TPP1_ARATH
ID TPP1_ARATH Reviewed; 340 AA.
AC O04348; Q7DM64;
DT 13-NOV-2007, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1998, sequence version 2.
DT 03-AUG-2022, entry version 152.
DE RecName: Full=Thylakoidal processing peptidase 1, chloroplastic;
DE EC=3.4.21.89;
DE AltName: Full=Signal peptidase I-1;
DE Flags: Precursor;
GN Name=TPP1; OrderedLocusNames=At2g30440; ORFNames=T6B20.20;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, AND SUBCELLULAR LOCATION.
RC STRAIN=cv. Columbia;
RX PubMed=9422718; DOI=10.1074/jbc.273.2.689;
RA Chaal B.K., Mould R.M., Barbrook A.C., Gray J.C., Howe C.J.;
RT "Characterization of a cDNA encoding the thylakoidal processing peptidase
RT from Arabidopsis thaliana. Implications for the origin and catalytic
RT mechanism of the enzyme.";
RL J. Biol. Chem. 273:689-692(1998).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=10617197; DOI=10.1038/45471;
RA Lin X., Kaul S., Rounsley S.D., Shea T.P., Benito M.-I., Town C.D.,
RA Fujii C.Y., Mason T.M., Bowman C.L., Barnstead M.E., Feldblyum T.V.,
RA Buell C.R., Ketchum K.A., Lee J.J., Ronning C.M., Koo H.L., Moffat K.S.,
RA Cronin L.A., Shen M., Pai G., Van Aken S., Umayam L., Tallon L.J.,
RA Gill J.E., Adams M.D., Carrera A.J., Creasy T.H., Goodman H.M.,
RA Somerville C.R., Copenhaver G.P., Preuss D., Nierman W.C., White O.,
RA Eisen J.A., Salzberg S.L., Fraser C.M., Venter J.C.;
RT "Sequence and analysis of chromosome 2 of the plant Arabidopsis thaliana.";
RL Nature 402:761-768(1999).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RX PubMed=14593172; DOI=10.1126/science.1088305;
RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA Ecker J.R.;
RT "Empirical analysis of transcriptional activity in the Arabidopsis
RT genome.";
RL Science 302:842-846(2003).
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RA Cheuk R.F., Chen H., Kim C.J., Shinn P., Ecker J.R.;
RT "Arabidopsis ORF clones.";
RL Submitted (FEB-2005) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: Cleaves the thylakoid-transfer domain from a chloroplast
CC protein. {ECO:0000269|PubMed:9422718}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=Cleavage of hydrophobic, N-terminal signal or leader sequences
CC from secreted and periplasmic proteins.; EC=3.4.21.89;
CC -!- SUBCELLULAR LOCATION: Plastid, chloroplast thylakoid membrane
CC {ECO:0000269|PubMed:9422718}; Single-pass membrane protein
CC {ECO:0000269|PubMed:9422718}. Note=located in the non-appressed
CC lamellae of the thylakoid network. {ECO:0000250}.
CC -!- SIMILARITY: Belongs to the peptidase S26 family. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAB63091.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR EMBL; Y10477; CAA71502.1; -; mRNA.
DR EMBL; U93215; AAB63091.1; ALT_SEQ; Genomic_DNA.
DR EMBL; CP002685; AEC08389.1; -; Genomic_DNA.
DR EMBL; AY128354; AAM91557.1; -; mRNA.
DR EMBL; BT020607; AAW80880.1; -; mRNA.
DR PIR; E84708; E84708.
DR RefSeq; NP_180603.2; NM_128597.3.
DR AlphaFoldDB; O04348; -.
DR SMR; O04348; -.
DR STRING; 3702.AT2G30440.1; -.
DR MEROPS; S26.008; -.
DR PaxDb; O04348; -.
DR PRIDE; O04348; -.
DR EnsemblPlants; AT2G30440.1; AT2G30440.1; AT2G30440.
DR GeneID; 817595; -.
DR Gramene; AT2G30440.1; AT2G30440.1; AT2G30440.
DR KEGG; ath:AT2G30440; -.
DR Araport; AT2G30440; -.
DR TAIR; locus:2064337; AT2G30440.
DR eggNOG; KOG0171; Eukaryota.
DR HOGENOM; CLU_025235_0_0_1; -.
DR InParanoid; O04348; -.
DR OMA; PMFVPKG; -.
DR OrthoDB; 1211147at2759; -.
DR PhylomeDB; O04348; -.
DR PRO; PR:O04348; -.
DR Proteomes; UP000006548; Chromosome 2.
DR ExpressionAtlas; O04348; baseline and differential.
DR Genevisible; O04348; AT.
DR GO; GO:0009534; C:chloroplast thylakoid; IDA:TAIR.
DR GO; GO:0009535; C:chloroplast thylakoid membrane; IBA:GO_Central.
DR GO; GO:0005887; C:integral component of plasma membrane; IBA:GO_Central.
DR GO; GO:0004175; F:endopeptidase activity; IDA:TAIR.
DR GO; GO:0004252; F:serine-type endopeptidase activity; IBA:GO_Central.
DR GO; GO:0006465; P:signal peptide processing; IDA:TAIR.
DR GO; GO:0010027; P:thylakoid membrane organization; IBA:GO_Central.
DR CDD; cd06530; S26_SPase_I; 1.
DR InterPro; IPR036286; LexA/Signal_pep-like_sf.
DR InterPro; IPR000223; Pept_S26A_signal_pept_1.
DR InterPro; IPR019758; Pept_S26A_signal_pept_1_CS.
DR InterPro; IPR019756; Pept_S26A_signal_pept_1_Ser-AS.
DR InterPro; IPR019533; Peptidase_S26.
DR PANTHER; PTHR43390; PTHR43390; 1.
DR Pfam; PF10502; Peptidase_S26; 1.
DR PRINTS; PR00727; LEADERPTASE.
DR SUPFAM; SSF51306; SSF51306; 1.
DR TIGRFAMs; TIGR02227; sigpep_I_bact; 1.
DR PROSITE; PS00501; SPASE_I_1; 1.
DR PROSITE; PS00761; SPASE_I_3; 1.
PE 2: Evidence at transcript level;
KW Chloroplast; Hydrolase; Membrane; Plastid; Protease; Reference proteome;
KW Thylakoid; Transit peptide; Transmembrane; Transmembrane helix.
FT TRANSIT 1..52
FT /note="Chloroplast"
FT /evidence="ECO:0000255"
FT TRANSIT 53..?
FT /note="Thylakoid"
FT /evidence="ECO:0000255"
FT CHAIN ?..340
FT /note="Thylakoidal processing peptidase 1, chloroplastic"
FT /id="PRO_0000310734"
FT TRANSMEM 155..175
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 176..340
FT /note="Lumenal, thylakoid"
FT /evidence="ECO:0000255"
FT ACT_SITE 184
FT /evidence="ECO:0000250"
SQ SEQUENCE 340 AA; 37853 MW; F6763EBFC1DAA2E1 CRC64;
MAIRITFTYS THVARNLVGT RVGPGGYCFE SLVRPRFFSH KRDFDRSPRN RPASMYGSIA
RELIGEGSQS PLVMGLISIL KSTTGHESST MNVLGVSSFK ASSIIPFLQG SKWIKNPPVI
DDVDKGGTVC DDDDDKESRN GGSGWVNKLL SVCSEDAKAA FTAVTVSILF RSALAEPKSI
PSTSMYPTLD KGDRVMAEKV SYFFRKPEVS DIVIFKAPPI LLEYPEYGYS SNDVFIKRIV
ASEGDWVEVR DGKLFVNDIV QEEDFVLEPM SYEMEPMFVP KGYVFVLGDN RNKSFDSHNW
GPLPIENIVG RSVFRYWPPS KVSDTIYHDQ AITRGPVAVS
