ID   TPP1_SACMI              Reviewed;         245 AA.
AC   Q9HET9;
DT   31-AUG-2004, integrated into UniProtKB/Swiss-Prot.
DT   01-MAR-2001, sequence version 1.
DT   25-MAY-2022, entry version 57.
DE   RecName: Full=Polynucleotide 3'-phosphatase;
DE            EC=3.1.3.32;
DE   AltName: Full=2'(3')-polynucleotidase;
DE   AltName: Full=DNA 3'-phosphatase;
DE   AltName: Full=Three prime phosphatase;
GN   Name=TPP1;
OS   Saccharomyces mikatae (Yeast).
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC   Saccharomycetales; Saccharomycetaceae; Saccharomyces.
OX   NCBI_TaxID=114525;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=ATCC MYA-4448 / CBS 8839 / NBRC 1815 / NCYC 2888;
RX   PubMed=11278831; DOI=10.1074/jbc.m011075200;
RA   Vance J.R., Wilson T.E.;
RT   "Uncoupling of 3'-phosphatase and 5'-kinase functions in budding yeast.
RT   Characterization of Saccharomyces cerevisiae DNA 3'-phosphatase (TPP1).";
RL   J. Biol. Chem. 276:15073-15081(2001).
CC   -!- FUNCTION: Dephosphorylate DNA's 3'-phosphate termini. Has a role in the
CC       repair of breaks in single-stranded DNA.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=a 3'end (2'-deoxyribonucleotide 3'-phosphate)-DNA + H2O = a
CC         3'-end 2'-deoxyribonucleotide-DNA + phosphate; Xref=Rhea:RHEA:14113,
CC         Rhea:RHEA-COMP:13863, Rhea:RHEA-COMP:13864, ChEBI:CHEBI:15377,
CC         ChEBI:CHEBI:43474, ChEBI:CHEBI:138147, ChEBI:CHEBI:138148;
CC         EC=3.1.3.32;
CC   -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000305}.
CC   -!- SIMILARITY: Belongs to the DNA 3' phosphatase family. {ECO:0000305}.
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DR   EMBL; AF326782; AAG45938.1; -; Genomic_DNA.
DR   AlphaFoldDB; Q9HET9; -.
DR   SMR; Q9HET9; -.
DR   BRENDA; 3.1.3.32; 6902.
DR   GO; GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
DR   GO; GO:0046403; F:polynucleotide 3'-phosphatase activity; IEA:UniProtKB-EC.
DR   GO; GO:0006281; P:DNA repair; IEA:UniProtKB-KW.
DR   Gene3D; 3.40.50.1000; -; 1.
DR   InterPro; IPR036412; HAD-like_sf.
DR   InterPro; IPR023214; HAD_sf.
DR   InterPro; IPR013954; PNK3P.
DR   InterPro; IPR006551; Polynucleotide_phosphatase.
DR   Pfam; PF08645; PNK3P; 1.
DR   SUPFAM; SSF56784; SSF56784; 1.
DR   TIGRFAMs; TIGR01664; DNA-3'-Pase; 1.
PE   3: Inferred from homology;
KW   DNA damage; DNA repair; Hydrolase; Nucleus.
FT   CHAIN           1..245
FT                   /note="Polynucleotide 3'-phosphatase"
FT                   /id="PRO_0000065579"
SQ   SEQUENCE   245 AA;  28144 MW;  4C58256B8B1149EA CRC64;
     MKTEGRQMPH KSTILPFLIK FAPNSPQFTY DCDQCLNVYA FDLDHTIIKP KSPNAKYSRS
     ANDWQFMNFD SKKSTLDYLF SITDNDLAAV IVIFSNQGGV ITVPRTSKSC SKYINKISLF
     LKAIENDKRG EKLSPRLWIY AAPKRPKTVV TNNKKITFPS LCKSYNNDPE IFEKVRKPMT
     GMADFFRIDI TDACRVLKTV PPIKLNWVYY CGDAAGRKND FSDSDIKFAE KLHVEFKYPE
     EVFQG