TPPA_ARATH
ID TPPA_ARATH Reviewed; 385 AA.
AC O64896; Q8RWE2;
DT 13-JUN-2012, integrated into UniProtKB/Swiss-Prot.
DT 01-AUG-1998, sequence version 1.
DT 03-AUG-2022, entry version 134.
DE RecName: Full=Trehalose-phosphate phosphatase A;
DE Short=AtTPPA;
DE EC=3.1.3.12;
DE AltName: Full=Trehalose 6-phosphate phosphatase;
GN Name=TPPA; OrderedLocusNames=At5g51460; ORFNames=K17N15.1, MFG13.17;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), FUNCTION, CATALYTIC ACTIVITY, AND
RP TISSUE SPECIFICITY.
RC STRAIN=cv. Landsberg erecta;
RX PubMed=9681009; DOI=10.1046/j.1365-313x.1998.00064.x;
RA Vogel G., Aeschbacher R.A., Muller J., Boller T., Wiemken A.;
RT "Trehalose-6-phosphate phosphatases from Arabidopsis thaliana:
RT identification by functional complementation of the yeast tps2 mutant.";
RL Plant J. 13:673-683(1998).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=10718197; DOI=10.1093/dnares/7.1.31;
RA Sato S., Nakamura Y., Kaneko T., Katoh T., Asamizu E., Kotani H.,
RA Tabata S.;
RT "Structural analysis of Arabidopsis thaliana chromosome 5. X. Sequence
RT features of the regions of 3,076,755 bp covered by sixty P1 and TAC
RT clones.";
RL DNA Res. 7:31-63(2000).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
RC STRAIN=cv. Columbia;
RX PubMed=14593172; DOI=10.1126/science.1088305;
RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA Ecker J.R.;
RT "Empirical analysis of transcriptional activity in the Arabidopsis
RT genome.";
RL Science 302:842-846(2003).
RN [5]
RP FUNCTION.
RX PubMed=11520870; DOI=10.1093/jexbot/52.362.1817;
RA Vogel G., Fiehn O., Jean-Richard-dit-Bressel L., Boller T., Wiemken A.,
RA Aeschbacher R.A., Wingler A.;
RT "Trehalose metabolism in Arabidopsis: occurrence of trehalose and molecular
RT cloning and characterization of trehalose-6-phosphate synthase
RT homologues.";
RL J. Exp. Bot. 52:1817-1826(2001).
RN [6]
RP GENE FAMILY.
RX PubMed=12508064; DOI=10.1093/jxb/erg039;
RA Eastmond P.J., Li Y., Graham I.A.;
RT "Is trehalose-6-phosphate a regulator of sugar metabolism in plants?";
RL J. Exp. Bot. 54:533-537(2003).
RN [7]
RP INDUCTION, AND NOMENCLATURE.
RX PubMed=15181209; DOI=10.1104/pp.104.039503;
RA Schluepmann H., van Dijken A.J.H., Aghdasi M., Wobbes B., Paul M.,
RA Smeekens S.C.M.;
RT "Trehalose mediated growth inhibition of Arabidopsis seedlings is due to
RT trehalose-6-phosphate accumulation.";
RL Plant Physiol. 135:879-890(2004).
CC -!- FUNCTION: Removes the phosphate from trehalose 6-phosphate to produce
CC free trehalose. Trehalose accumulation in plant may improve abiotic
CC stress tolerance. {ECO:0000269|PubMed:11520870,
CC ECO:0000269|PubMed:9681009}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=alpha,alpha-trehalose 6-phosphate + H2O = alpha,alpha-
CC trehalose + phosphate; Xref=Rhea:RHEA:23420, ChEBI:CHEBI:15377,
CC ChEBI:CHEBI:16551, ChEBI:CHEBI:43474, ChEBI:CHEBI:58429; EC=3.1.3.12;
CC Evidence={ECO:0000269|PubMed:9681009};
CC -!- COFACTOR:
CC Name=a divalent metal cation; Xref=ChEBI:CHEBI:60240;
CC Evidence={ECO:0000250};
CC -!- PATHWAY: Glycan biosynthesis; trehalose biosynthesis.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1;
CC IsoId=O64896-1; Sequence=Displayed;
CC Name=2;
CC IsoId=O64896-2; Sequence=VSP_043862;
CC -!- TISSUE SPECIFICITY: Expressed in flowers. {ECO:0000269|PubMed:9681009}.
CC -!- INDUCTION: By trehalose. {ECO:0000269|PubMed:15181209}.
CC -!- MISCELLANEOUS: [Isoform 2]: May be due to a competing acceptor splice
CC site. {ECO:0000305}.
CC -!- SIMILARITY: Belongs to the trehalose phosphatase family. {ECO:0000305}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; AF007778; AAC39369.1; -; mRNA.
DR EMBL; AB018109; BAB08662.1; -; Genomic_DNA.
DR EMBL; CP002688; AED96085.1; -; Genomic_DNA.
DR EMBL; CP002688; AED96086.1; -; Genomic_DNA.
DR EMBL; CP002688; AED96087.1; -; Genomic_DNA.
DR EMBL; CP002688; ANM70853.1; -; Genomic_DNA.
DR EMBL; CP002688; ANM70854.1; -; Genomic_DNA.
DR EMBL; AY093147; AAM13146.1; -; mRNA.
DR EMBL; BT008467; AAP37826.1; -; mRNA.
DR PIR; T52057; T52057.
DR RefSeq; NP_001332431.1; NM_001344937.1. [O64896-2]
DR RefSeq; NP_001332432.1; NM_001344938.1. [O64896-1]
DR RefSeq; NP_199959.2; NM_124525.2. [O64896-2]
DR RefSeq; NP_851171.1; NM_180840.2. [O64896-1]
DR RefSeq; NP_974922.1; NM_203193.3. [O64896-1]
DR AlphaFoldDB; O64896; -.
DR SMR; O64896; -.
DR STRING; 3702.AT5G51460.1; -.
DR iPTMnet; O64896; -.
DR PaxDb; O64896; -.
DR PRIDE; O64896; -.
DR ProteomicsDB; 232380; -. [O64896-1]
DR EnsemblPlants; AT5G51460.1; AT5G51460.1; AT5G51460. [O64896-1]
DR EnsemblPlants; AT5G51460.2; AT5G51460.2; AT5G51460. [O64896-2]
DR EnsemblPlants; AT5G51460.3; AT5G51460.3; AT5G51460. [O64896-1]
DR EnsemblPlants; AT5G51460.4; AT5G51460.4; AT5G51460. [O64896-2]
DR EnsemblPlants; AT5G51460.5; AT5G51460.5; AT5G51460. [O64896-1]
DR GeneID; 835220; -.
DR Gramene; AT5G51460.1; AT5G51460.1; AT5G51460. [O64896-1]
DR Gramene; AT5G51460.2; AT5G51460.2; AT5G51460. [O64896-2]
DR Gramene; AT5G51460.3; AT5G51460.3; AT5G51460. [O64896-1]
DR Gramene; AT5G51460.4; AT5G51460.4; AT5G51460. [O64896-2]
DR Gramene; AT5G51460.5; AT5G51460.5; AT5G51460. [O64896-1]
DR KEGG; ath:AT5G51460; -.
DR Araport; AT5G51460; -.
DR TAIR; locus:2153082; AT5G51460.
DR eggNOG; KOG1050; Eukaryota.
DR HOGENOM; CLU_037265_1_0_1; -.
DR InParanoid; O64896; -.
DR OMA; MIKSQPV; -.
DR PhylomeDB; O64896; -.
DR BioCyc; ARA:AT5G51460-MON; -.
DR UniPathway; UPA00299; -.
DR PRO; PR:O64896; -.
DR Proteomes; UP000006548; Chromosome 5.
DR ExpressionAtlas; O64896; baseline and differential.
DR Genevisible; O64896; AT.
DR GO; GO:0004805; F:trehalose-phosphatase activity; IDA:TAIR.
DR GO; GO:0005992; P:trehalose biosynthetic process; IDA:TAIR.
DR Gene3D; 3.40.50.1000; -; 1.
DR InterPro; IPR036412; HAD-like_sf.
DR InterPro; IPR006379; HAD-SF_hydro_IIB.
DR InterPro; IPR023214; HAD_sf.
DR InterPro; IPR044651; OTSB-like.
DR InterPro; IPR003337; Trehalose_PPase.
DR PANTHER; PTHR43768; PTHR43768; 1.
DR Pfam; PF02358; Trehalose_PPase; 1.
DR SUPFAM; SSF56784; SSF56784; 1.
DR TIGRFAMs; TIGR01484; HAD-SF-IIB; 1.
DR TIGRFAMs; TIGR00685; T6PP; 1.
PE 1: Evidence at protein level;
KW Alternative splicing; Hydrolase; Reference proteome; Stress response.
FT CHAIN 1..385
FT /note="Trehalose-phosphate phosphatase A"
FT /id="PRO_0000417643"
FT REGION 1..21
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT VAR_SEQ 99
FT /note="Missing (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:14593172"
FT /id="VSP_043862"
SQ SEQUENCE 385 AA; 43192 MW; DE21BB6F83782CF9 CRC64;
MDMKSGHSSP VMTDSPPISN SRLTIRQNRL PYSSAAATAI SQNNNLLLTV PRKKTGILDD
VKSNGWLDAM KSSSPPPTIL NKDNLSNDAT DMTYREWMQL KYPSALTSFE KIMSFAKGKR
IALFLDYDGT LSPIVEEPDC AYMSSAMRSA VQNVAKYFPT AIISGRSRDK VYEFVNLSEL
YYAGSHGMDI MSPAGESLNH EHSRTVSVYE QGKDVNLFQP ASEFLPMIDK VLCSLIESTK
DIKGVKVEDN KFCISVHYRN VEEKNWTLVA QCVDDVIRTY PKLRLTHGRK VLEIRPVIDW
DKGKAVTFLL ESLGLNNCED VLPIYVGDDR TDEDAFKVLR DGPNHGYGIL VSAVPKDSNA
FYSLRDPSEV MEFLKSLVTW KRSMG
