TPO_RAT
ID TPO_RAT Reviewed; 326 AA.
AC P49745;
DT 01-OCT-1996, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-1996, sequence version 1.
DT 03-AUG-2022, entry version 128.
DE RecName: Full=Thrombopoietin;
DE Flags: Precursor;
GN Name=Thpo;
OS Rattus norvegicus (Rat).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Rattus.
OX NCBI_TaxID=10116;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RC TISSUE=Liver;
RX PubMed=7607561; DOI=10.1016/0378-1119(95)00125-p;
RA Ogami K., Shimada Y., Sohma Y., Akahori H., Kato T., Kawamura K.,
RA Miyazaki H.;
RT "The sequence of a rat cDNA encoding thrombopoietin.";
RL Gene 158:309-310(1995).
CC -!- FUNCTION: Lineage-specific cytokine affecting the proliferation and
CC maturation of megakaryocytes from their committed progenitor cells. It
CC acts at a late stage of megakaryocyte development. It may be the major
CC physiological regulator of circulating platelets.
CC -!- SUBCELLULAR LOCATION: Secreted.
CC -!- DOMAIN: Two-domain structure with an erythropoietin-like N-terminal and
CC a Ser/Pro/Thr-rich C-terminal.
CC -!- SIMILARITY: Belongs to the EPO/TPO family. {ECO:0000305}.
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DR EMBL; D32207; BAA06906.1; -; mRNA.
DR PIR; JC4125; JC4125.
DR RefSeq; NP_112395.1; NM_031133.2.
DR RefSeq; XP_008767049.1; XM_008768827.2.
DR RefSeq; XP_008767050.1; XM_008768828.2.
DR RefSeq; XP_017453567.1; XM_017598078.1.
DR RefSeq; XP_017453670.1; XM_017598181.1.
DR AlphaFoldDB; P49745; -.
DR SMR; P49745; -.
DR STRING; 10116.ENSRNOP00000065517; -.
DR GlyGen; P49745; 5 sites.
DR iPTMnet; P49745; -.
DR PhosphoSitePlus; P49745; -.
DR PaxDb; P49745; -.
DR GeneID; 81811; -.
DR KEGG; rno:81811; -.
DR CTD; 81811; -.
DR RGD; 621438; Thpo.
DR VEuPathDB; HostDB:ENSRNOG00000046850; -.
DR eggNOG; ENOG502S9T0; Eukaryota.
DR HOGENOM; CLU_039844_0_0_1; -.
DR InParanoid; P49745; -.
DR OMA; PILCARQ; -.
DR OrthoDB; 906192at2759; -.
DR PhylomeDB; P49745; -.
DR TreeFam; TF338084; -.
DR PRO; PR:P49745; -.
DR Proteomes; UP000002494; Chromosome 11.
DR Bgee; ENSRNOG00000046850; Expressed in liver and 12 other tissues.
DR Genevisible; P49745; RN.
DR GO; GO:0005576; C:extracellular region; IDA:RGD.
DR GO; GO:0005615; C:extracellular space; IEA:UniProtKB-KW.
DR GO; GO:0005125; F:cytokine activity; IEA:UniProtKB-KW.
DR GO; GO:0005179; F:hormone activity; IEA:UniProtKB-KW.
DR GO; GO:0005102; F:signaling receptor binding; IDA:RGD.
DR GO; GO:0008283; P:cell population proliferation; IEA:InterPro.
DR GO; GO:0071222; P:cellular response to lipopolysaccharide; IEP:RGD.
DR GO; GO:0035855; P:megakaryocyte development; ISO:RGD.
DR GO; GO:0030099; P:myeloid cell differentiation; ISO:RGD.
DR GO; GO:0008284; P:positive regulation of cell population proliferation; IDA:RGD.
DR GO; GO:0070374; P:positive regulation of ERK1 and ERK2 cascade; ISS:UniProtKB.
DR GO; GO:1902035; P:positive regulation of hematopoietic stem cell proliferation; ISO:RGD.
DR GO; GO:0043410; P:positive regulation of MAPK cascade; ISO:RGD.
DR GO; GO:0045654; P:positive regulation of megakaryocyte differentiation; ISS:UniProtKB.
DR GO; GO:0051897; P:positive regulation of protein kinase B signaling; ISS:UniProtKB.
DR GO; GO:0001934; P:positive regulation of protein phosphorylation; ISS:UniProtKB.
DR GO; GO:0097696; P:receptor signaling pathway via STAT; ISO:RGD.
DR GO; GO:0032496; P:response to lipopolysaccharide; IEP:RGD.
DR GO; GO:0038163; P:thrombopoietin-mediated signaling pathway; ISS:UniProtKB.
DR Gene3D; 1.20.1250.10; -; 1.
DR InterPro; IPR009079; 4_helix_cytokine-like_core.
DR InterPro; IPR019767; EPO/TPO_CS.
DR InterPro; IPR001323; EPO_TPO.
DR InterPro; IPR003978; Thrombopoietin.
DR PANTHER; PTHR10560; PTHR10560; 1.
DR Pfam; PF00758; EPO_TPO; 1.
DR PRINTS; PR01485; THROMBOPTN.
DR SUPFAM; SSF47266; SSF47266; 1.
DR PROSITE; PS00817; EPO_TPO; 1.
PE 2: Evidence at transcript level;
KW Cytokine; Disulfide bond; Glycoprotein; Hormone; Reference proteome;
KW Secreted; Signal.
FT SIGNAL 1..21
FT /evidence="ECO:0000255"
FT CHAIN 22..326
FT /note="Thrombopoietin"
FT /id="PRO_0000008413"
FT REGION 307..326
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 309..326
FT /note="Pro residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT CARBOHYD 197
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 206
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 235
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 249
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 256
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT DISULFID 28..172
FT /evidence="ECO:0000255"
FT DISULFID 50..106
FT /evidence="ECO:0000255"
SQ SEQUENCE 326 AA; 34556 MW; F99D7F77F896FA2C CRC64;
MELTDLLLVA ILLLTARLTL SSPVPPACDP RLLNKLLRDS YLLHSRLSQC PDVNPLSIPV
LLPAVDFSLG EWKTQTEQSK AQDILGAVSL LLEGVMAARG QLEPSCLSSL LGQLSGQVRL
LLGALQGLLG TQLPPQGRTT AHKDPSALFL SLQQLLRGKV RFLLLVEGPA LCVRRTLPTT
AVPSRTSQLL TLNKFPNRTS GLLETNFSVV ARTAGPGLLN RLQGFRAKII PGQLNQTSGS
LDQIPGYLNG THEPVNGTHG LFAGTSLQTL EAPDVVPGAF NKGSLPLNLQ SGLPPIPSLA
ADGYTLFPPS PTFPTPGSPP QLPPVS
