TPPP2_MOUSE
ID TPPP2_MOUSE Reviewed; 170 AA.
AC Q0P5Y3;
DT 29-MAY-2007, integrated into UniProtKB/Swiss-Prot.
DT 19-SEP-2006, sequence version 1.
DT 03-AUG-2022, entry version 97.
DE RecName: Full=Tubulin polymerization-promoting protein family member 2 {ECO:0000303|PubMed:30680919};
GN Name=Tppp2 {ECO:0000303|PubMed:30680919, ECO:0000312|MGI:MGI:2684923};
GN Synonyms=Gm77 {ECO:0000312|MGI:MGI:2684923};
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC TISSUE=Testis;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [2]
RP FUNCTION, SUBCELLULAR LOCATION, DISRUPTION PHENOTYPE, AND TISSUE
RP SPECIFICITY.
RX PubMed=30680919; DOI=10.1111/jcmm.14149;
RA Zhu F., Yan P., Zhang J., Cui Y., Zheng M., Cheng Y., Guo Y., Yang X.,
RA Guo X., Zhu H.;
RT "Deficiency of TPPP2, a factor linked to oligoasthenozoospermia, causes
RT subfertility in male mice.";
RL J. Cell. Mol. Med. 23:2583-2594(2019).
CC -!- FUNCTION: Probable regulator of microtubule dynamics required for sperm
CC motility (Probable). In contrast to other members of the family, has no
CC microtubule bundling activity (By similarity).
CC {ECO:0000250|UniProtKB:P59282, ECO:0000305|PubMed:30680919}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm, cytosol
CC {ECO:0000250|UniProtKB:P59282}. Cell projection, cilium, flagellum
CC {ECO:0000269|PubMed:30680919}. Note=Present in the middle piece of
CC sperm tail. {ECO:0000269|PubMed:30680919}.
CC -!- TISSUE SPECIFICITY: Only expressed in male reproductive organs,
CC including testis (PubMed:30680919). Expressed in elongating spermatids
CC at stages IV-VIII of the seminiferous epithelial cycle in testis and in
CC mature sperm in the epididymis (PubMed:30680919).
CC {ECO:0000269|PubMed:30680919}.
CC -!- DISRUPTION PHENOTYPE: Male subfertility with a significantly decreased
CC sperm count and motility (PubMed:30680919). Sperm shows increased
CC irregular mitochondria lacking lamellar cristae, abnormal expression of
CC electron transfer chain molecules, lower ATP levels, decreased
CC mitochondrial membrane potential and increased apoptotic index
CC (PubMed:30680919). {ECO:0000269|PubMed:30680919}.
CC -!- SIMILARITY: Belongs to the TPPP family. {ECO:0000305}.
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DR EMBL; BC049709; AAH49709.1; -; mRNA.
DR CCDS; CCDS49487.1; -.
DR RefSeq; NP_001122106.1; NM_001128634.1.
DR RefSeq; XP_006518907.1; XM_006518844.3.
DR AlphaFoldDB; Q0P5Y3; -.
DR SMR; Q0P5Y3; -.
DR STRING; 10090.ENSMUSP00000008957; -.
DR iPTMnet; Q0P5Y3; -.
DR PhosphoSitePlus; Q0P5Y3; -.
DR MaxQB; Q0P5Y3; -.
DR PaxDb; Q0P5Y3; -.
DR PRIDE; Q0P5Y3; -.
DR ProteomicsDB; 297511; -.
DR Antibodypedia; 70; 60 antibodies from 16 providers.
DR Ensembl; ENSMUST00000008957; ENSMUSP00000008957; ENSMUSG00000008813.
DR Ensembl; ENSMUST00000177625; ENSMUSP00000136454; ENSMUSG00000008813.
DR GeneID; 219038; -.
DR KEGG; mmu:219038; -.
DR UCSC; uc007tnm.1; mouse.
DR CTD; 122664; -.
DR MGI; MGI:2684923; Tppp2.
DR VEuPathDB; HostDB:ENSMUSG00000008813; -.
DR eggNOG; KOG4070; Eukaryota.
DR GeneTree; ENSGT00940000153875; -.
DR HOGENOM; CLU_091734_0_0_1; -.
DR InParanoid; Q0P5Y3; -.
DR OMA; RAKNART; -.
DR OrthoDB; 1317210at2759; -.
DR PhylomeDB; Q0P5Y3; -.
DR TreeFam; TF314440; -.
DR BioGRID-ORCS; 219038; 2 hits in 70 CRISPR screens.
DR PRO; PR:Q0P5Y3; -.
DR Proteomes; UP000000589; Chromosome 14.
DR RNAct; Q0P5Y3; protein.
DR Bgee; ENSMUSG00000008813; Expressed in spermatid and 13 other tissues.
DR GO; GO:0005829; C:cytosol; ISO:MGI.
DR GO; GO:0036126; C:sperm flagellum; IDA:UniProtKB.
DR GO; GO:0015631; F:tubulin binding; ISO:MGI.
DR GO; GO:0030154; P:cell differentiation; IEA:UniProtKB-KW.
DR GO; GO:0032273; P:positive regulation of protein polymerization; IBA:GO_Central.
DR GO; GO:1901317; P:regulation of flagellated sperm motility; IMP:UniProtKB.
DR GO; GO:0007283; P:spermatogenesis; IEA:UniProtKB-KW.
DR InterPro; IPR011992; EF-hand-dom_pair.
DR InterPro; IPR008907; P25-alpha.
DR InterPro; IPR030794; TPPP2.
DR PANTHER; PTHR12932; PTHR12932; 1.
DR PANTHER; PTHR12932:SF21; PTHR12932:SF21; 1.
DR Pfam; PF05517; p25-alpha; 1.
DR SUPFAM; SSF47473; SSF47473; 1.
PE 2: Evidence at transcript level;
KW Cell projection; Cilium; Cytoplasm; Differentiation; Flagellum;
KW Reference proteome; Spermatogenesis.
FT CHAIN 1..170
FT /note="Tubulin polymerization-promoting protein family
FT member 2"
FT /id="PRO_0000289004"
FT REGION 105..170
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 105..123
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 124..148
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 170 AA; 18681 MW; 35A3C79403F3F067 CRC64;
MASEAERTFH RFAVFGESSS SSKEITNKNF SKLCKDCDIM DGKAVTSTDV DIVFSKVKAK
NARTINFQQF QEAMKELGQK RFKGKNPDEA LQGVFKLMEG KDPATTGVTK STTVGGVDRL
TDTSKYTGTH KERFDESGKG KGIEGREETT DNSGYVSGYK GAGTYDKKNQ
