TPPC2_DROME
ID TPPC2_DROME Reviewed; 139 AA.
AC Q9VUZ1; Q8SYG9;
DT 21-SEP-2011, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-2002, sequence version 2.
DT 03-AUG-2022, entry version 137.
DE RecName: Full=Probable trafficking protein particle complex subunit 2;
DE AltName: Full=TRAPP subunit 20 ortholog {ECO:0000312|FlyBase:FBgn0266724};
GN Name=Trs20 {ECO:0000312|FlyBase:FBgn0266724};
GN Synonyms=l(3)72Dh {ECO:0000312|FlyBase:FBgn0266724};
GN ORFNames=CG5161 {ECO:0000312|FlyBase:FBgn0266724};
OS Drosophila melanogaster (Fruit fly).
OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea;
OC Drosophilidae; Drosophila; Sophophora.
OX NCBI_TaxID=7227;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Berkeley;
RX PubMed=10731132; DOI=10.1126/science.287.5461.2185;
RA Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D.,
RA Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F.,
RA George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N.,
RA Sutton G.G., Wortman J.R., Yandell M.D., Zhang Q., Chen L.X., Brandon R.C.,
RA Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C.,
RA Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A.,
RA An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A.,
RA Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V.,
RA Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J.,
RA Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E.,
RA Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B.,
RA Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I.,
RA Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C.,
RA Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S.,
RA Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M.,
RA Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M.,
RA Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D.,
RA Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F.,
RA Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D.,
RA Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A.,
RA Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C.,
RA McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C.,
RA Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L.,
RA Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R.,
RA Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V.,
RA Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F.,
RA Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J.,
RA Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R.,
RA Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y.,
RA Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T.,
RA Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S.,
RA Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W.,
RA Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M.,
RA Venter J.C.;
RT "The genome sequence of Drosophila melanogaster.";
RL Science 287:2185-2195(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=Berkeley;
RX PubMed=12537572; DOI=10.1186/gb-2002-3-12-research0083;
RA Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S.,
RA Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E.,
RA Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P.,
RA Bettencourt B.R., Celniker S.E., de Grey A.D.N.J., Drysdale R.A.,
RA Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M.,
RA Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E.;
RT "Annotation of the Drosophila melanogaster euchromatic genome: a systematic
RT review.";
RL Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=Berkeley; TISSUE=Embryo;
RX PubMed=12537569; DOI=10.1186/gb-2002-3-12-research0080;
RA Stapleton M., Carlson J.W., Brokstein P., Yu C., Champe M., George R.A.,
RA Guarin H., Kronmiller B., Pacleb J.M., Park S., Wan K.H., Rubin G.M.,
RA Celniker S.E.;
RT "A Drosophila full-length cDNA resource.";
RL Genome Biol. 3:RESEARCH0080.1-RESEARCH0080.8(2002).
RN [4]
RP FUNCTION.
RX PubMed=16862146; DOI=10.1038/ncbl439;
RA Saleh M.-C., van Rij R.P., Hekele A., Gillis A., Foley E., O'Farrell P.H.,
RA Andino R.;
RT "The endocytic pathway mediates cell entry of dsRNA to induce RNAi
RT silencing.";
RL Nat. Cell Biol. 8:793-802(2006).
RN [5]
RP IDENTIFICATION AS PROBABLE TRAPP COMPLEX COMPONENT.
RX PubMed=19934220; DOI=10.1242/jcs.054536;
RA Robinett C.C., Giansanti M.G., Gatti M., Fuller M.T.;
RT "TRAPPII is required for cleavage furrow ingression and localization of
RT Rab11 in dividing male meiotic cells of Drosophila.";
RL J. Cell Sci. 122:4526-4534(2009).
CC -!- FUNCTION: May play a role in vesicular transport from endoplasmic
CC reticulum to Golgi. Involved in dsRNA uptake.
CC {ECO:0000269|PubMed:16862146}.
CC -!- SUBUNIT: Part of the multisubunit TRAPP (transport protein particle)
CC complex. {ECO:0000250|UniProtKB:P0DI81, ECO:0000303|PubMed:19934220}.
CC -!- INTERACTION:
CC Q9VUZ1; Q7K2Q8: Trs31; NbExp=5; IntAct=EBI-157653, EBI-195337;
CC -!- SUBCELLULAR LOCATION: Cytoplasm, perinuclear region
CC {ECO:0000250|UniProtKB:P0DI81}. Endoplasmic reticulum
CC {ECO:0000250|UniProtKB:P0DI81}. Golgi apparatus
CC {ECO:0000250|UniProtKB:P0DI81}.
CC -!- SIMILARITY: Belongs to the TRAPP small subunits family. Sedlin
CC subfamily. {ECO:0000305}.
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DR EMBL; AE014296; AAF49531.2; -; Genomic_DNA.
DR EMBL; AY071561; AAL49183.1; -; mRNA.
DR RefSeq; NP_648841.1; NM_140584.3.
DR PDB; 7B6D; EM; 4.27 A; E=1-139.
DR PDB; 7B6R; EM; 5.80 A; G=1-139.
DR PDB; 7B6X; EM; 3.60 A; E=1-139.
DR PDB; 7B70; EM; 4.00 A; E=1-139.
DR PDBsum; 7B6D; -.
DR PDBsum; 7B6R; -.
DR PDBsum; 7B6X; -.
DR PDBsum; 7B70; -.
DR AlphaFoldDB; Q9VUZ1; -.
DR SMR; Q9VUZ1; -.
DR BioGRID; 65077; 5.
DR IntAct; Q9VUZ1; 3.
DR STRING; 7227.FBpp0075246; -.
DR PaxDb; Q9VUZ1; -.
DR PRIDE; Q9VUZ1; -.
DR DNASU; 39769; -.
DR EnsemblMetazoa; FBtr0075491; FBpp0075246; FBgn0266724.
DR GeneID; 39769; -.
DR KEGG; dme:Dmel_CG5161; -.
DR UCSC; CG5161-RA; d. melanogaster.
DR CTD; 39769; -.
DR FlyBase; FBgn0266724; Trs20.
DR VEuPathDB; VectorBase:FBgn0266724; -.
DR eggNOG; KOG3487; Eukaryota.
DR GeneTree; ENSGT00510000047168; -.
DR HOGENOM; CLU_085828_0_2_1; -.
DR InParanoid; Q9VUZ1; -.
DR OMA; VDKFNQW; -.
DR OrthoDB; 1426075at2759; -.
DR PhylomeDB; Q9VUZ1; -.
DR Reactome; R-DME-204005; COPII-mediated vesicle transport.
DR Reactome; R-DME-8876198; RAB GEFs exchange GTP for GDP on RABs.
DR BioGRID-ORCS; 39769; 1 hit in 3 CRISPR screens.
DR GenomeRNAi; 39769; -.
DR PRO; PR:Q9VUZ1; -.
DR Proteomes; UP000000803; Chromosome 3L.
DR Bgee; FBgn0266724; Expressed in saliva-secreting gland and 27 other tissues.
DR ExpressionAtlas; Q9VUZ1; baseline and differential.
DR Genevisible; Q9VUZ1; DM.
DR GO; GO:0005737; C:cytoplasm; IBA:GO_Central.
DR GO; GO:0005783; C:endoplasmic reticulum; IEA:UniProtKB-SubCell.
DR GO; GO:0005634; C:nucleus; IBA:GO_Central.
DR GO; GO:0048471; C:perinuclear region of cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0030008; C:TRAPP complex; ISS:FlyBase.
DR GO; GO:1990071; C:TRAPPII protein complex; IDA:FlyBase.
DR GO; GO:1990072; C:TRAPPIII protein complex; IDA:FlyBase.
DR GO; GO:0033227; P:dsRNA transport; IMP:FlyBase.
DR GO; GO:0006888; P:endoplasmic reticulum to Golgi vesicle-mediated transport; ISS:FlyBase.
DR GO; GO:0048193; P:Golgi vesicle transport; IC:FlyBase.
DR GO; GO:0007283; P:spermatogenesis; IMP:FlyBase.
DR InterPro; IPR011012; Longin-like_dom_sf.
DR InterPro; IPR006722; Sedlin.
DR PANTHER; PTHR12403; PTHR12403; 1.
DR Pfam; PF04628; Sedlin_N; 1.
DR SUPFAM; SSF64356; SSF64356; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Cytoplasm; Endoplasmic reticulum; ER-Golgi transport;
KW Golgi apparatus; Reference proteome; Transport.
FT CHAIN 1..139
FT /note="Probable trafficking protein particle complex
FT subunit 2"
FT /id="PRO_0000412460"
SQ SEQUENCE 139 AA; 16646 MW; F25AC651C6FDCD05 CRC64;
MSTYYFVIVG QNDNPIYEKE FSTVNKELRK EDHRHLTQFI AHAALDLVDE HKWKTANMQL
KSIDRFNQWF VSAFITASQI RFIIVHDNKN DEGIKNFFNE MYDTYIKNSM NAFYRINTPI
KSPMFEKKSE IFGRKYLLS
