TPPP3_MOUSE
ID TPPP3_MOUSE Reviewed; 176 AA.
AC Q9CRB6; Q3TUZ0;
DT 12-FEB-2003, integrated into UniProtKB/Swiss-Prot.
DT 01-JUN-2001, sequence version 1.
DT 03-AUG-2022, entry version 139.
DE RecName: Full=Tubulin polymerization-promoting protein family member 3 {ECO:0000303|PubMed:19235716};
GN Name=Tppp3 {ECO:0000303|PubMed:19235716, ECO:0000312|MGI:MGI:1915221};
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=C57BL/6J, and NOD; TISSUE=Embryo, and Tongue;
RX PubMed=16141072; DOI=10.1126/science.1112014;
RA Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N.,
RA Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K.,
RA Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.,
RA Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R.,
RA Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T.,
RA Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A.,
RA Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B.,
RA Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M.,
RA Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S.,
RA Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E.,
RA Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D.,
RA Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M.,
RA Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H.,
RA Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V.,
RA Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S.,
RA Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H.,
RA Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N.,
RA Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F.,
RA Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G.,
RA Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z.,
RA Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C.,
RA Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y.,
RA Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S.,
RA Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K.,
RA Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R.,
RA van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H.,
RA Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M.,
RA Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C.,
RA Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S.,
RA Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K.,
RA Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M.,
RA Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C.,
RA Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A.,
RA Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.;
RT "The transcriptional landscape of the mammalian genome.";
RL Science 309:1559-1563(2005).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC TISSUE=Colon;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [3]
RP DEVELOPMENTAL STAGE.
RX PubMed=19235716; DOI=10.1002/dvdy.21865;
RA Staverosky J.A., Pryce B.A., Watson S.S., Schweitzer R.;
RT "Tubulin polymerization-promoting protein family member 3, Tppp3, is a
RT specific marker of the differentiating tendon sheath and synovial joints.";
RL Dev. Dyn. 238:685-692(2009).
RN [4]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Brain, Brown adipose tissue, Heart, Kidney, Lung, Pancreas, Spleen,
RC and Testis;
RX PubMed=21183079; DOI=10.1016/j.cell.2010.12.001;
RA Huttlin E.L., Jedrychowski M.P., Elias J.E., Goswami T., Rad R.,
RA Beausoleil S.A., Villen J., Haas W., Sowa M.E., Gygi S.P.;
RT "A tissue-specific atlas of mouse protein phosphorylation and expression.";
RL Cell 143:1174-1189(2010).
RN [5]
RP FUNCTION, TISSUE SPECIFICITY, AND INDUCTION.
RX PubMed=29901777; DOI=10.1093/biolre/ioy136;
RA Shukla V., Popli P., Kaushal J.B., Gupta K., Dwivedi A.;
RT "Uterine TPPP3 plays important role in embryo implantation via modulation
RT of beta-catenin.";
RL Biol. Reprod. 99:982-999(2018).
RN [6]
RP FUNCTION.
RX PubMed=30667362; DOI=10.1530/joe-18-0459;
RA Shukla V., Kaushal J.B., Sankhwar P., Manohar M., Dwivedi A.;
RT "Inhibition of TPPP3 attenuates beta-catenin/NF-kappaB/COX-2 signaling in
RT endometrial stromal cells and impairs decidualization.";
RL J. Endocrinol. 240:417-429(2019).
RN [7]
RP STRUCTURE BY NMR OF 1-138.
RG RIKEN structural genomics initiative (RSGI);
RT "Solution structure of mouse CGI-38 protein.";
RL Submitted (JUL-2005) to the PDB data bank.
CC -!- FUNCTION: Regulator of microtubule dynamic that has microtubule
CC bundling activity (By similarity). Required for embryo implantation;
CC possibly by regulating beta-catenin (PubMed:29901777). Also required
CC for decidualization via regulation of beta-catenin (PubMed:30667362).
CC {ECO:0000250|UniProtKB:Q9BW30, ECO:0000269|PubMed:29901777,
CC ECO:0000269|PubMed:30667362}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000250|UniProtKB:Q9BW30}.
CC Cytoplasm, cytoskeleton {ECO:0000250|UniProtKB:Q9BW30}.
CC -!- DEVELOPMENTAL STAGE: Expressed in the connective tissues of
CC differentiating tendons and synovial joints (PubMed:19235716).
CC Expressed in the uterus during window of implantation and early
CC pregnancy (at protein level) (PubMed:29901777).
CC {ECO:0000269|PubMed:19235716, ECO:0000269|PubMed:29901777}.
CC -!- INDUCTION: Up-regulated by estradiol. {ECO:0000269|PubMed:29901777}.
CC -!- SIMILARITY: Belongs to the TPPP family. {ECO:0000305}.
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DR EMBL; AK009771; BAB26493.1; -; mRNA.
DR EMBL; AK012437; BAB28237.1; -; mRNA.
DR EMBL; AK155018; BAE32991.1; -; mRNA.
DR EMBL; AK160507; BAE35831.1; -; mRNA.
DR EMBL; BC010788; AAH10788.1; -; mRNA.
DR CCDS; CCDS22602.1; -.
DR RefSeq; NP_080757.1; NM_026481.3.
DR RefSeq; XP_006531387.1; XM_006531324.3.
DR PDB; 1WLM; NMR; -; A=1-138.
DR PDBsum; 1WLM; -.
DR AlphaFoldDB; Q9CRB6; -.
DR BMRB; Q9CRB6; -.
DR SMR; Q9CRB6; -.
DR STRING; 10090.ENSMUSP00000014990; -.
DR iPTMnet; Q9CRB6; -.
DR PhosphoSitePlus; Q9CRB6; -.
DR EPD; Q9CRB6; -.
DR MaxQB; Q9CRB6; -.
DR PaxDb; Q9CRB6; -.
DR PeptideAtlas; Q9CRB6; -.
DR PRIDE; Q9CRB6; -.
DR ProteomicsDB; 259069; -.
DR Antibodypedia; 29534; 198 antibodies from 25 providers.
DR Ensembl; ENSMUST00000014990; ENSMUSP00000014990; ENSMUSG00000014846.
DR Ensembl; ENSMUST00000176419; ENSMUSP00000134807; ENSMUSG00000014846.
DR Ensembl; ENSMUST00000177126; ENSMUSP00000135040; ENSMUSG00000014846.
DR GeneID; 67971; -.
DR KEGG; mmu:67971; -.
DR UCSC; uc009ndc.2; mouse.
DR CTD; 51673; -.
DR MGI; MGI:1915221; Tppp3.
DR VEuPathDB; HostDB:ENSMUSG00000014846; -.
DR eggNOG; KOG4070; Eukaryota.
DR GeneTree; ENSGT00940000153875; -.
DR HOGENOM; CLU_091734_0_0_1; -.
DR InParanoid; Q9CRB6; -.
DR OMA; PKASGHE; -.
DR OrthoDB; 1317210at2759; -.
DR PhylomeDB; Q9CRB6; -.
DR TreeFam; TF314440; -.
DR BioGRID-ORCS; 67971; 1 hit in 72 CRISPR screens.
DR EvolutionaryTrace; Q9CRB6; -.
DR PRO; PR:Q9CRB6; -.
DR Proteomes; UP000000589; Chromosome 8.
DR RNAct; Q9CRB6; protein.
DR Bgee; ENSMUSG00000014846; Expressed in facial nucleus and 248 other tissues.
DR Genevisible; Q9CRB6; MM.
DR GO; GO:0005874; C:microtubule; IEA:UniProtKB-KW.
DR GO; GO:0097427; C:microtubule bundle; ISO:MGI.
DR GO; GO:0048471; C:perinuclear region of cytoplasm; ISO:MGI.
DR GO; GO:0015631; F:tubulin binding; ISS:UniProtKB.
DR GO; GO:0046697; P:decidualization; ISS:UniProtKB.
DR GO; GO:0007566; P:embryo implantation; IMP:UniProtKB.
DR GO; GO:0001578; P:microtubule bundle formation; ISS:UniProtKB.
DR GO; GO:0046785; P:microtubule polymerization; IEA:InterPro.
DR GO; GO:0032273; P:positive regulation of protein polymerization; IBA:GO_Central.
DR InterPro; IPR011992; EF-hand-dom_pair.
DR InterPro; IPR008907; P25-alpha.
DR InterPro; IPR030795; TPPP3.
DR PANTHER; PTHR12932; PTHR12932; 1.
DR PANTHER; PTHR12932:SF16; PTHR12932:SF16; 1.
DR Pfam; PF05517; p25-alpha; 1.
DR SUPFAM; SSF47473; SSF47473; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Acetylation; Cytoplasm; Cytoskeleton; Microtubule;
KW Reference proteome.
FT INIT_MET 1
FT /note="Removed"
FT /evidence="ECO:0000250|UniProtKB:Q9BW30"
FT CHAIN 2..176
FT /note="Tubulin polymerization-promoting protein family
FT member 3"
FT /id="PRO_0000221139"
FT REGION 132..152
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 132..151
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 2
FT /note="N-acetylalanine"
FT /evidence="ECO:0000250|UniProtKB:Q9BW30"
FT HELIX 10..18
FT /evidence="ECO:0007829|PDB:1WLM"
FT STRAND 27..31
FT /evidence="ECO:0007829|PDB:1WLM"
FT HELIX 32..41
FT /evidence="ECO:0007829|PDB:1WLM"
FT STRAND 47..50
FT /evidence="ECO:0007829|PDB:1WLM"
FT HELIX 52..62
FT /evidence="ECO:0007829|PDB:1WLM"
FT STRAND 68..71
FT /evidence="ECO:0007829|PDB:1WLM"
FT HELIX 72..81
FT /evidence="ECO:0007829|PDB:1WLM"
FT HELIX 83..86
FT /evidence="ECO:0007829|PDB:1WLM"
FT STRAND 87..89
FT /evidence="ECO:0007829|PDB:1WLM"
FT HELIX 92..103
FT /evidence="ECO:0007829|PDB:1WLM"
SQ SEQUENCE 176 AA; 18965 MW; 05CD1F57E263B156 CRC64;
MAASTDIAGL EESFRKFAIH GDPKASGQEM NGKNWAKLCK DCKVADGKAV TGTDVDIVFS
KVKAKSARVI NYEEFKKALE ELATKRFKGK SKEEAFDAIC QLIAGKEPAN IGVTKAKTGG
AVDRLTDTSK YTGSHKERFD ESGKGKGIAG RQDILDDSGY VSAYKNAGTY DAKVKK
