ID   TPP_ENCCU               Reviewed;         718 AA.
AC   Q8SSL0; O96721;
DT   31-AUG-2004, integrated into UniProtKB/Swiss-Prot.
DT   01-JUN-2002, sequence version 1.
DT   25-MAY-2022, entry version 96.
DE   RecName: Full=Probable trehalose-phosphatase;
DE            EC=3.1.3.12;
DE   AltName: Full=Trehalose-6-phosphate phosphatase;
DE            Short=TPP;
GN   OrderedLocusNames=ECU01_0870;
OS   Encephalitozoon cuniculi (strain GB-M1) (Microsporidian parasite).
OC   Eukaryota; Fungi; Fungi incertae sedis; Microsporidia; Unikaryonidae;
OC   Encephalitozoon.
OX   NCBI_TaxID=284813;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RA   Duffieux F., Peyret P., Roe B.A., Vivares C.P.;
RT   "Putative trehalose 6-phosphate phosphatase from the chromosome I of
RT   Encephalitozoon cuniculi (Microspora).";
RL   Submitted (JUN-1998) to the EMBL/GenBank/DDBJ databases.
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=GB-M1;
RX   PubMed=11157783; DOI=10.1101/gr.164301;
RA   Peyret P., Katinka M.D., Duprat S., Duffieux F., Barbe V., Barbazanges M.,
RA   Weissenbach J., Saurin W., Vivares C.P.;
RT   "Sequence and analysis of chromosome I of the amitochondriate intracellular
RT   parasite Encephalitozoon cuniculi (Microspora).";
RL   Genome Res. 11:198-207(2001).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=GB-M1;
RX   PubMed=11719806; DOI=10.1038/35106579;
RA   Katinka M.D., Duprat S., Cornillot E., Metenier G., Thomarat F.,
RA   Prensier G., Barbe V., Peyretaillade E., Brottier P., Wincker P.,
RA   Delbac F., El Alaoui H., Peyret P., Saurin W., Gouy M., Weissenbach J.,
RA   Vivares C.P.;
RT   "Genome sequence and gene compaction of the eukaryote parasite
RT   Encephalitozoon cuniculi.";
RL   Nature 414:450-453(2001).
RN   [4]
RP   IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS], AND
RP   DEVELOPMENTAL STAGE.
RX   PubMed=16691553; DOI=10.1002/pmic.200500796;
RA   Brosson D., Kuhn L., Delbac F., Garin J., Vivares C.P., Texier C.;
RT   "Proteomic analysis of the eukaryotic parasite Encephalitozoon cuniculi
RT   (microsporidia): a reference map for proteins expressed in late sporogonial
RT   stages.";
RL   Proteomics 6:3625-3635(2006).
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=alpha,alpha-trehalose 6-phosphate + H2O = alpha,alpha-
CC         trehalose + phosphate; Xref=Rhea:RHEA:23420, ChEBI:CHEBI:15377,
CC         ChEBI:CHEBI:16551, ChEBI:CHEBI:43474, ChEBI:CHEBI:58429; EC=3.1.3.12;
CC   -!- DEVELOPMENTAL STAGE: Expressed in late sporogonial stages.
CC       {ECO:0000269|PubMed:16691553}.
CC   -!- SIMILARITY: In the N-terminal section; belongs to the
CC       glycosyltransferase 20 family. {ECO:0000305}.
CC   -!- SIMILARITY: In the C-terminal section; belongs to the trehalose
CC       phosphatase family. {ECO:0000305}.
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DR   EMBL; AJ006825; CAA07262.1; -; Genomic_DNA.
DR   EMBL; AL391737; CAD24957.1; -; Genomic_DNA.
DR   RefSeq; XP_965922.1; XM_960829.1.
DR   AlphaFoldDB; Q8SSL0; -.
DR   SMR; Q8SSL0; -.
DR   STRING; 284813.Q8SSL0; -.
DR   CAZy; GT20; Glycosyltransferase Family 20.
DR   GeneID; 860263; -.
DR   KEGG; ecu:ECU01_0870; -.
DR   VEuPathDB; MicrosporidiaDB:ECU01_0870; -.
DR   HOGENOM; CLU_002351_3_3_1; -.
DR   InParanoid; Q8SSL0; -.
DR   OMA; RMAYYSI; -.
DR   OrthoDB; 772297at2759; -.
DR   Proteomes; UP000000819; Chromosome I.
DR   GO; GO:0004805; F:trehalose-phosphatase activity; IEA:UniProtKB-EC.
DR   GO; GO:0005992; P:trehalose biosynthetic process; IEA:InterPro.
DR   Gene3D; 3.40.50.1000; -; 1.
DR   InterPro; IPR001830; Glyco_trans_20.
DR   InterPro; IPR036412; HAD-like_sf.
DR   InterPro; IPR023214; HAD_sf.
DR   InterPro; IPR003337; Trehalose_PPase.
DR   PANTHER; PTHR10788; PTHR10788; 2.
DR   Pfam; PF00982; Glyco_transf_20; 1.
DR   Pfam; PF02358; Trehalose_PPase; 1.
DR   SUPFAM; SSF56784; SSF56784; 1.
DR   TIGRFAMs; TIGR00685; T6PP; 1.
PE   1: Evidence at protein level;
KW   Hydrolase; Reference proteome.
FT   CHAIN           1..718
FT                   /note="Probable trehalose-phosphatase"
FT                   /id="PRO_0000122507"
FT   REGION          449..470
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   CONFLICT        421
FT                   /note="R -> K (in Ref. 1; CAA07262)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   718 AA;  82064 MW;  B82FC7D318854CD5 CRC64;
     MKIIVAAEEL PICASRMEPS EAIKNWISTP LKKIPHPDKA SKIQIEFGDR SKESLHLFAK
     PHFMVPDMLD DDEMFFVGAP GFYSPEEFSD EDCRRIEESM KAYRCYPIFQ KRVKYSKMIE
     EILGKNTYEF VQSNFDHANM FARYKEYNTR WHEKIMEIYE EGDVVWVMDH SLFLLPGMLG
     NSIPVGMSAS VPFSSLLKCI PFWEQIFSSI LCCRYIEFNE SSSKESFDLL VSQKTGFCMG
     DPGYKDIREP LTCVGKKGID KDVLLKMSSE VHEFEGLGKG KVILLPSDSQ THLLGVEAYL
     SRYGKEITVL FLRTRVLNGD SDKQAEVMRL REYLEINYKV LSREFTPASD PEFISMLKRC
     DLCHCPEVAD VCSLFGIPVV RNNPYDFFDI ADEINENLMQ RGEGSKEGSS EVIGKMEWKK
     RFMTSLLSIS GMEYDVDLEP KEPRIRSSLS MDQTGHKKVD AKKKPGIRKK NREKEEAVEI
     ILNNKEDANA ARIVNDFKKS KARTLVMDYD GTLTNIVARP PMAAPTQEIK DLLIRLGKIC
     RVVISTGRSV EDCDKFFPKE IEVFAEHGAC HRIDGKWKEG GTFPQKDLAW RIGQFFLART
     PGSELERKKT GYAFHFRNVS PLIGVKQARA LFELLMRVCK DYVKKGNHVI EVRSSKKSCA
     MEKIEEGFVL CAGDDVADED MFDVCKGYTI KVGDQSTSAA YRVKDPENFR MLLGRLLE