSSTRKGNWM SFFSLCYSIT ATWGSITADY YILFPEDTPY
     IQIFCLTFFG TFLPTCFVGI LGLLLASVAM SYKPWSVEYD SHGMGGLLWA GFQRWNGFGK
     FCVVVLVFSL VSNNIINTYS AAFSIQLSSV FCAKIPRWFW SIVCTIICLV CALIGRNHFS
     TILGNFLPMI GYWISMYFIL LFEENLVFRR FFLHLYTKEF PTVTGEINGP ELVGSSKEVE
     KDAVTNIHLL KRKHKVTKHR YNWDKWEDYE VLTHGYAATF AFIVGVAGVV VGMAQAYWIG
     PIAAKFGEYG GDVAMWLSMA FSGVVYPPCR YLELRKFGR