ID   TPPII_ORYSJ             Reviewed;        1359 AA.
AC   Q6ESI7; A0A0P0VMN4; Q6ESI6;
DT   11-JUN-2014, integrated into UniProtKB/Swiss-Prot.
DT   16-AUG-2004, sequence version 1.
DT   03-AUG-2022, entry version 129.
DE   RecName: Full=Tripeptidyl-peptidase 2;
DE            EC=3.4.14.10 {ECO:0000250|UniProtKB:F4JVN6};
DE   AltName: Full=Tripeptidyl-peptidase II;
DE            Short=TPPII;
GN   Name=TPP2; OrderedLocusNames=Os02g0664300, LOC_Os02g44520;
GN   ORFNames=P0461B08.4-1, P0461B08.4-2;
OS   Oryza sativa subsp. japonica (Rice).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; BOP clade;
OC   Oryzoideae; Oryzeae; Oryzinae; Oryza; Oryza sativa.
OX   NCBI_TaxID=39947;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=cv. Nipponbare;
RX   PubMed=16100779; DOI=10.1038/nature03895;
RG   International rice genome sequencing project (IRGSP);
RT   "The map-based sequence of the rice genome.";
RL   Nature 436:793-800(2005).
RN   [2]
RP   GENOME REANNOTATION.
RC   STRAIN=cv. Nipponbare;
RX   PubMed=18089549; DOI=10.1093/nar/gkm978;
RG   The rice annotation project (RAP);
RT   "The rice annotation project database (RAP-DB): 2008 update.";
RL   Nucleic Acids Res. 36:D1028-D1033(2008).
RN   [3]
RP   GENOME REANNOTATION.
RC   STRAIN=cv. Nipponbare;
RX   PubMed=24280374; DOI=10.1186/1939-8433-6-4;
RA   Kawahara Y., de la Bastide M., Hamilton J.P., Kanamori H., McCombie W.R.,
RA   Ouyang S., Schwartz D.C., Tanaka T., Wu J., Zhou S., Childs K.L.,
RA   Davidson R.M., Lin H., Quesada-Ocampo L., Vaillancourt B., Sakai H.,
RA   Lee S.S., Kim J., Numa H., Itoh T., Buell C.R., Matsumoto T.;
RT   "Improvement of the Oryza sativa Nipponbare reference genome using next
RT   generation sequence and optical map data.";
RL   Rice 6:4-4(2013).
RN   [4]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   STRAIN=cv. Nipponbare;
RX   PubMed=12869764; DOI=10.1126/science.1081288;
RG   The rice full-length cDNA consortium;
RT   "Collection, mapping, and annotation of over 28,000 cDNA clones from
RT   japonica rice.";
RL   Science 301:376-379(2003).
CC   -!- FUNCTION: Serine protease that may function in the proteasome pathway.
CC       {ECO:0000250|UniProtKB:F4JVN6}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=Release of an N-terminal tripeptide from a polypeptide.;
CC         EC=3.4.14.10; Evidence={ECO:0000250|UniProtKB:F4JVN6};
CC   -!- SIMILARITY: Belongs to the peptidase S8 family. {ECO:0000305}.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=BAD28384.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR   EMBL; AP005108; BAD28383.1; -; Genomic_DNA.
DR   EMBL; AP005108; BAD28384.1; ALT_SEQ; Genomic_DNA.
DR   EMBL; AP008208; BAF09575.1; -; Genomic_DNA.
DR   EMBL; AP014958; BAS80168.1; -; Genomic_DNA.
DR   EMBL; AK067099; -; NOT_ANNOTATED_CDS; mRNA.
DR   RefSeq; XP_015625917.1; XM_015770431.1.
DR   RefSeq; XP_015625918.1; XM_015770432.1.
DR   AlphaFoldDB; Q6ESI7; -.
DR   SMR; Q6ESI7; -.
DR   BioGRID; 799274; 1.
DR   STRING; 4530.OS02T0664300-01; -.
DR   MEROPS; S08.A56; -.
DR   PaxDb; Q6ESI7; -.
DR   PRIDE; Q6ESI7; -.
DR   EnsemblPlants; Os02t0664300-01; Os02t0664300-01; Os02g0664300.
DR   GeneID; 4330237; -.
DR   Gramene; Os02t0664300-01; Os02t0664300-01; Os02g0664300.
DR   KEGG; osa:4330237; -.
DR   eggNOG; KOG1114; Eukaryota.
DR   InParanoid; Q6ESI7; -.
DR   OMA; SLRDFQC; -.
DR   OrthoDB; 156304at2759; -.
DR   Proteomes; UP000000763; Chromosome 2.
DR   Proteomes; UP000059680; Chromosome 2.
DR   ExpressionAtlas; Q6ESI7; baseline and differential.
DR   Genevisible; Q6ESI7; OS.
DR   GO; GO:0005829; C:cytosol; IBA:GO_Central.
DR   GO; GO:0004177; F:aminopeptidase activity; IEA:UniProtKB-KW.
DR   GO; GO:0003729; F:mRNA binding; IEA:EnsemblPlants.
DR   GO; GO:0004252; F:serine-type endopeptidase activity; IEA:InterPro.
DR   GO; GO:0008240; F:tripeptidyl-peptidase activity; IBA:GO_Central.
DR   GO; GO:0006508; P:proteolysis; IEA:UniProtKB-KW.
DR   CDD; cd04857; Peptidases_S8_Tripeptidyl_Aminopeptidase_II; 1.
DR   Gene3D; 3.40.50.200; -; 1.
DR   InterPro; IPR000209; Peptidase_S8/S53_dom.
DR   InterPro; IPR036852; Peptidase_S8/S53_dom_sf.
DR   InterPro; IPR023828; Peptidase_S8_Ser-AS.
DR   InterPro; IPR015500; Peptidase_S8_subtilisin-rel.
DR   InterPro; IPR022229; Peptidase_S8A_TPPII.
DR   InterPro; IPR034051; TPP_II_domain.
DR   Pfam; PF00082; Peptidase_S8; 1.
DR   Pfam; PF12580; TPPII; 1.
DR   PRINTS; PR00723; SUBTILISIN.
DR   SUPFAM; SSF52743; SSF52743; 1.
DR   PROSITE; PS51892; SUBTILASE; 1.
DR   PROSITE; PS00138; SUBTILASE_SER; 1.
PE   2: Evidence at transcript level;
KW   Aminopeptidase; Hydrolase; Protease; Reference proteome; Serine protease.
FT   CHAIN           1..1359
FT                   /note="Tripeptidyl-peptidase 2"
FT                   /id="PRO_0000429314"
FT   DOMAIN          102..600
FT                   /note="Peptidase S8"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01240"
FT   REGION          45..81
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        62..77
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   ACT_SITE        126
FT                   /note="Charge relay system"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01240"
FT   ACT_SITE        353
FT                   /note="Charge relay system"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01240"
FT   ACT_SITE        539
FT                   /note="Charge relay system"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01240"
FT   CONFLICT        245
FT                   /note="S -> C (in Ref. 4; AK067099)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        1199
FT                   /note="D -> E (in Ref. 4; AK067099)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        1249
FT                   /note="A -> S (in Ref. 4; AK067099)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   1359 AA;  148633 MW;  29EFC31A30302C2E CRC64;
     MWHLRGRSSV TAAAAAALHK PVAHLRLLLA VSAWSVPAAA SNVAAASTTT RGGPSPSAGV
     APRAMPSSSS SPPSAAEGTT AAAGGFRLTE PSFLESLMPK KEIGVDRFLA AHPEYDGRGA
     LIAIFDSGVD PAAAGLQTTS DGKPKILDVI DCTGSGDVDT SKVVKADDDG SIVGASGTHL
     TINPSWKNPS QEWHVGCKLV YELFTDTLTS RLKKERKKKW DEHNQEAISE ALKQLNEFEK
     KHSKSDDAKQ KMAREDLQSR LEYLRKQAEG YDDRGPVIDI VAWHDGDVWR VAVDTQGLEG
     NKNCGKLADF VPLTNYRLER KFGIFSKLDA CSFVANIYDD GNLVSIVTDC SPHATHVAGI
     AAAFHPDEPL LNGVAPGAQL ISCKIGDTRL GSMETGTGLV RALIAAVEHK CDLINMSYGE
     PTLLPDYGRF IDLASEVVDK HRIIFISSAG NNGPALNTVG APGGTSSSII GVGAYVSPAM
     AAGAHCVVQA PAEGMEYTWS SRGPTADGDL GVSISAPGGA VAPVPTWTLQ SRMLMNGTSM
     SSPSACGGVA LLVSAMKAEG IPLSPYTVRK AIENTAASIS DVPEEKLTTG HGLLQVDRAF
     EYAQQAKELP LVSYRISINQ VGKPTSKLRG IYLRGSNTCR QTSEWTVQLD PKFHEDASNM
     EQLVPFEECL QLHSTDSSVI KIPEYIMVTN NGRTFNIVVN PVNISSGLHY YEVYGIDCKA
     PWRGPIFRVP ITVIKPIALS GEPPALTLSN LSFKSGHIER RFINVPIGAS WVEVTMRTSA
     FDTPRRFFLD TVQICPLKRP IKWEAVVTFS SPSLKNFSFP VEGGLTLELS IAQFWSSGIA
     SHEPTCVDFE IVFHGISVDQ KIIGLDGSEA PVRVVARSLL ASERLVPVAT LNKVKTPYRP
     VESNLCSLPP SRDRLPSGKQ IIALTLTYKF KLEDGAEIKP RVPLLNNRIY DNKFESQYYR
     ISDSNKCVYS SGDVYPNYVK LSKGEYTLQL YIRHDNVQLL EKLKQLVLFI ERKLEKKDFI
     QLSFYSEPDG PTVGNGTFKS SILVPGEPEA FYVGPPSREK LPKNVLPGSV LVGSITYGAV
     SSFSKKDDQN QHAPASYSIS YLIPPSKVDN DKEKGVSSGR KSISERLDDE VRDTKIKFLS
     GFNQETEDDK SSWTALVASL KPEYPKYTPL LAKILECIVQ KATSDDKFSH QKEIIAAADE
     VVDSIDKEDL AKSLSLKPDP EDEEAQKNKK KMEETRDQLA DALYQKGLAL AEIESLKTDE
     STEASAKDVF EENYKELIKW VDAKTTKYGS LTVLRERRCG RLGTALKVLN DMIQDDSEQP
     KKRLYDLKIQ LIEEIGWVHV SAYEKQWMHV RFPPSLPPF