TPP2_ARATH
ID TPP2_ARATH Reviewed; 367 AA.
AC Q9M9Z2; Q8LEC9;
DT 13-NOV-2007, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-2000, sequence version 1.
DT 03-AUG-2022, entry version 140.
DE RecName: Full=Probable thylakoidal processing peptidase 2, chloroplastic;
DE EC=3.4.21.89;
DE AltName: Full=Signal peptidase I-2;
DE Flags: Precursor;
GN Name=TPP2; OrderedLocusNames=At1g06870; ORFNames=F4H5.6;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=11130712; DOI=10.1038/35048500;
RA Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O.,
RA Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E.,
RA Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K., Conn L.,
RA Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P.,
RA Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D.,
RA Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J.,
RA Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L.,
RA Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A.,
RA Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A.,
RA Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M.,
RA Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M.,
RA Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P.,
RA Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D.,
RA Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D.,
RA Yu G., Fraser C.M., Venter J.C., Davis R.W.;
RT "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana.";
RL Nature 408:816-820(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RA Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B.,
RA Feldmann K.A.;
RT "Full-length cDNA from Arabidopsis thaliana.";
RL Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases.
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RA Shinn P., Chen H., Kim C.J., Ecker J.R.;
RT "Arabidopsis ORF clones.";
RL Submitted (MAR-2006) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: Cleaves the thylakoid-transfer domain from a chloroplast
CC protein. {ECO:0000250}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=Cleavage of hydrophobic, N-terminal signal or leader sequences
CC from secreted and periplasmic proteins.; EC=3.4.21.89;
CC -!- SUBCELLULAR LOCATION: Plastid, chloroplast thylakoid membrane; Single-
CC pass membrane protein. Note=located in the non-appressed lamellae of
CC the thylakoid network. {ECO:0000250}.
CC -!- SIMILARITY: Belongs to the peptidase S26 family. {ECO:0000305}.
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DR EMBL; AC011001; AAF63136.1; -; Genomic_DNA.
DR EMBL; CP002684; AEE28047.1; -; Genomic_DNA.
DR EMBL; AY085489; AAM62715.1; -; mRNA.
DR EMBL; BT024745; ABD59083.1; -; mRNA.
DR PIR; E86203; E86203.
DR RefSeq; NP_172171.1; NM_100563.4.
DR AlphaFoldDB; Q9M9Z2; -.
DR SMR; Q9M9Z2; -.
DR STRING; 3702.AT1G06870.1; -.
DR ChEMBL; CHEMBL1932907; -.
DR MEROPS; S26.A01; -.
DR iPTMnet; Q9M9Z2; -.
DR PaxDb; Q9M9Z2; -.
DR PRIDE; Q9M9Z2; -.
DR ProteomicsDB; 234446; -.
DR EnsemblPlants; AT1G06870.1; AT1G06870.1; AT1G06870.
DR GeneID; 837198; -.
DR Gramene; AT1G06870.1; AT1G06870.1; AT1G06870.
DR KEGG; ath:AT1G06870; -.
DR Araport; AT1G06870; -.
DR TAIR; locus:2033108; AT1G06870.
DR eggNOG; KOG0171; Eukaryota.
DR HOGENOM; CLU_025235_0_0_1; -.
DR InParanoid; Q9M9Z2; -.
DR OMA; ISVMKLT; -.
DR OrthoDB; 1211147at2759; -.
DR PhylomeDB; Q9M9Z2; -.
DR PRO; PR:Q9M9Z2; -.
DR Proteomes; UP000006548; Chromosome 1.
DR ExpressionAtlas; Q9M9Z2; baseline and differential.
DR Genevisible; Q9M9Z2; AT.
DR GO; GO:0009535; C:chloroplast thylakoid membrane; IBA:GO_Central.
DR GO; GO:0005887; C:integral component of plasma membrane; IBA:GO_Central.
DR GO; GO:0004252; F:serine-type endopeptidase activity; IBA:GO_Central.
DR GO; GO:0006465; P:signal peptide processing; IBA:GO_Central.
DR GO; GO:0010027; P:thylakoid membrane organization; IBA:GO_Central.
DR CDD; cd06530; S26_SPase_I; 1.
DR InterPro; IPR036286; LexA/Signal_pep-like_sf.
DR InterPro; IPR000223; Pept_S26A_signal_pept_1.
DR InterPro; IPR019758; Pept_S26A_signal_pept_1_CS.
DR InterPro; IPR019756; Pept_S26A_signal_pept_1_Ser-AS.
DR InterPro; IPR019533; Peptidase_S26.
DR PANTHER; PTHR43390; PTHR43390; 1.
DR Pfam; PF10502; Peptidase_S26; 1.
DR PRINTS; PR00727; LEADERPTASE.
DR SUPFAM; SSF51306; SSF51306; 1.
DR TIGRFAMs; TIGR02227; sigpep_I_bact; 1.
DR PROSITE; PS00501; SPASE_I_1; 1.
DR PROSITE; PS00761; SPASE_I_3; 1.
PE 2: Evidence at transcript level;
KW Chloroplast; Hydrolase; Membrane; Plastid; Protease; Reference proteome;
KW Thylakoid; Transit peptide; Transmembrane; Transmembrane helix.
FT TRANSIT 1..68
FT /note="Chloroplast"
FT /evidence="ECO:0000255"
FT TRANSIT 69..?
FT /note="Thylakoid"
FT /evidence="ECO:0000255"
FT CHAIN ?..367
FT /note="Probable thylakoidal processing peptidase 2,
FT chloroplastic"
FT /id="PRO_0000310735"
FT TRANSMEM 185..205
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 206..367
FT /note="Lumenal, thylakoid"
FT /evidence="ECO:0000255"
FT REGION 53..72
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT ACT_SITE 214
FT /evidence="ECO:0000250"
FT CONFLICT 111
FT /note="M -> V (in Ref. 3; AAM62715)"
FT /evidence="ECO:0000305"
FT CONFLICT 196
FT /note="V -> F (in Ref. 3; AAM62715)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 367 AA; 40150 MW; BE18813DE031BBAE CRC64;
MAIRVTFTYS SYVARSIASS AGTRVGTGDV RSCFETWVRP RFCGHNQIPD IVDKSPGSNT
WGPSSGPRAR PASSMYSTIA REILEEGCKS PLVLGMISLM NLTGAPQFSG MTGLGISPFK
TSSVIPFLRG SKWMPCSIPA TLSTDIAEVD RGGKVCDPKV KLELSDKVSN GGNGWVNKLL
NICSEDAKAA FTAVTVSLLF RSALAEPKSI PSTSMLPTLD VGDRVIAEKV SYFFRKPEVS
DIVIFKAPPI LVEHGYSCAD VFIKRIVASE GDWVEVCDGK LLVNDTVQAE DFVLEPIDYE
MEPMFVPEGY VFVLGDNRNK SFDSHNWGPL PIKNIIGRSV FRYWPPSKVS DIIHHEQVSQ
KRAVDVS
